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- PMID: 28375175
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Article: Mechanisms of Iron Uptake from Ferric Phosphate Nanoparticles in Human Intestinal Caco-2 Cells
Title | Mechanisms of Iron Uptake from Ferric Phosphate Nanoparticles in Human Intestinal Caco-2 Cells |
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Authors | |
Keywords | Bioavailability Caco-2 cells DMT1 Endocytosis Nano iron |
Issue Date | 2017 |
Publisher | MDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/nutrients/ |
Citation | Nutrients, 2017, v. 9 n. 4, article no. 359 How to Cite? |
Abstract | Food fortification programs to reduce iron deficiency anemia require bioavailable forms of iron that do not cause adverse organoleptic effects. Rodent studies show that nano-sized ferric phosphate (NP-FePO4) is as bioavailable as ferrous sulfate, but there is controversy over the mechanism of absorption. We undertook in vitro studies to examine this using a Caco-2 cell model and simulated gastrointestinal (GI) digestion. Supernatant iron concentrations increased inversely with pH, and iron uptake into Caco-2 cells was 2–3 fold higher when NP-FePO4 was digested at pH 1 compared to pH 2. The size and distribution of NP-FePO4 particles during GI digestion was examined using transmission electron microscopy. The d50 of the particle distribution was 413 nm. Using disc centrifugal sedimentation, a high degree of agglomeration in NP-FePO4 following simulated GI digestion was observed, with only 20% of the particles ≤1000 nm. In Caco-2 cells, divalent metal transporter-1 (DMT1) and endocytosis inhibitors demonstrated that NP-FePO4 was mainly absorbed via DMT1. Small particles may be absorbed by clathrin-mediated endocytosis and micropinocytosis. These findings should be considered when assessing the potential of iron nanoparticles for food fortification. © 2017 by the authors. |
Persistent Identifier | http://hdl.handle.net/10722/266546 |
ISSN | 2023 Impact Factor: 4.8 2023 SCImago Journal Rankings: 1.301 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Perfecto, AP | - |
dc.contributor.author | Elgy, CE | - |
dc.contributor.author | Valsami-Jones, EVJ | - |
dc.contributor.author | Sharp, PS | - |
dc.contributor.author | Hilty, FH | - |
dc.contributor.author | Fairweather-Tait, SJ | - |
dc.date.accessioned | 2019-01-21T02:03:29Z | - |
dc.date.available | 2019-01-21T02:03:29Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Nutrients, 2017, v. 9 n. 4, article no. 359 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | http://hdl.handle.net/10722/266546 | - |
dc.description.abstract | Food fortification programs to reduce iron deficiency anemia require bioavailable forms of iron that do not cause adverse organoleptic effects. Rodent studies show that nano-sized ferric phosphate (NP-FePO4) is as bioavailable as ferrous sulfate, but there is controversy over the mechanism of absorption. We undertook in vitro studies to examine this using a Caco-2 cell model and simulated gastrointestinal (GI) digestion. Supernatant iron concentrations increased inversely with pH, and iron uptake into Caco-2 cells was 2–3 fold higher when NP-FePO4 was digested at pH 1 compared to pH 2. The size and distribution of NP-FePO4 particles during GI digestion was examined using transmission electron microscopy. The d50 of the particle distribution was 413 nm. Using disc centrifugal sedimentation, a high degree of agglomeration in NP-FePO4 following simulated GI digestion was observed, with only 20% of the particles ≤1000 nm. In Caco-2 cells, divalent metal transporter-1 (DMT1) and endocytosis inhibitors demonstrated that NP-FePO4 was mainly absorbed via DMT1. Small particles may be absorbed by clathrin-mediated endocytosis and micropinocytosis. These findings should be considered when assessing the potential of iron nanoparticles for food fortification. © 2017 by the authors. | - |
dc.language | eng | - |
dc.publisher | MDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/nutrients/ | - |
dc.relation.ispartof | Nutrients | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Bioavailability | - |
dc.subject | Caco-2 cells | - |
dc.subject | DMT1 | - |
dc.subject | Endocytosis | - |
dc.subject | Nano iron | - |
dc.title | Mechanisms of Iron Uptake from Ferric Phosphate Nanoparticles in Human Intestinal Caco-2 Cells | - |
dc.type | Article | - |
dc.identifier.email | Perfecto, AP: perfecto@hku.hk | - |
dc.identifier.authority | Perfecto, AP=rp02514 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3390/nu9040359 | - |
dc.identifier.pmid | 28375175 | - |
dc.identifier.scopus | eid_2-s2.0-85017141669 | - |
dc.identifier.hkuros | 296683 | - |
dc.identifier.volume | 9 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | article no. 359 | - |
dc.identifier.epage | article no. 359 | - |
dc.identifier.isi | WOS:000401355600044 | - |
dc.publisher.place | Switzerland | - |
dc.identifier.issnl | 2072-6643 | - |