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Article: The influence of the blood handling process on the measurement of circulating TGF-β1

TitleThe influence of the blood handling process on the measurement of circulating TGF-β1
Authors
KeywordsTransforming growth factor
Platelet
Plasma
Serum
Issue Date2012
Citation
European Cytokine Network, 2012, v. 23, n. 1, p. 1-6 How to Cite?
AbstractIn order to evaluate the impact of blood sample handling processes on circulating TGF-β1 levels, blood specimens were obtained from 13 healthy volunteers using different handling processes (kept at room temperature (RT) or on ice before centrifugation, using different centrifugal forces). TGF-β1 levels were measured using an enzyme-linked immunosorbent assay. A paired-T test was used for statistical analysis. The TGF-β1 level in on-ice serum was significantly lower than that in room-temperature serum (P<0.001), and both were significantly higher than that found in on-ice plasma (P<0.001). Compared with on-ice plasma samples, the longer the samples were kept at RT, the higher the levels of TGF-β1 in plasma (P=0.268, 0.040, and 0.0015 for 5 min, 30 min, and 60 min in RT, respectively). Compared with plasma centrifuged at 2,500×g for 30 min, the TGF-β1 levels were much lower than those found in plasma centrifuged at 1,200×g for 10 min (P=0.003); and a double centrifugation before TGF-β1 detection, significantly decreased the level (P<0.001). It is suggested that the optimal sampling conditions for the detection of TGF-β1 should be plasma prepared on ice and spun down at a higher centrifugal force.
Persistent Identifierhttp://hdl.handle.net/10722/266932
ISSN
2018 Impact Factor: 1.875
2023 SCImago Journal Rankings: 0.667
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhao, Lujun-
dc.contributor.authorWang, Luhua-
dc.contributor.authorJi, Wei-
dc.contributor.authorLei, Mingfang-
dc.contributor.authorYang, Weizhi-
dc.contributor.authorKong, Feng Ming-
dc.date.accessioned2019-01-31T07:20:01Z-
dc.date.available2019-01-31T07:20:01Z-
dc.date.issued2012-
dc.identifier.citationEuropean Cytokine Network, 2012, v. 23, n. 1, p. 1-6-
dc.identifier.issn1148-5493-
dc.identifier.urihttp://hdl.handle.net/10722/266932-
dc.description.abstractIn order to evaluate the impact of blood sample handling processes on circulating TGF-β1 levels, blood specimens were obtained from 13 healthy volunteers using different handling processes (kept at room temperature (RT) or on ice before centrifugation, using different centrifugal forces). TGF-β1 levels were measured using an enzyme-linked immunosorbent assay. A paired-T test was used for statistical analysis. The TGF-β1 level in on-ice serum was significantly lower than that in room-temperature serum (P<0.001), and both were significantly higher than that found in on-ice plasma (P<0.001). Compared with on-ice plasma samples, the longer the samples were kept at RT, the higher the levels of TGF-β1 in plasma (P=0.268, 0.040, and 0.0015 for 5 min, 30 min, and 60 min in RT, respectively). Compared with plasma centrifuged at 2,500×g for 30 min, the TGF-β1 levels were much lower than those found in plasma centrifuged at 1,200×g for 10 min (P=0.003); and a double centrifugation before TGF-β1 detection, significantly decreased the level (P<0.001). It is suggested that the optimal sampling conditions for the detection of TGF-β1 should be plasma prepared on ice and spun down at a higher centrifugal force.-
dc.languageeng-
dc.relation.ispartofEuropean Cytokine Network-
dc.subjectTransforming growth factor-
dc.subjectPlatelet-
dc.subjectPlasma-
dc.subjectSerum-
dc.titleThe influence of the blood handling process on the measurement of circulating TGF-β1-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1684/ecn.2012.0298-
dc.identifier.pmid22456089-
dc.identifier.scopuseid_2-s2.0-84859307803-
dc.identifier.volume23-
dc.identifier.issue1-
dc.identifier.spage1-
dc.identifier.epage6-
dc.identifier.isiWOS:000302753800001-
dc.identifier.issnl1148-5493-

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