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- Publisher Website: 10.1684/ecn.2012.0298
- Scopus: eid_2-s2.0-84859307803
- PMID: 22456089
- WOS: WOS:000302753800001
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Article: The influence of the blood handling process on the measurement of circulating TGF-β1
Title | The influence of the blood handling process on the measurement of circulating TGF-β1 |
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Authors | |
Keywords | Transforming growth factor Platelet Plasma Serum |
Issue Date | 2012 |
Citation | European Cytokine Network, 2012, v. 23, n. 1, p. 1-6 How to Cite? |
Abstract | In order to evaluate the impact of blood sample handling processes on circulating TGF-β1 levels, blood specimens were obtained from 13 healthy volunteers using different handling processes (kept at room temperature (RT) or on ice before centrifugation, using different centrifugal forces). TGF-β1 levels were measured using an enzyme-linked immunosorbent assay. A paired-T test was used for statistical analysis. The TGF-β1 level in on-ice serum was significantly lower than that in room-temperature serum (P<0.001), and both were significantly higher than that found in on-ice plasma (P<0.001). Compared with on-ice plasma samples, the longer the samples were kept at RT, the higher the levels of TGF-β1 in plasma (P=0.268, 0.040, and 0.0015 for 5 min, 30 min, and 60 min in RT, respectively). Compared with plasma centrifuged at 2,500×g for 30 min, the TGF-β1 levels were much lower than those found in plasma centrifuged at 1,200×g for 10 min (P=0.003); and a double centrifugation before TGF-β1 detection, significantly decreased the level (P<0.001). It is suggested that the optimal sampling conditions for the detection of TGF-β1 should be plasma prepared on ice and spun down at a higher centrifugal force. |
Persistent Identifier | http://hdl.handle.net/10722/266932 |
ISSN | 2018 Impact Factor: 1.875 2023 SCImago Journal Rankings: 0.667 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhao, Lujun | - |
dc.contributor.author | Wang, Luhua | - |
dc.contributor.author | Ji, Wei | - |
dc.contributor.author | Lei, Mingfang | - |
dc.contributor.author | Yang, Weizhi | - |
dc.contributor.author | Kong, Feng Ming | - |
dc.date.accessioned | 2019-01-31T07:20:01Z | - |
dc.date.available | 2019-01-31T07:20:01Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | European Cytokine Network, 2012, v. 23, n. 1, p. 1-6 | - |
dc.identifier.issn | 1148-5493 | - |
dc.identifier.uri | http://hdl.handle.net/10722/266932 | - |
dc.description.abstract | In order to evaluate the impact of blood sample handling processes on circulating TGF-β1 levels, blood specimens were obtained from 13 healthy volunteers using different handling processes (kept at room temperature (RT) or on ice before centrifugation, using different centrifugal forces). TGF-β1 levels were measured using an enzyme-linked immunosorbent assay. A paired-T test was used for statistical analysis. The TGF-β1 level in on-ice serum was significantly lower than that in room-temperature serum (P<0.001), and both were significantly higher than that found in on-ice plasma (P<0.001). Compared with on-ice plasma samples, the longer the samples were kept at RT, the higher the levels of TGF-β1 in plasma (P=0.268, 0.040, and 0.0015 for 5 min, 30 min, and 60 min in RT, respectively). Compared with plasma centrifuged at 2,500×g for 30 min, the TGF-β1 levels were much lower than those found in plasma centrifuged at 1,200×g for 10 min (P=0.003); and a double centrifugation before TGF-β1 detection, significantly decreased the level (P<0.001). It is suggested that the optimal sampling conditions for the detection of TGF-β1 should be plasma prepared on ice and spun down at a higher centrifugal force. | - |
dc.language | eng | - |
dc.relation.ispartof | European Cytokine Network | - |
dc.subject | Transforming growth factor | - |
dc.subject | Platelet | - |
dc.subject | Plasma | - |
dc.subject | Serum | - |
dc.title | The influence of the blood handling process on the measurement of circulating TGF-β1 | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1684/ecn.2012.0298 | - |
dc.identifier.pmid | 22456089 | - |
dc.identifier.scopus | eid_2-s2.0-84859307803 | - |
dc.identifier.volume | 23 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 6 | - |
dc.identifier.isi | WOS:000302753800001 | - |
dc.identifier.issnl | 1148-5493 | - |