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Conference Paper: MERS: from Middle East to the East and beyond
Title | MERS: from Middle East to the East and beyond |
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Authors | |
Issue Date | 2017 |
Publisher | Kenes International Organizers of Congresses S.A. |
Citation | 10th World Congress of the World Society for Pediatric Infectious Diseases (WSPID 2017), Shenzhen, China, 2-5 December 2017 How to Cite? |
Abstract | Since its first appearance in 2012, MERS has affected >25 countries in four continents with >2,000 cases and a high fatality rate of >30%. A novel lineage C betacoronavirus, MERS coronavirus (MERS-CoV), has been confirmed to be the etiological agent. Human dipeptidyl peptidase 4 was found to be the cellular receptor for MERS-CoV. Subsequent detection of MERS-CoV and its antibodies in dromedaries in various countries in the Middle East and North Africa have implied that these animals are probably the reservoir for MERS-CoV. Other lineage C betacoronaviruses in bats (e.g. Tylonycteris bat CoV HKU4, Pipistrellus bat CoV HKU5) and hedgehogs were found to be closely related to MERS-CoV. So far, detection of MERS-CoV and discoveries of its closely related CoVs are most efficiently achieved through RT-PCR. Although RT-PCR is highly sensitive, its turn-around-time is about four hours and the test requires expensive equipment, stringent laboratory set-up and personal attention to prevent laboratory PCR product cross contamination which may lead to false-positive results. Recently, we have developed a monoclonal antibody-based rapid nucleocapsid protein detection assay for on-site diagnosis of MERS-CoV, which can be finished in 30 minutes. In addition to human and camel samples, we examined the usefulness of this rapid assay to detect other lineage C betacoronaviruses closely related to MERS-CoV in bats. The rapid MERS-CoV nucleocapsid protein detection assay was tested positive in 24 (88.9%) of 27 Tylonycteris bat CoV HKU4 RNA-positive alimentary samples of Tylonycteris pachypus and 4 (19.0%) of 21 Pipistrellus bat CoV HKU5 RNA-positive alimentary samples of Pipistrellus abramus. The rapid assay was tested negative in all 51 alimentary samples RNA-positive for alphacoronaviruses (Rhinolophus bat CoV HKU2, Myotis bat CoV HKU6, Miniopterus bat CoV HKU8 and Hipposideros batCoV HKU10) and 32 alimentary samples positive for lineage B (SARS-related Rhinolophus bat CoV HKU3) and lineage D (Rousettus bat CoV HKU9) betacoronaviruses. This rapid assay will facilitate rapid on-site diagnosis of MERS and rapid on-site screening of animal samples for ancestors of MERS-CoV and tracking transmission in the related bat species from the Middle East to the East and beyond. |
Description | WSPID Symposium 10 Emerging challenges in infectious diseases (MERS, Yellow Fever; Influenza) - no. WSPD7-1299 |
Persistent Identifier | http://hdl.handle.net/10722/268131 |
DC Field | Value | Language |
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dc.contributor.author | ]Woo, PCY | - |
dc.date.accessioned | 2019-03-18T02:28:42Z | - |
dc.date.available | 2019-03-18T02:28:42Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | 10th World Congress of the World Society for Pediatric Infectious Diseases (WSPID 2017), Shenzhen, China, 2-5 December 2017 | - |
dc.identifier.uri | http://hdl.handle.net/10722/268131 | - |
dc.description | WSPID Symposium 10 Emerging challenges in infectious diseases (MERS, Yellow Fever; Influenza) - no. WSPD7-1299 | - |
dc.description.abstract | Since its first appearance in 2012, MERS has affected >25 countries in four continents with >2,000 cases and a high fatality rate of >30%. A novel lineage C betacoronavirus, MERS coronavirus (MERS-CoV), has been confirmed to be the etiological agent. Human dipeptidyl peptidase 4 was found to be the cellular receptor for MERS-CoV. Subsequent detection of MERS-CoV and its antibodies in dromedaries in various countries in the Middle East and North Africa have implied that these animals are probably the reservoir for MERS-CoV. Other lineage C betacoronaviruses in bats (e.g. Tylonycteris bat CoV HKU4, Pipistrellus bat CoV HKU5) and hedgehogs were found to be closely related to MERS-CoV. So far, detection of MERS-CoV and discoveries of its closely related CoVs are most efficiently achieved through RT-PCR. Although RT-PCR is highly sensitive, its turn-around-time is about four hours and the test requires expensive equipment, stringent laboratory set-up and personal attention to prevent laboratory PCR product cross contamination which may lead to false-positive results. Recently, we have developed a monoclonal antibody-based rapid nucleocapsid protein detection assay for on-site diagnosis of MERS-CoV, which can be finished in 30 minutes. In addition to human and camel samples, we examined the usefulness of this rapid assay to detect other lineage C betacoronaviruses closely related to MERS-CoV in bats. The rapid MERS-CoV nucleocapsid protein detection assay was tested positive in 24 (88.9%) of 27 Tylonycteris bat CoV HKU4 RNA-positive alimentary samples of Tylonycteris pachypus and 4 (19.0%) of 21 Pipistrellus bat CoV HKU5 RNA-positive alimentary samples of Pipistrellus abramus. The rapid assay was tested negative in all 51 alimentary samples RNA-positive for alphacoronaviruses (Rhinolophus bat CoV HKU2, Myotis bat CoV HKU6, Miniopterus bat CoV HKU8 and Hipposideros batCoV HKU10) and 32 alimentary samples positive for lineage B (SARS-related Rhinolophus bat CoV HKU3) and lineage D (Rousettus bat CoV HKU9) betacoronaviruses. This rapid assay will facilitate rapid on-site diagnosis of MERS and rapid on-site screening of animal samples for ancestors of MERS-CoV and tracking transmission in the related bat species from the Middle East to the East and beyond. | - |
dc.language | eng | - |
dc.publisher | Kenes International Organizers of Congresses S.A. | - |
dc.relation.ispartof | 10th World Congress of The World Society for Pediatric Infectious Diseases | - |
dc.title | MERS: from Middle East to the East and beyond | - |
dc.type | Conference_Paper | - |
dc.identifier.email | ]Woo, PCY: pcywoo@hkucc.hku.hk | - |
dc.identifier.authority | ]Woo, PCY=rp00430 | - |
dc.identifier.hkuros | 293399 | - |