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- Publisher Website: 10.1083/jcb.201109001
- Scopus: eid_2-s2.0-84859991512
- PMID: 22371557
- WOS: WOS:000301323100004
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Article: Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction
Title | Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction |
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Authors | |
Issue Date | 2012 |
Citation | Journal of Cell Biology, 2012, v. 196, n. 5, p. 563-571 How to Cite? |
Abstract | The KMN network (named according to the acronym for KNL1, Mis12, and Ndc80) and the more recently identified Ska complex (Ska1-3) have been shown to mediate kinetochore (KT)-microtubule (MT) attachments. How these two complexes cooperate to achieve stable end-on attachments remains unknown. In this paper, we show that Aurora B negatively regulates the localization of the Ska complex to KTs and that recruitment of the Ska complex to KTs depends on the KMN network. We identified interactions between members of the KMN and Ska complexes and demonstrated that these interactions are regulated by Aurora B. Aurora B directly phosphorylated Ska1 and Ska3 in vitro, and expression of phosphomimetic mutants of Ska1 and Ska3 impaired Ska KT recruitment and formation of stable KT-MT fibers (K-fibers), disrupting mitotic progression. We propose that Aurora B phosphorylation antagonizes the interaction between the Ska complex and the KMN network, thereby controlling Ska recruitment to KTs and stabilization of KT-MT attachments. © 2012 Chan et al. |
Persistent Identifier | http://hdl.handle.net/10722/268530 |
ISSN | 2023 Impact Factor: 7.4 2023 SCImago Journal Rankings: 3.717 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chan, Ying Wai | - |
dc.contributor.author | Jeyaprakash, A. Arockia | - |
dc.contributor.author | Nigg, Erich A. | - |
dc.contributor.author | Santamaria, Anna | - |
dc.date.accessioned | 2019-03-25T07:59:58Z | - |
dc.date.available | 2019-03-25T07:59:58Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Journal of Cell Biology, 2012, v. 196, n. 5, p. 563-571 | - |
dc.identifier.issn | 0021-9525 | - |
dc.identifier.uri | http://hdl.handle.net/10722/268530 | - |
dc.description.abstract | The KMN network (named according to the acronym for KNL1, Mis12, and Ndc80) and the more recently identified Ska complex (Ska1-3) have been shown to mediate kinetochore (KT)-microtubule (MT) attachments. How these two complexes cooperate to achieve stable end-on attachments remains unknown. In this paper, we show that Aurora B negatively regulates the localization of the Ska complex to KTs and that recruitment of the Ska complex to KTs depends on the KMN network. We identified interactions between members of the KMN and Ska complexes and demonstrated that these interactions are regulated by Aurora B. Aurora B directly phosphorylated Ska1 and Ska3 in vitro, and expression of phosphomimetic mutants of Ska1 and Ska3 impaired Ska KT recruitment and formation of stable KT-MT fibers (K-fibers), disrupting mitotic progression. We propose that Aurora B phosphorylation antagonizes the interaction between the Ska complex and the KMN network, thereby controlling Ska recruitment to KTs and stabilization of KT-MT attachments. © 2012 Chan et al. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Cell Biology | - |
dc.title | Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction | - |
dc.type | Article | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1083/jcb.201109001 | - |
dc.identifier.pmid | 22371557 | - |
dc.identifier.pmcid | PMC3307695 | - |
dc.identifier.scopus | eid_2-s2.0-84859991512 | - |
dc.identifier.volume | 196 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 563 | - |
dc.identifier.epage | 571 | - |
dc.identifier.eissn | 1540-8140 | - |
dc.identifier.isi | WOS:000301323100004 | - |
dc.identifier.issnl | 0021-9525 | - |