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- Publisher Website: 10.1039/c7ob01009e
- Scopus: eid_2-s2.0-85021630258
- PMID: 28569333
- WOS: WOS:000404012300003
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Article: Photo-controlled cell-specific metabolic labeling of RNA
Title | Photo-controlled cell-specific metabolic labeling of RNA |
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Authors | |
Issue Date | 2017 |
Citation | Organic and Biomolecular Chemistry, 2017, v. 15, n. 24, p. 5117-5120 How to Cite? |
Abstract | © The Royal Society of Chemistry 2017. Elucidating gene expression programs within a cell-specific manner is a grand challenge for biologists. Harder still is the ability to have kinetic control over such experiments. Metabolic labeling with bioorthogonally-functionalized metabolic intermediates provides a means to profile RNA expression in a cell-specific manner, but there is still a lack of kinetic resolution. Herein we present the synthesis and evaluation of photocaged metabolic uracil intermediates. We compare the photo-decaging properties and demonstrate their utility in metabolic labeling experiments in a cell-specific manner. We anticipate that our approach will have far-reaching impact as it provides control over tagging of nascent RNA. |
Persistent Identifier | http://hdl.handle.net/10722/270373 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 0.607 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Feng, C. | - |
dc.contributor.author | Li, Y. | - |
dc.contributor.author | Spitale, R. C. | - |
dc.date.accessioned | 2019-05-27T03:57:27Z | - |
dc.date.available | 2019-05-27T03:57:27Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Organic and Biomolecular Chemistry, 2017, v. 15, n. 24, p. 5117-5120 | - |
dc.identifier.issn | 1477-0520 | - |
dc.identifier.uri | http://hdl.handle.net/10722/270373 | - |
dc.description.abstract | © The Royal Society of Chemistry 2017. Elucidating gene expression programs within a cell-specific manner is a grand challenge for biologists. Harder still is the ability to have kinetic control over such experiments. Metabolic labeling with bioorthogonally-functionalized metabolic intermediates provides a means to profile RNA expression in a cell-specific manner, but there is still a lack of kinetic resolution. Herein we present the synthesis and evaluation of photocaged metabolic uracil intermediates. We compare the photo-decaging properties and demonstrate their utility in metabolic labeling experiments in a cell-specific manner. We anticipate that our approach will have far-reaching impact as it provides control over tagging of nascent RNA. | - |
dc.language | eng | - |
dc.relation.ispartof | Organic and Biomolecular Chemistry | - |
dc.title | Photo-controlled cell-specific metabolic labeling of RNA | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1039/c7ob01009e | - |
dc.identifier.pmid | 28569333 | - |
dc.identifier.pmcid | PMC6193551 | - |
dc.identifier.scopus | eid_2-s2.0-85021630258 | - |
dc.identifier.volume | 15 | - |
dc.identifier.issue | 24 | - |
dc.identifier.spage | 5117 | - |
dc.identifier.epage | 5120 | - |
dc.identifier.isi | WOS:000404012300003 | - |
dc.identifier.issnl | 1477-0520 | - |