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Article: Intra‐oral single site comparisons of periodontal and peri‐implant microbiota in health and disease

TitleIntra‐oral single site comparisons of periodontal and peri‐implant microbiota in health and disease
Authors
Keywordsdental implant
etiology
Illumina
oral disease
oral microbiome
Issue Date2019
PublisherWiley for European Association for Osseointegration. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR
Citation
Clinical Oral Implants Research, 2019, v. 30 n. 8, p. 760-776 How to Cite?
AbstractObjective: Periodontitis and peri-implantitis are oral infectious-inflammatory diseases that share similarities in their pathology and etiology. Our objective was to characterize the single-site subgingival and submucosal microbiomes of implant-rehabilitated, partially dentate Chinese subjects (n=18) presenting with both periodontitis and peri-implantitis. Materials and Methods: Subgingival/submucosal plaque samples were collected from four clinically-distinct sites in each subject: peri-implantitis submucosa (DI), periodontal pocket (DT), clinically-healthy (unaffected) peri-implant submucosa (HI), and clinically-healthy (unaffected) subgingival sulcus (HT). The bacterial microbiota present was analysed using Illumina MiSeq sequencing. Results: 26 phyla and 5,726 Operational Taxonomic Units (OTUs, 97% sequence similarity cut-off) were identified. Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, Actinobacteria, Synergistetes, TM7 and Spirochaetes comprised 99.6% of the total reads detected. Bacterial communities within the DI, DT, HI and HT sites shared high levels of taxonomic similarity. 31 ‘core species’ were present in >90% sites; with Streptococcus infantis/mitis/oralis (HMT-070/HMT-071/HMT-638/HMT-677) and Fusobacterium sp. HMT-203/HMT-698 being particularly prevalent and abundant. Beta-diversity analyses (Permanova-test, weighted-UniFrac) revealed the largest variance in the microbiota was at the subject level (46%), followed by periodontal health status (4%). Differing sets of OTUs were associated with periodontitis and peri-implantitis sites, respectively. This included putative ‘periodontopathogens, such as Prevotella, Porphyromonas, Tannerella, Bacteroidetes [G-5] and Treponema spp. Interaction network analysis identified several putative patterns underlying dysbiosis in periodontitis/peri-implantitis sites. Conclusions: Species (OTU) composition of the periodontal and peri-implant microbiota varied widely between subjects. The inter-subject variations in subgingival/submucosal microbiome composition outweighed differences observed between implant versus tooth sites, or between diseased versus healthy (unaffected) peri-implant/periodontal sites.
Persistent Identifierhttp://hdl.handle.net/10722/271206
ISSN
2023 Impact Factor: 4.8
2023 SCImago Journal Rankings: 1.865
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYu, XL-
dc.contributor.authorChan, Y-
dc.contributor.authorZhuang, LF-
dc.contributor.authorLai, HC-
dc.contributor.authorLang, NP-
dc.contributor.authorLeung, WK-
dc.contributor.authorWatt, RM-
dc.date.accessioned2019-06-24T01:05:25Z-
dc.date.available2019-06-24T01:05:25Z-
dc.date.issued2019-
dc.identifier.citationClinical Oral Implants Research, 2019, v. 30 n. 8, p. 760-776-
dc.identifier.issn0905-7161-
dc.identifier.urihttp://hdl.handle.net/10722/271206-
dc.description.abstractObjective: Periodontitis and peri-implantitis are oral infectious-inflammatory diseases that share similarities in their pathology and etiology. Our objective was to characterize the single-site subgingival and submucosal microbiomes of implant-rehabilitated, partially dentate Chinese subjects (n=18) presenting with both periodontitis and peri-implantitis. Materials and Methods: Subgingival/submucosal plaque samples were collected from four clinically-distinct sites in each subject: peri-implantitis submucosa (DI), periodontal pocket (DT), clinically-healthy (unaffected) peri-implant submucosa (HI), and clinically-healthy (unaffected) subgingival sulcus (HT). The bacterial microbiota present was analysed using Illumina MiSeq sequencing. Results: 26 phyla and 5,726 Operational Taxonomic Units (OTUs, 97% sequence similarity cut-off) were identified. Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, Actinobacteria, Synergistetes, TM7 and Spirochaetes comprised 99.6% of the total reads detected. Bacterial communities within the DI, DT, HI and HT sites shared high levels of taxonomic similarity. 31 ‘core species’ were present in >90% sites; with Streptococcus infantis/mitis/oralis (HMT-070/HMT-071/HMT-638/HMT-677) and Fusobacterium sp. HMT-203/HMT-698 being particularly prevalent and abundant. Beta-diversity analyses (Permanova-test, weighted-UniFrac) revealed the largest variance in the microbiota was at the subject level (46%), followed by periodontal health status (4%). Differing sets of OTUs were associated with periodontitis and peri-implantitis sites, respectively. This included putative ‘periodontopathogens, such as Prevotella, Porphyromonas, Tannerella, Bacteroidetes [G-5] and Treponema spp. Interaction network analysis identified several putative patterns underlying dysbiosis in periodontitis/peri-implantitis sites. Conclusions: Species (OTU) composition of the periodontal and peri-implant microbiota varied widely between subjects. The inter-subject variations in subgingival/submucosal microbiome composition outweighed differences observed between implant versus tooth sites, or between diseased versus healthy (unaffected) peri-implant/periodontal sites.-
dc.languageeng-
dc.publisherWiley for European Association for Osseointegration. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR-
dc.relation.ispartofClinical Oral Implants Research-
dc.rightsThis is the peer reviewed version of the following article: [Clinical Oral Implants Research, 2019, v. 30 n. 8, p. 760-776], which has been published in final form at [http://dx.doi.org/10.1111/clr.13459]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectdental implant-
dc.subjectetiology-
dc.subjectIllumina-
dc.subjectoral disease-
dc.subjectoral microbiome-
dc.titleIntra‐oral single site comparisons of periodontal and peri‐implant microbiota in health and disease-
dc.typeArticle-
dc.identifier.emailChan, Y: yukicyk@hku.hk-
dc.identifier.emailLang, NP: nplang@hkucc.hku.hk-
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hk-
dc.identifier.emailWatt, RM: rmwatt@hku.hk-
dc.identifier.authorityChan, Y=rp02228-
dc.identifier.authorityLang, NP=rp00031-
dc.identifier.authorityLeung, WK=rp00019-
dc.identifier.authorityWatt, RM=rp00043-
dc.description.naturepostprint-
dc.identifier.doi10.1111/clr.13459-
dc.identifier.pmid31102416-
dc.identifier.scopuseid_2-s2.0-85067014609-
dc.identifier.hkuros297885-
dc.identifier.volume30-
dc.identifier.issue8-
dc.identifier.spage760-
dc.identifier.epage776-
dc.identifier.isiWOS:000479265600006-
dc.publisher.placeUnited States-
dc.identifier.issnl0905-7161-

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