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- Publisher Website: 10.1111/bjh.15075
- Scopus: eid_2-s2.0-85038429697
- PMID: 29265356
- WOS: WOS:000428370200003
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Article: Molecular detection of minimal residual disease in multiple myeloma
Title | Molecular detection of minimal residual disease in multiple myeloma |
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Authors | |
Keywords | Allele-specific oligonucleotide PCR Digital PCR Minimal residual disease Multiple myeloma Next-generation sequencing |
Issue Date | 2018 |
Publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJH |
Citation | British Journal of Haematology, 2018, v. 181 n. 1, p. 11-26 How to Cite? |
Abstract | Despite the significantly higher complete remission rates and improved survival achieved in the last decade, multiple myeloma (MM) patients continue to relapse due to persistence of minimal residual disease (MRD). Generally, MRD refers to persistence of low levels of disease in the order of one tumour cell in ≥105 normal cells. Currently, molecular and immunophenotypic techniques are employed for MRD detection. This review focuses on MRD detection by molecular techniques, including allele‐specific oligonucleotide polymerase chain reaction (ASO‐PCR), next‐generation sequencing (NGS) and digital PCR (dPCR), in addition to a brief description of, and comparison with, multiparameter flow cytometry. The basic principles, technical advantages and limitations, and the clinical impact of all three molecular techniques are reviewed and compared. They all have a sensitivity of at least 10−5, among which ASO real‐time quantitative PCR is the most well‐standardized, and NGS carries the highest sensitivity and applicability, while dPCR is still under investigation. Furthermore, molecular MRD negativity is a favourable prognostic factor for survival of patients with MM. However, several challenges inherent to molecular detection of MRD still remain to be overcome, particularly false negativity and failure to detect extramedullary disease. Finally, detection of MRD from peripheral blood remains challenging. |
Persistent Identifier | http://hdl.handle.net/10722/271995 |
ISSN | 2023 Impact Factor: 5.1 2023 SCImago Journal Rankings: 1.574 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Bai, Y | - |
dc.contributor.author | Orfao, A | - |
dc.contributor.author | Chim, CS | - |
dc.date.accessioned | 2019-07-20T10:33:38Z | - |
dc.date.available | 2019-07-20T10:33:38Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | British Journal of Haematology, 2018, v. 181 n. 1, p. 11-26 | - |
dc.identifier.issn | 0007-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10722/271995 | - |
dc.description.abstract | Despite the significantly higher complete remission rates and improved survival achieved in the last decade, multiple myeloma (MM) patients continue to relapse due to persistence of minimal residual disease (MRD). Generally, MRD refers to persistence of low levels of disease in the order of one tumour cell in ≥105 normal cells. Currently, molecular and immunophenotypic techniques are employed for MRD detection. This review focuses on MRD detection by molecular techniques, including allele‐specific oligonucleotide polymerase chain reaction (ASO‐PCR), next‐generation sequencing (NGS) and digital PCR (dPCR), in addition to a brief description of, and comparison with, multiparameter flow cytometry. The basic principles, technical advantages and limitations, and the clinical impact of all three molecular techniques are reviewed and compared. They all have a sensitivity of at least 10−5, among which ASO real‐time quantitative PCR is the most well‐standardized, and NGS carries the highest sensitivity and applicability, while dPCR is still under investigation. Furthermore, molecular MRD negativity is a favourable prognostic factor for survival of patients with MM. However, several challenges inherent to molecular detection of MRD still remain to be overcome, particularly false negativity and failure to detect extramedullary disease. Finally, detection of MRD from peripheral blood remains challenging. | - |
dc.language | eng | - |
dc.publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJH | - |
dc.relation.ispartof | British Journal of Haematology | - |
dc.subject | Allele-specific oligonucleotide PCR | - |
dc.subject | Digital PCR | - |
dc.subject | Minimal residual disease | - |
dc.subject | Multiple myeloma | - |
dc.subject | Next-generation sequencing | - |
dc.title | Molecular detection of minimal residual disease in multiple myeloma | - |
dc.type | Article | - |
dc.identifier.email | Chim, CS: jcschim@hku.hk | - |
dc.identifier.authority | Chim, CS=rp00408 | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1111/bjh.15075 | - |
dc.identifier.pmid | 29265356 | - |
dc.identifier.scopus | eid_2-s2.0-85038429697 | - |
dc.identifier.hkuros | 299557 | - |
dc.identifier.volume | 181 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 11 | - |
dc.identifier.epage | 26 | - |
dc.identifier.isi | WOS:000428370200003 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 0007-1048 | - |