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Article: Establishing Pure Cancer Organoid Cultures: Identification, Selection and Verification of Cancer Phenotypes and Genotypes

TitleEstablishing Pure Cancer Organoid Cultures: Identification, Selection and Verification of Cancer Phenotypes and Genotypes
Authors
KeywordsCancer organoids
Stomach
Selection strategies
Disease models
Issue Date2019
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jmb
Citation
Journal of Molecular Biology, 2019, v. 431 n. 15, p. 2884-2893 How to Cite?
AbstractPrecision medicine requires in vitro models which will both faithfully recapitulate the features of an individual's disease and enable drug testing on a wide variety of samples covering the greatest range of phenotypes possible for a particular disease. Organoid technology has immense potential to fulfill this demand, but it will be necessary to develop robust protocols that enable the generation of organoids in a dependable manner from nearly every patient. Here we provide a user's guide, including detailed step-by-step protocols, to the establishment, isolation and verification of gastric cancer organoids. Selection strategies include omission of growth factors, addition of drugs, isolation of distinct phenotypes and generation of monoclonal lines. For confirmation of cancer identity, we use sequencing, drug selection, karyotyping and histology. While we specify these protocols for human gastric cancer organoids here, the methods described are applicable to organoids derived from other tissues as well.
Persistent Identifierhttp://hdl.handle.net/10722/272009
ISSN
2023 Impact Factor: 4.7
2023 SCImago Journal Rankings: 2.212
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWallaschek, N-
dc.contributor.authorNiklas, C-
dc.contributor.authorPompaiah, M-
dc.contributor.authorWiegering, A-
dc.contributor.authorGermer, C-
dc.contributor.authorKircher, S-
dc.contributor.authorBrändlein, S-
dc.contributor.authorMaurus, K-
dc.contributor.authorRosenwald, A-
dc.contributor.authorYan, HHN-
dc.contributor.authorLeung, SY-
dc.contributor.authorBartfeld, S-
dc.date.accessioned2019-07-20T10:33:54Z-
dc.date.available2019-07-20T10:33:54Z-
dc.date.issued2019-
dc.identifier.citationJournal of Molecular Biology, 2019, v. 431 n. 15, p. 2884-2893-
dc.identifier.issn0022-2836-
dc.identifier.urihttp://hdl.handle.net/10722/272009-
dc.description.abstractPrecision medicine requires in vitro models which will both faithfully recapitulate the features of an individual's disease and enable drug testing on a wide variety of samples covering the greatest range of phenotypes possible for a particular disease. Organoid technology has immense potential to fulfill this demand, but it will be necessary to develop robust protocols that enable the generation of organoids in a dependable manner from nearly every patient. Here we provide a user's guide, including detailed step-by-step protocols, to the establishment, isolation and verification of gastric cancer organoids. Selection strategies include omission of growth factors, addition of drugs, isolation of distinct phenotypes and generation of monoclonal lines. For confirmation of cancer identity, we use sequencing, drug selection, karyotyping and histology. While we specify these protocols for human gastric cancer organoids here, the methods described are applicable to organoids derived from other tissues as well.-
dc.languageeng-
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jmb-
dc.relation.ispartofJournal of Molecular Biology-
dc.subjectCancer organoids-
dc.subjectStomach-
dc.subjectSelection strategies-
dc.subjectDisease models-
dc.titleEstablishing Pure Cancer Organoid Cultures: Identification, Selection and Verification of Cancer Phenotypes and Genotypes-
dc.typeArticle-
dc.identifier.emailYan, HHN: yanhelen@hkucc.hku.hk-
dc.identifier.emailLeung, SY: suetyi@hku.hk-
dc.identifier.authorityYan, HHN=rp01994-
dc.identifier.authorityLeung, SY=rp00359-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jmb.2019.05.031-
dc.identifier.pmid31150736-
dc.identifier.scopuseid_2-s2.0-85066794014-
dc.identifier.hkuros298833-
dc.identifier.volume431-
dc.identifier.issue15-
dc.identifier.spage2884-
dc.identifier.epage2893-
dc.identifier.isiWOS:000475997200018-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl0022-2836-

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