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- Publisher Website: 10.1002/cbic.201500239
- Scopus: eid_2-s2.0-84938741118
- PMID: 26052818
- WOS: WOS:000359314400003
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Article: Critical Intermediates Reveal New Biosynthetic Events in the Enigmatic Colibactin Pathway
Title | Critical Intermediates Reveal New Biosynthetic Events in the Enigmatic Colibactin Pathway |
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Authors | |
Keywords | Escherichia coli heterologous expression precolibactin prodrug mechanism colibactin |
Issue Date | 2015 |
Citation | ChemBioChem, 2015, v. 16, n. 12, p. 1715-1719 How to Cite? |
Abstract | © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Colibactin is a potent genotoxin that induces DNA double-strand breaks; it is produced by Escherichia coli strains harboring a pks+ island. However, the structure of this compound remains elusive. Here, using transformation-associated recombination (TAR) cloning to perform heterologous expression, we took advantage of the significantly increased yield of colibactin pathway-related compounds to determine and isolate a series of vital (pre)colibactin intermediates. The chemical structures of compounds 8, 10 and 11 were identified by NMR and MSn analyses. The new 1H-pyrrolo[3,4-c]pyridine-3,6(2H,5H)-dione- and thiazole-containing compound 10 provides new insights regarding the biosynthetic pathway to (pre)colibactin and establishes foundations for future investigation of the intriguing (pre)colibactin structures and its modes of action. |
Persistent Identifier | http://hdl.handle.net/10722/273540 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.809 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Li, Zhong Rui | - |
dc.contributor.author | Li, Yongxin | - |
dc.contributor.author | Lai, Jennifer Y.H. | - |
dc.contributor.author | Tang, Jianqiang | - |
dc.contributor.author | Wang, Bin | - |
dc.contributor.author | Lu, Liang | - |
dc.contributor.author | Zhu, Guoqiang | - |
dc.contributor.author | Wu, Xiyang | - |
dc.contributor.author | Xu, Ying | - |
dc.contributor.author | Qian, Pei Yuan | - |
dc.date.accessioned | 2019-08-12T09:55:52Z | - |
dc.date.available | 2019-08-12T09:55:52Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | ChemBioChem, 2015, v. 16, n. 12, p. 1715-1719 | - |
dc.identifier.issn | 1439-4227 | - |
dc.identifier.uri | http://hdl.handle.net/10722/273540 | - |
dc.description.abstract | © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Colibactin is a potent genotoxin that induces DNA double-strand breaks; it is produced by Escherichia coli strains harboring a pks+ island. However, the structure of this compound remains elusive. Here, using transformation-associated recombination (TAR) cloning to perform heterologous expression, we took advantage of the significantly increased yield of colibactin pathway-related compounds to determine and isolate a series of vital (pre)colibactin intermediates. The chemical structures of compounds 8, 10 and 11 were identified by NMR and MS<sup>n</sup> analyses. The new 1H-pyrrolo[3,4-c]pyridine-3,6(2H,5H)-dione- and thiazole-containing compound 10 provides new insights regarding the biosynthetic pathway to (pre)colibactin and establishes foundations for future investigation of the intriguing (pre)colibactin structures and its modes of action. | - |
dc.language | eng | - |
dc.relation.ispartof | ChemBioChem | - |
dc.subject | Escherichia coli | - |
dc.subject | heterologous expression | - |
dc.subject | precolibactin | - |
dc.subject | prodrug mechanism | - |
dc.subject | colibactin | - |
dc.title | Critical Intermediates Reveal New Biosynthetic Events in the Enigmatic Colibactin Pathway | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/cbic.201500239 | - |
dc.identifier.pmid | 26052818 | - |
dc.identifier.scopus | eid_2-s2.0-84938741118 | - |
dc.identifier.volume | 16 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 1715 | - |
dc.identifier.epage | 1719 | - |
dc.identifier.eissn | 1439-7633 | - |
dc.identifier.isi | WOS:000359314400003 | - |
dc.identifier.issnl | 1439-4227 | - |