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Conference Paper: Relationship between the secondary structure of the peptide based siRNA carrier and effective gene silencing effect on lung epithelial cells

TitleRelationship between the secondary structure of the peptide based siRNA carrier and effective gene silencing effect on lung epithelial cells
Authors
Issue Date2019
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at https://home.liebertpub.com/publications/journal-of-aerosol-medicine-brand-pulmonary-drug-delivery/24/overview
Citation
The Aerosol Society Drug Delivery to the Lungs Conference (DDL), Edinburgh, Scotland, UK, 12-14 December 2018. Abstracts in Journal of Aerosol Medicine and Pulmonary Drug Delivery, 2019, v. 32 n. 2, p. A16-A17, abstract no. 49 How to Cite?
AbstractPulmonary delivery of small interfering RNA (siRNA) has potential in treating many lung diseases. KL4 is a synthetic surfactant peptide that was initially designed to imitate the function of surfactant protein B (SP-B). The safety and the cationic nature of the KL4 make it an attractive candidate for siRNA delivery. Previous data showed that KL4 peptide could mediate efficient gene silencing effect of siRNA in human lung epithelial cells without signs of cytotoxicity at concentrations used for transfection. However, one of the problems associated with KL4 is poor aqueous solubility. To overcome this problem, five KL4-modified peptides were introduced by replacing leucine with other less hydrophobic amino acid residues such as valine and alanine. The siRNA binding affinity of these modified peptides was examined using a gel retardation assay, and the transfection efficiency was evaluated on A549 cells using siRNA targeting Glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The secondary structure of the modified peptides was investigated by circular dichroism (CD). The results showed that only the KL4 peptide which adopted an alpha helix structure could transfect the siRNA into the cells and mediate an efficient gene silencing effect.
DescriptionPoster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/274195
ISSN
2020 Impact Factor: 2.849
2020 SCImago Journal Rankings: 0.678

 

DC FieldValueLanguage
dc.contributor.authorQiu, Y-
dc.contributor.authorTam, B-
dc.contributor.authorChung, WYW-
dc.contributor.authorMason, AJ-
dc.contributor.authorLam, JKW-
dc.date.accessioned2019-08-18T14:57:01Z-
dc.date.available2019-08-18T14:57:01Z-
dc.date.issued2019-
dc.identifier.citationThe Aerosol Society Drug Delivery to the Lungs Conference (DDL), Edinburgh, Scotland, UK, 12-14 December 2018. Abstracts in Journal of Aerosol Medicine and Pulmonary Drug Delivery, 2019, v. 32 n. 2, p. A16-A17, abstract no. 49-
dc.identifier.issn1941-2711-
dc.identifier.urihttp://hdl.handle.net/10722/274195-
dc.descriptionPoster Presentation-
dc.description.abstractPulmonary delivery of small interfering RNA (siRNA) has potential in treating many lung diseases. KL4 is a synthetic surfactant peptide that was initially designed to imitate the function of surfactant protein B (SP-B). The safety and the cationic nature of the KL4 make it an attractive candidate for siRNA delivery. Previous data showed that KL4 peptide could mediate efficient gene silencing effect of siRNA in human lung epithelial cells without signs of cytotoxicity at concentrations used for transfection. However, one of the problems associated with KL4 is poor aqueous solubility. To overcome this problem, five KL4-modified peptides were introduced by replacing leucine with other less hydrophobic amino acid residues such as valine and alanine. The siRNA binding affinity of these modified peptides was examined using a gel retardation assay, and the transfection efficiency was evaluated on A549 cells using siRNA targeting Glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The secondary structure of the modified peptides was investigated by circular dichroism (CD). The results showed that only the KL4 peptide which adopted an alpha helix structure could transfect the siRNA into the cells and mediate an efficient gene silencing effect.-
dc.languageeng-
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at https://home.liebertpub.com/publications/journal-of-aerosol-medicine-brand-pulmonary-drug-delivery/24/overview-
dc.relation.ispartofJournal of Aerosol Medicine and Pulmonary Drug Delivery-
dc.relation.ispartofThe Drug Delivery to the Lungs Conference (DDL) 29-
dc.rightsJournal of Aerosol Medicine and Pulmonary Drug Delivery. Copyright © Mary Ann Liebert, Inc. Publishers.-
dc.titleRelationship between the secondary structure of the peptide based siRNA carrier and effective gene silencing effect on lung epithelial cells-
dc.typeConference_Paper-
dc.identifier.emailLam, JKW: jkwlam@hku.hk-
dc.identifier.authorityLam, JKW=rp01346-
dc.identifier.hkuros302154-
dc.identifier.volume32-
dc.identifier.issue2-
dc.identifier.spageA16-
dc.identifier.epageA17-
dc.publisher.placeUnited States-
dc.identifier.issnl1941-2711-

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