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Article: Point centromere activity requires an optimal level of centromeric noncoding RNA

TitlePoint centromere activity requires an optimal level of centromeric noncoding RNA
Authors
KeywordsCentromere-binding factor Cbf1
Centromeric transcription
Chromosome instability
Histone H2A variant Htz1
Long noncoding RNA
Issue Date2019
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings of the National Academy of Sciences, 2019, v. 116 n. 13, p. 6270-6279 How to Cite?
AbstractIn budding yeast, which possesses simple point centromeres, we discovered that all of its centromeres express long noncoding RNAs (cenRNAs), especially in S phase. Induction of cenRNAs coincides with CENP-ACse4 loading time and is dependent on DNA replication. Centromeric transcription is repressed by centromere-binding factor Cbf1 and histone H2A variant H2A.ZHtz1 Deletion of CBF1 and H2A.Z HTZ1 results in an up-regulation of cenRNAs; an increased loss of a minichromosome; elevated aneuploidy; a down-regulation of the protein levels of centromeric proteins CENP-ACse4, CENP-A chaperone HJURPScm3, CENP-CMif2, SurvivinBir1, and INCENPSli15; and a reduced chromatin localization of CENP-ACse4, CENP-CMif2, and Aurora BIpl1 When the RNA interference system was introduced to knock down all cenRNAs from the endogenous chromosomes, but not the cenRNA from the circular minichromosome, an increase in minichromosome loss was still observed, suggesting that cenRNA functions in trans to regulate centromere activity. CenRNA knockdown partially alleviates minichromosome loss in cbf1Δ, htz1Δ, and cbf1Δ htz1Δ in a dose-dependent manner, demonstrating that cenRNA level is tightly regulated to epigenetically control point centromere function.
Persistent Identifierhttp://hdl.handle.net/10722/274288
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLing, YH-
dc.contributor.authorYuen, KWY-
dc.date.accessioned2019-08-18T14:58:47Z-
dc.date.available2019-08-18T14:58:47Z-
dc.date.issued2019-
dc.identifier.citationProceedings of the National Academy of Sciences, 2019, v. 116 n. 13, p. 6270-6279-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/274288-
dc.description.abstractIn budding yeast, which possesses simple point centromeres, we discovered that all of its centromeres express long noncoding RNAs (cenRNAs), especially in S phase. Induction of cenRNAs coincides with CENP-ACse4 loading time and is dependent on DNA replication. Centromeric transcription is repressed by centromere-binding factor Cbf1 and histone H2A variant H2A.ZHtz1 Deletion of CBF1 and H2A.Z HTZ1 results in an up-regulation of cenRNAs; an increased loss of a minichromosome; elevated aneuploidy; a down-regulation of the protein levels of centromeric proteins CENP-ACse4, CENP-A chaperone HJURPScm3, CENP-CMif2, SurvivinBir1, and INCENPSli15; and a reduced chromatin localization of CENP-ACse4, CENP-CMif2, and Aurora BIpl1 When the RNA interference system was introduced to knock down all cenRNAs from the endogenous chromosomes, but not the cenRNA from the circular minichromosome, an increase in minichromosome loss was still observed, suggesting that cenRNA functions in trans to regulate centromere activity. CenRNA knockdown partially alleviates minichromosome loss in cbf1Δ, htz1Δ, and cbf1Δ htz1Δ in a dose-dependent manner, demonstrating that cenRNA level is tightly regulated to epigenetically control point centromere function.-
dc.languageeng-
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org-
dc.relation.ispartofProceedings of the National Academy of Sciences-
dc.rightsProceedings of the National Academy of Sciences. Copyright © National Academy of Sciences.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCentromere-binding factor Cbf1-
dc.subjectCentromeric transcription-
dc.subjectChromosome instability-
dc.subjectHistone H2A variant Htz1-
dc.subjectLong noncoding RNA-
dc.titlePoint centromere activity requires an optimal level of centromeric noncoding RNA-
dc.typeArticle-
dc.identifier.emailLing, YH: yhling@hku.hk-
dc.identifier.emailYuen, KWY: kwyyuen@hku.hk-
dc.identifier.authorityYuen, KWY=rp01512-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1073/pnas.1821384116-
dc.identifier.pmid30850541-
dc.identifier.scopuseid_2-s2.0-85063956596-
dc.identifier.hkuros302020-
dc.identifier.volume116-
dc.identifier.issue13-
dc.identifier.spage6270-
dc.identifier.epage6279-
dc.identifier.isiWOS:000462382800069-
dc.publisher.placeUnited States-
dc.identifier.f1000735291633-
dc.identifier.issnl0027-8424-

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