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Conference Paper: Effect of cigarette smoke on airway inflammation and mucus hypersecretion in submerged and well-differentiated airway epithelial cells

TitleEffect of cigarette smoke on airway inflammation and mucus hypersecretion in submerged and well-differentiated airway epithelial cells
Authors
Issue Date2019
PublisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/
Citation
24th Medical Research Conference, Department of Medicine, the University of Hong Kong, Hong Kong, 19 January 2019. In Hong Kong Medical Journal, 2019, v. 25 n. 1, Suppl. 1, p. 11, abstract no. 5 How to Cite?
AbstractIntroduction: Cigarette smoke (CS) is one of the major risk factors for the development of chronic obstructive pulmonary disease. To understand the pathogenesis and mechanism of CS-induced airway injury, submerged culture is widely implemented as a model for primary human airway epithelial cell research. However, a more effective and reliable model should be developed to replicate the characteristics of in vivo airway epithelia. We aimed to compare CS-induced inflammation and mucus hypersecretion in submerged and well-differentiated airway epithelial cells. Methods: Primary normal human bronchial epithelial cells were cultured in either submerged condition in growth factor–supplemented medium to sub-confluence or differentiated at the air-liquid interface (ALI) for 28 days. The CS medium was directly applied to submerged culture or at the apical side of well-differentiated normal human bronchial epithelial cells for 24 hours (n=4). The expression of interleukin (IL)-6, IL-8, and MUC5AC were assessed by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay. Results: The basal secretions of both IL-6 and IL-8 were significantly greater in the supernatant of the basolateral side of the ALI culture than in the submerged culture (IL6: 812.5 ± 105.0 pg/mL vs 100.7 ± 7.5 pg/mL, P<0.001; IL-8: 28 955.0 ± 672.0 pg/mL vs 111.8 ± 10.2 pg/mL, P<0.001). Both IL-6 and IL-8 mRNA and protein were upregulated after CS medium exposure in both submerged and ALI culture. The CS medium also caused upregulation of MUC5AC mRNA in a dose-dependent manner with higher expression in ALI culture compared with submerged culture, in line with the observation of MUC5AC mucin elevation in ALI culture only. Conclusion: Although CS induces airway inflammation and mucus hypersecretion in both submerged and ALI culture, ALI culture more closely resembles the in vivo environment with more serious inflammation and mucus secretion.
Persistent Identifierhttp://hdl.handle.net/10722/275311
ISSN
2020 Impact Factor: 2.227
2020 SCImago Journal Rankings: 0.357

 

DC FieldValueLanguage
dc.contributor.authorChen, R-
dc.contributor.authorIp, MSM-
dc.contributor.authorMak, JCW-
dc.date.accessioned2019-09-10T02:39:58Z-
dc.date.available2019-09-10T02:39:58Z-
dc.date.issued2019-
dc.identifier.citation24th Medical Research Conference, Department of Medicine, the University of Hong Kong, Hong Kong, 19 January 2019. In Hong Kong Medical Journal, 2019, v. 25 n. 1, Suppl. 1, p. 11, abstract no. 5-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/275311-
dc.description.abstractIntroduction: Cigarette smoke (CS) is one of the major risk factors for the development of chronic obstructive pulmonary disease. To understand the pathogenesis and mechanism of CS-induced airway injury, submerged culture is widely implemented as a model for primary human airway epithelial cell research. However, a more effective and reliable model should be developed to replicate the characteristics of in vivo airway epithelia. We aimed to compare CS-induced inflammation and mucus hypersecretion in submerged and well-differentiated airway epithelial cells. Methods: Primary normal human bronchial epithelial cells were cultured in either submerged condition in growth factor–supplemented medium to sub-confluence or differentiated at the air-liquid interface (ALI) for 28 days. The CS medium was directly applied to submerged culture or at the apical side of well-differentiated normal human bronchial epithelial cells for 24 hours (n=4). The expression of interleukin (IL)-6, IL-8, and MUC5AC were assessed by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay. Results: The basal secretions of both IL-6 and IL-8 were significantly greater in the supernatant of the basolateral side of the ALI culture than in the submerged culture (IL6: 812.5 ± 105.0 pg/mL vs 100.7 ± 7.5 pg/mL, P<0.001; IL-8: 28 955.0 ± 672.0 pg/mL vs 111.8 ± 10.2 pg/mL, P<0.001). Both IL-6 and IL-8 mRNA and protein were upregulated after CS medium exposure in both submerged and ALI culture. The CS medium also caused upregulation of MUC5AC mRNA in a dose-dependent manner with higher expression in ALI culture compared with submerged culture, in line with the observation of MUC5AC mucin elevation in ALI culture only. Conclusion: Although CS induces airway inflammation and mucus hypersecretion in both submerged and ALI culture, ALI culture more closely resembles the in vivo environment with more serious inflammation and mucus secretion.-
dc.languageeng-
dc.publisherHong Kong Academy of Medicine Press. The Journal's web site is located at http://www.hkmj.org/-
dc.relation.ispartof24th Medical Research Conference, Department of Medicine, the University of Hong Kong-
dc.relation.ispartofHong Kong Medical Journal-
dc.rightsHong Kong Medical Journal. Copyright © Hong Kong Academy of Medicine Press.-
dc.titleEffect of cigarette smoke on airway inflammation and mucus hypersecretion in submerged and well-differentiated airway epithelial cells-
dc.typeConference_Paper-
dc.identifier.emailIp, MSM: msmip@hku.hk-
dc.identifier.emailMak, JCW: judithmak@hku.hk-
dc.identifier.authorityIp, MSM=rp00347-
dc.identifier.authorityMak, JCW=rp00352-
dc.identifier.hkuros303432-
dc.identifier.volume25-
dc.identifier.issue1, Suppl. 1-
dc.identifier.spage11, abstract no. 5-
dc.identifier.epage11, abstract no. 5-
dc.publisher.placeHong Kong-
dc.identifier.issnl1024-2708-

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