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Conference Paper: Elucidating the localization of centromeric protein a (cenp-a) and the effect of germline and embryonic transcription on cenp-a localization in single cells in holocentric c. elegans
Title | Elucidating the localization of centromeric protein a (cenp-a) and the effect of germline and embryonic transcription on cenp-a localization in single cells in holocentric c. elegans |
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Authors | |
Issue Date | 2019 |
Citation | Cell Symposia: Single Cells: Technology to Biology, Singapore, 24-26 February 2019 How to Cite? |
Abstract | Centromeres are the genomic locus that direct chromosome segregation during cell division. In most eukaryotes, centromere is defined epigenetically by the presence of the centromere-specific histone H3 variant CENP-A, interspaced with H3 nucleosomes. CENP-A nucleates assembly of the kinetochore, a multi-protein complex that binds to microtubules to orchestrate chromosome movement. In C. elegans holocentric chromosomes, centromeric nucleosomes are not occupying the whole genome, but only the poleward-facing mitotic chromatin, as a band. The cross-linked chromatin immunoprecipitation analysis from millions of embryo population shows that CENP-A is enriched in 50% of the genome, but CENP-A protein quantification in nuclei shows that CENP-A is only available to occupy at most 4% of the genome in individual cells. The exact, fewer locations of CENP-A binding in single cells may have been occluded by large population chIP studies. Previous study also show that CENP-A generally anti-correlates with germline and embryonic transcription based on population CENP-A and RNA Pol II chIP-chip and RNA-seq analyses. However, some CENP-A-enriched sites do exhibit transcription in late embryonic stages, while some germline-only or early embryonic gene regions do not have CENP-A binding throughout embryonic development. These findings complicate the understanding of the relationship between transcription and CENP-A localization. To delineate the exact pattern of CENP-A binding in single cell, we have constructed a transgenic DNA methylation enzyme fused with CENP-A to analyze methylated DNA marks in proximity to CENP-A binding in individual cell (DamID). This approach will reduce the averaging effects and enable us to examine the cell-to-cell variation, and variations due to developmental stages and transcription pattern changes. |
Description | Poster Session 2 - no. P.036 |
Persistent Identifier | http://hdl.handle.net/10722/275504 |
DC Field | Value | Language |
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dc.contributor.author | Zhu, J | - |
dc.contributor.author | Yuen, KWY | - |
dc.date.accessioned | 2019-09-10T02:43:52Z | - |
dc.date.available | 2019-09-10T02:43:52Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Cell Symposia: Single Cells: Technology to Biology, Singapore, 24-26 February 2019 | - |
dc.identifier.uri | http://hdl.handle.net/10722/275504 | - |
dc.description | Poster Session 2 - no. P.036 | - |
dc.description.abstract | Centromeres are the genomic locus that direct chromosome segregation during cell division. In most eukaryotes, centromere is defined epigenetically by the presence of the centromere-specific histone H3 variant CENP-A, interspaced with H3 nucleosomes. CENP-A nucleates assembly of the kinetochore, a multi-protein complex that binds to microtubules to orchestrate chromosome movement. In C. elegans holocentric chromosomes, centromeric nucleosomes are not occupying the whole genome, but only the poleward-facing mitotic chromatin, as a band. The cross-linked chromatin immunoprecipitation analysis from millions of embryo population shows that CENP-A is enriched in 50% of the genome, but CENP-A protein quantification in nuclei shows that CENP-A is only available to occupy at most 4% of the genome in individual cells. The exact, fewer locations of CENP-A binding in single cells may have been occluded by large population chIP studies. Previous study also show that CENP-A generally anti-correlates with germline and embryonic transcription based on population CENP-A and RNA Pol II chIP-chip and RNA-seq analyses. However, some CENP-A-enriched sites do exhibit transcription in late embryonic stages, while some germline-only or early embryonic gene regions do not have CENP-A binding throughout embryonic development. These findings complicate the understanding of the relationship between transcription and CENP-A localization. To delineate the exact pattern of CENP-A binding in single cell, we have constructed a transgenic DNA methylation enzyme fused with CENP-A to analyze methylated DNA marks in proximity to CENP-A binding in individual cell (DamID). This approach will reduce the averaging effects and enable us to examine the cell-to-cell variation, and variations due to developmental stages and transcription pattern changes. | - |
dc.language | eng | - |
dc.relation.ispartof | Cell Symposia: Single Cells: Technology to Biology | - |
dc.title | Elucidating the localization of centromeric protein a (cenp-a) and the effect of germline and embryonic transcription on cenp-a localization in single cells in holocentric c. elegans | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Zhu, J: pjingzhu@hku.hk | - |
dc.identifier.email | Yuen, KWY: kwyyuen@hku.hk | - |
dc.identifier.authority | Yuen, KWY=rp01512 | - |
dc.identifier.hkuros | 304134 | - |