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Article: Tropism of influenza B viruses in human respiratory tract explants and airway organoids

TitleTropism of influenza B viruses in human respiratory tract explants and airway organoids
Authors
Issue Date2019
PublisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.com
Citation
European Respiratory Journal, 2019, v. 54 n. 2, article no. 1900008 How to Cite?
AbstractDespite causing regular seasonal epidemics with substantial morbidity, mortality and socioeconomic burden, there is still a lack of research into influenza B viruses (IBVs). In this study, we provide for the first time a systematic investigation on the tropism, replication kinetics and pathogenesis of IBVs in the human respiratory tract. Physiologically relevant ex vivo explant cultures of human bronchus and lung, human airway organoids, and in vitro cultures of differentiated primary human bronchial epithelial cells and type-I-like alveolar epithelial cells were used to study the cellular and tissue tropism, replication competence and induced innate immune response of 16 IBV strains isolated from 1940 to 2012 in comparison with human seasonal influenza A viruses (IAVs), H1N1 and H3N2. IBVs from the diverged Yamagata- and Victoria-like lineages and the earlier undiverged period were included. The majority of IBVs replicated productively in human bronchus and lung with similar competence to seasonal IAVs. IBVs infected a variety of cell types, including ciliated cells, club cells, goblet cells and basal cells, in human airway organoids. Like seasonal IAVs, IBVs are low inducers of pro-inflammatory cytokines and chemokines. Most results suggested a higher preference for the conducting airway than the lower lung and strain-specific rather than lineage-specific pathogenicity of IBVs. Our results highlighted the non-negligible virulence of IBVs which require more attention and further investigation to alleviate the disease burden, especially when treatment options are limited.
Persistent Identifierhttp://hdl.handle.net/10722/276148
ISSN
2020 Impact Factor: 16.671
2020 SCImago Journal Rankings: 4.021
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBui, CHT-
dc.contributor.authorChan, RWY-
dc.contributor.authorNG, MMT-
dc.contributor.authorCheung, MC-
dc.contributor.authorNg, KC-
dc.contributor.authorChan, MPK-
dc.contributor.authorChan, LLY-
dc.contributor.authorFong, JHM-
dc.contributor.authorNicholls, JM-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorChan, MCW-
dc.date.accessioned2019-09-10T02:56:56Z-
dc.date.available2019-09-10T02:56:56Z-
dc.date.issued2019-
dc.identifier.citationEuropean Respiratory Journal, 2019, v. 54 n. 2, article no. 1900008-
dc.identifier.issn0903-1936-
dc.identifier.urihttp://hdl.handle.net/10722/276148-
dc.description.abstractDespite causing regular seasonal epidemics with substantial morbidity, mortality and socioeconomic burden, there is still a lack of research into influenza B viruses (IBVs). In this study, we provide for the first time a systematic investigation on the tropism, replication kinetics and pathogenesis of IBVs in the human respiratory tract. Physiologically relevant ex vivo explant cultures of human bronchus and lung, human airway organoids, and in vitro cultures of differentiated primary human bronchial epithelial cells and type-I-like alveolar epithelial cells were used to study the cellular and tissue tropism, replication competence and induced innate immune response of 16 IBV strains isolated from 1940 to 2012 in comparison with human seasonal influenza A viruses (IAVs), H1N1 and H3N2. IBVs from the diverged Yamagata- and Victoria-like lineages and the earlier undiverged period were included. The majority of IBVs replicated productively in human bronchus and lung with similar competence to seasonal IAVs. IBVs infected a variety of cell types, including ciliated cells, club cells, goblet cells and basal cells, in human airway organoids. Like seasonal IAVs, IBVs are low inducers of pro-inflammatory cytokines and chemokines. Most results suggested a higher preference for the conducting airway than the lower lung and strain-specific rather than lineage-specific pathogenicity of IBVs. Our results highlighted the non-negligible virulence of IBVs which require more attention and further investigation to alleviate the disease burden, especially when treatment options are limited.-
dc.languageeng-
dc.publisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.com-
dc.relation.ispartofEuropean Respiratory Journal-
dc.rightsThis is an author-submitted, peer-reviewed version of a manuscript that has been accepted for publication in the European Respiratory Journal, prior to copy-editing, formatting and typesetting. This version of the manuscript may not be duplicated or reproduced without prior permission from the copyright owner, the European Respiratory Society. The publisher is not responsible or liable for any errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final, copy-edited, published article, which is the version of record, is available without a subscription 18 months after the date of issue publication.-
dc.titleTropism of influenza B viruses in human respiratory tract explants and airway organoids-
dc.typeArticle-
dc.identifier.emailBui, CHT: clyyee@hku.hk-
dc.identifier.emailCheung, MC: bccmc@hku.hk-
dc.identifier.emailNg, KC: kckachun@hku.hk-
dc.identifier.emailNicholls, JM: jmnichol@hkucc.hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.emailChan, MCW: mchan@hku.hk-
dc.identifier.authorityNicholls, JM=rp00364-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.identifier.authorityChan, MCW=rp00420-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1183/13993003.00008-2019-
dc.identifier.pmid31097520-
dc.identifier.scopuseid_2-s2.0-85071353674-
dc.identifier.hkuros304299-
dc.identifier.volume54-
dc.identifier.issue2-
dc.identifier.spagearticle no. 1900008-
dc.identifier.epagearticle no. 1900008-
dc.identifier.isiWOS:000482752200010-
dc.publisher.placeSwitzerland-
dc.identifier.issnl0903-1936-

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