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Conference Paper: The influence of electrical current strength on dentine mineralization
Title | The influence of electrical current strength on dentine mineralization |
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Authors | |
Issue Date | 2019 |
Publisher | International Association for Dental Research. The Abstracts' web site is located at http://www.iadr.org/ |
Citation | The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec Iss A, abstract ID 2043 How to Cite? |
Abstract | Objectives: The aim of this study was to investigate the influence of electrical current strength on dentine mineralization via cooperating electrophoresis with casein phosphopeptide-amorphous calcium phosphate (CPP-ACP).
Methods: Dentine slices with a thickness of 2 mm were prepared by using a diamond saw to perpendicularly slice teeth along their longitudinal axis and polished with a 2000-grit silicon carbide paper under running water.Twenty-one polished dentine slices without cracks or hypo-mineralization were collected and acid-etched with 37% phosphoric acid for 60s to obtain demineralized dentine surface.
For investigating the influence of electrical current strength on dentine mineralization, demineralized dentine slices were randomly allocated into experimental group A (n=6), experimental group B (n=6), experimental group C (n=6) and blank control group (n=3). Samples in experimental groups were remineralized with electrophoresis-aided CPP-ACP mineralization system for 8h (group A treated with 2.0 mA, group B treated with 1.0 mA and group C treated with 0.5 mA). Samples in the blank control group were received no treatment.
After remineralization, all samples were characterized by scanning electron microscope (SEM).
Results: In demineralized dentine slices, the dentine collagen fibers were demineralized, and collagen matrix was exposed (Fig. 1A). After 8 hours mineralization in experimental groups, demineralized dentine collagen matrix was completely remineralized and fully covered with intrafibrillar and interfibrillar crystals (Fig. 1B, C and D). Densely packed crystals were homogeneously distributed along the collagen fibers and imbedded in the spaces between demineralized dentine collagen fibers.
The morphology of newly formed crystals varies with different current strength. It was detected that nanosphere-like crystals, sheet-like crystals and ribbon-like crystals were respectively formed in group A (Fig. 1B), group B (Fig. 1C) and group C (Fig. 1D).
Conclusions: Electrical current strength has the influence on the morphology of newly formed crystals on demineralized dentine |
Description | Poster Session 241 - Dentin: From Formation to Regeneration - Poster Presentation no. 2043 |
Persistent Identifier | http://hdl.handle.net/10722/276346 |
DC Field | Value | Language |
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dc.contributor.author | Zhang, YY | - |
dc.contributor.author | Wong, HM | - |
dc.contributor.author | McGrath, CPJ | - |
dc.contributor.author | Li, QL | - |
dc.date.accessioned | 2019-09-10T03:01:16Z | - |
dc.date.available | 2019-09-10T03:01:16Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec Iss A, abstract ID 2043 | - |
dc.identifier.uri | http://hdl.handle.net/10722/276346 | - |
dc.description | Poster Session 241 - Dentin: From Formation to Regeneration - Poster Presentation no. 2043 | - |
dc.description.abstract | Objectives: The aim of this study was to investigate the influence of electrical current strength on dentine mineralization via cooperating electrophoresis with casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). Methods: Dentine slices with a thickness of 2 mm were prepared by using a diamond saw to perpendicularly slice teeth along their longitudinal axis and polished with a 2000-grit silicon carbide paper under running water.Twenty-one polished dentine slices without cracks or hypo-mineralization were collected and acid-etched with 37% phosphoric acid for 60s to obtain demineralized dentine surface. For investigating the influence of electrical current strength on dentine mineralization, demineralized dentine slices were randomly allocated into experimental group A (n=6), experimental group B (n=6), experimental group C (n=6) and blank control group (n=3). Samples in experimental groups were remineralized with electrophoresis-aided CPP-ACP mineralization system for 8h (group A treated with 2.0 mA, group B treated with 1.0 mA and group C treated with 0.5 mA). Samples in the blank control group were received no treatment. After remineralization, all samples were characterized by scanning electron microscope (SEM). Results: In demineralized dentine slices, the dentine collagen fibers were demineralized, and collagen matrix was exposed (Fig. 1A). After 8 hours mineralization in experimental groups, demineralized dentine collagen matrix was completely remineralized and fully covered with intrafibrillar and interfibrillar crystals (Fig. 1B, C and D). Densely packed crystals were homogeneously distributed along the collagen fibers and imbedded in the spaces between demineralized dentine collagen fibers. The morphology of newly formed crystals varies with different current strength. It was detected that nanosphere-like crystals, sheet-like crystals and ribbon-like crystals were respectively formed in group A (Fig. 1B), group B (Fig. 1C) and group C (Fig. 1D). Conclusions: Electrical current strength has the influence on the morphology of newly formed crystals on demineralized dentine | - |
dc.language | eng | - |
dc.publisher | International Association for Dental Research. The Abstracts' web site is located at http://www.iadr.org/ | - |
dc.relation.ispartof | IADR/AADR/CADR 2019 General Session & Exhibition | - |
dc.relation.ispartof | Journal of Dental Research (Spec Issue) | - |
dc.title | The influence of electrical current strength on dentine mineralization | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Wong, HM: wonghmg@hkucc.hku.hk | - |
dc.identifier.email | McGrath, CPJ: mcgrathc@hkucc.hku.hk | - |
dc.identifier.authority | Wong, HM=rp00042 | - |
dc.identifier.authority | McGrath, CPJ=rp00037 | - |
dc.identifier.hkuros | 302487 | - |
dc.identifier.volume | 98 | - |
dc.identifier.issue | Spec Iss A | - |
dc.identifier.spage | abstract ID 2043 | - |
dc.identifier.epage | abstract ID 2043 | - |
dc.publisher.place | United States | - |