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Article: Incorporation and release of dual growth factors for nerve tissue engineering using nanofibrous bicomponent scaffolds
Title | Incorporation and release of dual growth factors for nerve tissue engineering using nanofibrous bicomponent scaffolds |
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Authors | |
Keywords | bicomponent scaffold dual-source dual-power electrospinning growth factors in vitro degradation in vitro release |
Issue Date | 2018 |
Publisher | Institute of Physics Publishing Ltd. The Journal's web site is located at https://iopscience.iop.org/journal/1748-605X |
Citation | Biomedical Materials, 2018, v. 13 n. 4, p. article no. 044107 How to Cite? |
Abstract | Electrospun fibrous scaffolds have been extensively used as cell-supporting matrices or delivery vehicles for various biomolecules in tissue engineering. Biodegradable scaffolds with tunable degradation behaviors are favorable for various resorbable tissue replacements. In nerve tissue engineering, delivery of growth factors (GFs) such as nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) from scaffolds can be used to promote peripheral nerve repair. In this study, using the established dual-source dual-power electrospinning technique, bicomponent scaffolds incorporated with NGF and GDNF were designed and demonstrated as a strategy to develop scaffolds providing dual GF delivery. NGF and GDNF were encapsulated in poly(D, L-lactic acid) (PDLLA) and poly(lactic-co-glycolic acid) (PLGA) nanofibers, respectively, via emulsion electrospinning. Bicomponent scaffolds with various mass ratios of GDNF/PLGA fibers to NGF/PDLLA fibers were fabricated. Their morphology, structure, properties, and the in vitro degradation were examined. Both types of core–shell structured fibers were evenly distributed in bicomponent scaffolds. Robust scaffolds with varying component ratios were fabricated with average fiber diameter ranging from 307 ± 100 nm to 688 ± 129 nm. The ultimate tensile stress and elastic modulus could be tuned ranging from 0.23 ± 0.07 MPa to 1.41 ± 0.23 MPa, 11.1 ± 3.0 MPa to 75.9 ± 3.3 MPa, respectively. Adjustable degradation was achieved and the weight loss of scaffolds ranged from 9.2% to 44.0% after 42 day degradation test. GDNF and NGF were incorporated with satisfactory encapsulation efficiency and their bioactivity were well preserved. Sustained release of both types of GFs was also achieved. |
Persistent Identifier | http://hdl.handle.net/10722/277118 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 0.712 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | LIU, C | - |
dc.contributor.author | Wang, C | - |
dc.contributor.author | Zhao, Q | - |
dc.contributor.author | Li, X | - |
dc.contributor.author | Xu, F | - |
dc.contributor.author | Yao, X | - |
dc.contributor.author | Wang, M | - |
dc.date.accessioned | 2019-09-20T08:44:47Z | - |
dc.date.available | 2019-09-20T08:44:47Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Biomedical Materials, 2018, v. 13 n. 4, p. article no. 044107 | - |
dc.identifier.issn | 1748-6041 | - |
dc.identifier.uri | http://hdl.handle.net/10722/277118 | - |
dc.description.abstract | Electrospun fibrous scaffolds have been extensively used as cell-supporting matrices or delivery vehicles for various biomolecules in tissue engineering. Biodegradable scaffolds with tunable degradation behaviors are favorable for various resorbable tissue replacements. In nerve tissue engineering, delivery of growth factors (GFs) such as nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) from scaffolds can be used to promote peripheral nerve repair. In this study, using the established dual-source dual-power electrospinning technique, bicomponent scaffolds incorporated with NGF and GDNF were designed and demonstrated as a strategy to develop scaffolds providing dual GF delivery. NGF and GDNF were encapsulated in poly(D, L-lactic acid) (PDLLA) and poly(lactic-co-glycolic acid) (PLGA) nanofibers, respectively, via emulsion electrospinning. Bicomponent scaffolds with various mass ratios of GDNF/PLGA fibers to NGF/PDLLA fibers were fabricated. Their morphology, structure, properties, and the in vitro degradation were examined. Both types of core–shell structured fibers were evenly distributed in bicomponent scaffolds. Robust scaffolds with varying component ratios were fabricated with average fiber diameter ranging from 307 ± 100 nm to 688 ± 129 nm. The ultimate tensile stress and elastic modulus could be tuned ranging from 0.23 ± 0.07 MPa to 1.41 ± 0.23 MPa, 11.1 ± 3.0 MPa to 75.9 ± 3.3 MPa, respectively. Adjustable degradation was achieved and the weight loss of scaffolds ranged from 9.2% to 44.0% after 42 day degradation test. GDNF and NGF were incorporated with satisfactory encapsulation efficiency and their bioactivity were well preserved. Sustained release of both types of GFs was also achieved. | - |
dc.language | eng | - |
dc.publisher | Institute of Physics Publishing Ltd. The Journal's web site is located at https://iopscience.iop.org/journal/1748-605X | - |
dc.relation.ispartof | Biomedical Materials | - |
dc.rights | Biomedical Materials. Copyright © Institute of Physics Publishing Ltd. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | bicomponent scaffold | - |
dc.subject | dual-source dual-power electrospinning | - |
dc.subject | growth factors | - |
dc.subject | in vitro degradation | - |
dc.subject | in vitro release | - |
dc.title | Incorporation and release of dual growth factors for nerve tissue engineering using nanofibrous bicomponent scaffolds | - |
dc.type | Article | - |
dc.identifier.email | Wang, M: memwang@hku.hk | - |
dc.identifier.authority | Wang, M=rp00185 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1088/1748-605X/aab693 | - |
dc.identifier.pmid | 29537390 | - |
dc.identifier.scopus | eid_2-s2.0-85048129902 | - |
dc.identifier.hkuros | 305998 | - |
dc.identifier.volume | 13 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | article no. 044107 | - |
dc.identifier.epage | article no. 044107 | - |
dc.identifier.isi | WOS:000431491500001 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 1748-6041 | - |