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Conference Paper: Apolipoprotein E-deletion potentiates endothelium-dependent relaxations to prostacyclin receptor activation in the mouse aorta
Title | Apolipoprotein E-deletion potentiates endothelium-dependent relaxations to prostacyclin receptor activation in the mouse aorta |
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Authors | |
Issue Date | 2019 |
Citation | 13th International Symposium on Mechanisms of Vasodilatation and 7th International Symposium on Endothelium-Dependent Hyperpolarization (MOVD/EDH 2019), Rotterdam, The Netherlands, 20-22 May 2019 How to Cite? |
Abstract | Introduction: Nitric oxide (NO)-mediated relaxations of isolated arteries are blunted by ageing and high-fat diet, as well as by apolipoprotein E-deletion.
Aims: The present study was designed to examine the prostacyclin receptor (IP)-mediated responses in the mouse aorta and the consequences of apolipoprotein E deletion on IP-mediated responses.
Methods: Wild-type, ApoE-/-, ApoE-/-IP-/- mice (male, five-weeks old) were fed normal chow or high-fat diet during 29 weeks. Aortae were exposed to iloprost (IP receptor agonist), acetylcholine (muscarinic receptor agonist) and UK14304 (α2-adrenergic receptor agonist) and isometric tension was recorded in a Halpern-Mulvany myograph. Protein presences and cyclic nucleotide levels were measured by Western blotting and ELISA, respectively.
Results: Iloprost induced endothelium-, nitric oxide synthase (eNOS)-dependent relaxations in aortae of young wild-type mice by activating IP receptors. Iloprost-induced relaxations were blunted in aortae of mice fed high-fat diet compared to age-matched ones fed normal chow; the blunting was restored by the antagonist of thromboxane-prostanoid (TP) receptors S18886. Apolipoprotein E was present in the plasma and aortae of wild-type (but not of ApoE-/-) mice, especially in the endothelium; its presence was augmented by the high-fat diet. In aortae of young ApoE-/- mice, iloprost-induced relaxations were larger than those in preparations of wild-type mice and were not affected by the high-fat diet. Iloprost did not induce relaxations in aortae of ApoE-/-IP-/- mice. However, acetylcholine- and UK14304-induced relaxations were blunted in the aortae of ApoE-/-IP-/- mice.
Conclusion: Iloprost induces endothelium-, eNOS- and IP-dependent relaxations. High-fat diet favors the activation of TP receptors by iloprost causing contraction. Apolipoprotein E-deletion potentiates relaxations to IP receptor activation irrespective of age and diet. IP deletion blunts NO-mediated relaxations in mouse aorta. |
Description | Poster Session I - no. P01 |
Persistent Identifier | http://hdl.handle.net/10722/277553 |
DC Field | Value | Language |
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dc.contributor.author | Cheng, Y | - |
dc.contributor.author | Dusting, GJ | - |
dc.contributor.author | Vanhoutte, PMGR | - |
dc.contributor.author | Leung, SWS | - |
dc.date.accessioned | 2019-09-20T08:53:15Z | - |
dc.date.available | 2019-09-20T08:53:15Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | 13th International Symposium on Mechanisms of Vasodilatation and 7th International Symposium on Endothelium-Dependent Hyperpolarization (MOVD/EDH 2019), Rotterdam, The Netherlands, 20-22 May 2019 | - |
dc.identifier.uri | http://hdl.handle.net/10722/277553 | - |
dc.description | Poster Session I - no. P01 | - |
dc.description.abstract | Introduction: Nitric oxide (NO)-mediated relaxations of isolated arteries are blunted by ageing and high-fat diet, as well as by apolipoprotein E-deletion. Aims: The present study was designed to examine the prostacyclin receptor (IP)-mediated responses in the mouse aorta and the consequences of apolipoprotein E deletion on IP-mediated responses. Methods: Wild-type, ApoE-/-, ApoE-/-IP-/- mice (male, five-weeks old) were fed normal chow or high-fat diet during 29 weeks. Aortae were exposed to iloprost (IP receptor agonist), acetylcholine (muscarinic receptor agonist) and UK14304 (α2-adrenergic receptor agonist) and isometric tension was recorded in a Halpern-Mulvany myograph. Protein presences and cyclic nucleotide levels were measured by Western blotting and ELISA, respectively. Results: Iloprost induced endothelium-, nitric oxide synthase (eNOS)-dependent relaxations in aortae of young wild-type mice by activating IP receptors. Iloprost-induced relaxations were blunted in aortae of mice fed high-fat diet compared to age-matched ones fed normal chow; the blunting was restored by the antagonist of thromboxane-prostanoid (TP) receptors S18886. Apolipoprotein E was present in the plasma and aortae of wild-type (but not of ApoE-/-) mice, especially in the endothelium; its presence was augmented by the high-fat diet. In aortae of young ApoE-/- mice, iloprost-induced relaxations were larger than those in preparations of wild-type mice and were not affected by the high-fat diet. Iloprost did not induce relaxations in aortae of ApoE-/-IP-/- mice. However, acetylcholine- and UK14304-induced relaxations were blunted in the aortae of ApoE-/-IP-/- mice. Conclusion: Iloprost induces endothelium-, eNOS- and IP-dependent relaxations. High-fat diet favors the activation of TP receptors by iloprost causing contraction. Apolipoprotein E-deletion potentiates relaxations to IP receptor activation irrespective of age and diet. IP deletion blunts NO-mediated relaxations in mouse aorta. | - |
dc.language | eng | - |
dc.relation.ispartof | 13th International Symposium on Mechanisms of Vasodilatation and 7th International Symposium on Endothelium-Dependent Hyperpolarization (MOVD/EDH 2019) | - |
dc.title | Apolipoprotein E-deletion potentiates endothelium-dependent relaxations to prostacyclin receptor activation in the mouse aorta | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Vanhoutte, PMGR: vanhoutt@hku.hk | - |
dc.identifier.email | Leung, SWS: swsleung@hku.hk | - |
dc.identifier.authority | Vanhoutte, PMGR=rp00238 | - |
dc.identifier.authority | Leung, SWS=rp00235 | - |
dc.identifier.hkuros | 305580 | - |
dc.identifier.hkuros | 308348 | - |