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Article: Grass carp prolactin gene: Structural characterization and signal transduction for PACAP-induced prolactin promoter activity
Title | Grass carp prolactin gene: Structural characterization and signal transduction for PACAP-induced prolactin promoter activity |
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Authors | |
Keywords | animal carp drug effect gene expression regulation genetic transcription |
Issue Date | 2018 |
Publisher | Nature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html |
Citation | Scientific Reports, 2018, v. 8 n. 1, p. article no. 4655 How to Cite? |
Abstract | In this study, structural analysis of grass carp prolactin (PRL) gene was performed and the signaling mechanisms for pituitary adenylate cyclase-activating peptide (PACAP) regulation of PRL promoter activity were investigated. In αT3-1 cells, PRL promoter activity could be induced by oPACAP38 which was blocked by PACAP antagonist but not the VIP antagonist. The stimulatory effect of oPACAP38 was mimicked by activation of AC/cAMP and voltage-sensitive Ca2+ channel (VSCC) signaling, or induction of Ca2+ entry. In parallel, PACAP-induced PRL promoter activity was negated or inhibited by suppressing cAMP production, inhibiting PKA activity, removal of extracellular Ca2+, VSCC blockade, calmodulin (CaM) antagonism, and inactivation of CaM kinase II. Similar sensitivity to L-type VSCC, CaM and CaM kinase II inhibition were also observed by substituting cAMP analog for oPACAP38 as the stimulant for PRL promoter activity. Moreover, PACAP-induced PRL promoter activity was also blocked by inhibition of PLC signaling, attenuation of [Ca2+]i immobilization via IP3 receptors, and blockade of PI3K/P70S6K pathway. The PACAP-induced PRL promoter activation may involve transactivation of the transcription factor CREB. These results suggest that PACAP can stimulate PRL promoter activation by PAC1 mediated functional coupling of the Ca2+/CaM/CaM kinase II cascades with the AC/cAMP/PKA pathway. Apparently, other signaling pathways, including PLC/IP3 and PI3K/P70S6K cascades, may also be involved in PACAP induction of PRL gene transcription. |
Persistent Identifier | http://hdl.handle.net/10722/277899 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.900 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Lin, C | - |
dc.contributor.author | Bai, J | - |
dc.contributor.author | He, M | - |
dc.contributor.author | Wong, AOL | - |
dc.date.accessioned | 2019-10-04T08:03:33Z | - |
dc.date.available | 2019-10-04T08:03:33Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Scientific Reports, 2018, v. 8 n. 1, p. article no. 4655 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10722/277899 | - |
dc.description.abstract | In this study, structural analysis of grass carp prolactin (PRL) gene was performed and the signaling mechanisms for pituitary adenylate cyclase-activating peptide (PACAP) regulation of PRL promoter activity were investigated. In αT3-1 cells, PRL promoter activity could be induced by oPACAP38 which was blocked by PACAP antagonist but not the VIP antagonist. The stimulatory effect of oPACAP38 was mimicked by activation of AC/cAMP and voltage-sensitive Ca2+ channel (VSCC) signaling, or induction of Ca2+ entry. In parallel, PACAP-induced PRL promoter activity was negated or inhibited by suppressing cAMP production, inhibiting PKA activity, removal of extracellular Ca2+, VSCC blockade, calmodulin (CaM) antagonism, and inactivation of CaM kinase II. Similar sensitivity to L-type VSCC, CaM and CaM kinase II inhibition were also observed by substituting cAMP analog for oPACAP38 as the stimulant for PRL promoter activity. Moreover, PACAP-induced PRL promoter activity was also blocked by inhibition of PLC signaling, attenuation of [Ca2+]i immobilization via IP3 receptors, and blockade of PI3K/P70S6K pathway. The PACAP-induced PRL promoter activation may involve transactivation of the transcription factor CREB. These results suggest that PACAP can stimulate PRL promoter activation by PAC1 mediated functional coupling of the Ca2+/CaM/CaM kinase II cascades with the AC/cAMP/PKA pathway. Apparently, other signaling pathways, including PLC/IP3 and PI3K/P70S6K cascades, may also be involved in PACAP induction of PRL gene transcription. | - |
dc.language | eng | - |
dc.publisher | Nature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html | - |
dc.relation.ispartof | Scientific Reports | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | animal | - |
dc.subject | carp | - |
dc.subject | drug effect | - |
dc.subject | gene expression regulation | - |
dc.subject | genetic transcription | - |
dc.title | Grass carp prolactin gene: Structural characterization and signal transduction for PACAP-induced prolactin promoter activity | - |
dc.type | Article | - |
dc.identifier.email | He, M: hemu@hkucc.hku.hk | - |
dc.identifier.email | Wong, AOL: olwong@hku.hk | - |
dc.identifier.authority | Wong, AOL=rp00806 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1038/s41598-018-23092-0 | - |
dc.identifier.pmid | 29545542 | - |
dc.identifier.pmcid | PMC5854708 | - |
dc.identifier.scopus | eid_2-s2.0-85044176827 | - |
dc.identifier.hkuros | 306668 | - |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | article no. 4655 | - |
dc.identifier.epage | article no. 4655 | - |
dc.identifier.isi | WOS:000427468500001 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 2045-2322 | - |