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- Publisher Website: 10.1021/acsami.8b20580
- Scopus: eid_2-s2.0-85062370277
- PMID: 30720276
- WOS: WOS:000460996900093
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Article: Spatial Distribution of Biomaterial Microenvironment pH and Its Modulatory Effect on Osteoclasts at the Early Stage of Bone Defect Regeneration
Title | Spatial Distribution of Biomaterial Microenvironment pH and Its Modulatory Effect on Osteoclasts at the Early Stage of Bone Defect Regeneration |
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Authors | |
Keywords | microenvironment boundary interfacial pH osteoclast biodegradable material bone regeneration |
Issue Date | 2019 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/aamick |
Citation | ACS Applied Materials & Interfaces, 2019, v. 11 n. 9, p. 9557-9572 How to Cite? |
Abstract | It is generally accepted that biodegradable materials greatly influence the nearby microenvironment where cells reside; however, the range of interfacial properties has seldom been discussed due to technical bottlenecks. This study aims to depict biomaterial microenvironment boundaries by correlating interfacial H+ distribution with surrounding cell behaviors. Using a disuse-related osteoporotic mouse model, we confirmed that the abnormal activated osteoclasts could be suppressed under relatively alkaline conditions. The differentiation and apatite-resorption capability of osteoclasts were “switched off” when cultured in titrated material extracts with pH values higher than 7.8. To generate a localized alkaline microenvironment, a series of borosilicates were fabricated and their interfacial H+ distributions were monitored spatiotemporally by employing noninvasive microtest technology. By correlating interfacial H+ distribution with osteoclast “switch on/off” behavior, the microenvironment boundary of the tested material was found to be 400 ± 50 μm, which is broader than the generally accepted value, 300 μm. Furthermore, osteoporotic mice implanted with materials with higher interfacial pH values and boarder effective ranges had lower osteoclast activities and a thicker new bone. To conclude, effective proton microenvironment boundaries of degradable biomaterials were depicted and a weak alkaline microenvironment was shown to promote regeneration of osteoporotic bones possibly by suppressing abnormal activated osteoclasts. |
Persistent Identifier | http://hdl.handle.net/10722/278940 |
ISSN | 2023 Impact Factor: 8.3 2023 SCImago Journal Rankings: 2.058 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | LIU, W | - |
dc.contributor.author | DAN, X | - |
dc.contributor.author | Lu, WW | - |
dc.contributor.author | ZHAO, X | - |
dc.contributor.author | RUAN, C | - |
dc.contributor.author | WANG, T | - |
dc.contributor.author | CUI, X | - |
dc.contributor.author | ZHAI, X | - |
dc.contributor.author | MA, Y | - |
dc.contributor.author | WANG, DP | - |
dc.contributor.author | HUANG, W | - |
dc.contributor.author | PAN, H | - |
dc.date.accessioned | 2019-10-21T02:16:44Z | - |
dc.date.available | 2019-10-21T02:16:44Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | ACS Applied Materials & Interfaces, 2019, v. 11 n. 9, p. 9557-9572 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.uri | http://hdl.handle.net/10722/278940 | - |
dc.description.abstract | It is generally accepted that biodegradable materials greatly influence the nearby microenvironment where cells reside; however, the range of interfacial properties has seldom been discussed due to technical bottlenecks. This study aims to depict biomaterial microenvironment boundaries by correlating interfacial H+ distribution with surrounding cell behaviors. Using a disuse-related osteoporotic mouse model, we confirmed that the abnormal activated osteoclasts could be suppressed under relatively alkaline conditions. The differentiation and apatite-resorption capability of osteoclasts were “switched off” when cultured in titrated material extracts with pH values higher than 7.8. To generate a localized alkaline microenvironment, a series of borosilicates were fabricated and their interfacial H+ distributions were monitored spatiotemporally by employing noninvasive microtest technology. By correlating interfacial H+ distribution with osteoclast “switch on/off” behavior, the microenvironment boundary of the tested material was found to be 400 ± 50 μm, which is broader than the generally accepted value, 300 μm. Furthermore, osteoporotic mice implanted with materials with higher interfacial pH values and boarder effective ranges had lower osteoclast activities and a thicker new bone. To conclude, effective proton microenvironment boundaries of degradable biomaterials were depicted and a weak alkaline microenvironment was shown to promote regeneration of osteoporotic bones possibly by suppressing abnormal activated osteoclasts. | - |
dc.language | eng | - |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/aamick | - |
dc.relation.ispartof | ACS Applied Materials & Interfaces | - |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html]. | - |
dc.subject | microenvironment boundary | - |
dc.subject | interfacial pH | - |
dc.subject | osteoclast | - |
dc.subject | biodegradable material | - |
dc.subject | bone regeneration | - |
dc.title | Spatial Distribution of Biomaterial Microenvironment pH and Its Modulatory Effect on Osteoclasts at the Early Stage of Bone Defect Regeneration | - |
dc.type | Article | - |
dc.identifier.email | Lu, WW: wwlu@hku.hk | - |
dc.identifier.authority | Lu, WW=rp00411 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1021/acsami.8b20580 | - |
dc.identifier.pmid | 30720276 | - |
dc.identifier.scopus | eid_2-s2.0-85062370277 | - |
dc.identifier.hkuros | 307539 | - |
dc.identifier.volume | 11 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 9557 | - |
dc.identifier.epage | 9572 | - |
dc.identifier.isi | WOS:000460996900093 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1944-8244 | - |