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Article: A novel regulatory circuit to control indole biosynthesis protects Escherichia coli from nitrosative damages during the anaerobic respiration of nitrate

TitleA novel regulatory circuit to control indole biosynthesis protects Escherichia coli from nitrosative damages during the anaerobic respiration of nitrate
Authors
Keywordsanaerobic growth
Escherichia coli
gene expression regulation
genetics
metabolism
Issue Date2017
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/WileyCDA/WileyTitle/productCd-EMI.html
Citation
Environmental Microbiology, 2017, v. 19 n. 2, p. 598-610 How to Cite?
AbstractIndole is a widely distributed microbial secondary metabolite. It mediates a broad range of physiological processes in both its producing and surrounding species. Yet, indole biosynthesis during the anaerobiosis of bacteria remains largely uncharacterized. Here, we find that while indole production is promoted during fermentation and anaerobic respiration of fumarate and trimethylamine N‐oxide in E. coli, its biosynthesis is repressed during anaerobic respiration of nitrate especially during exponential growth. We show that expression of the indole biosynthetic operon tnaCAB is repressed under this condition by the two component systems NarXL and NarPQ in the global regulator FNR dependent manner. During stationary growth phase of nitrate respiration, indole biosynthesis is derepressed. However, cellular indole concentration remains low. We demonstrate that this is due to the rapid conversion of indole into mutagenic indole nitrosative derivatives under this condition. Consistent with this, a supplement of exogenous indole during nitrate respiration causes elevated mutation frequencies in E. coli cells lacking the detoxifying efflux genes mdtEF, and ectopic over‐expression of tnaAB genes decreases the fitness of E. coli to this physiological condition. Together, these results suggest that indole production is tuned to the bioenergetics activities of E. coli to facilitate its adaptation and fitness.
Persistent Identifierhttp://hdl.handle.net/10722/280277
ISSN
2023 Impact Factor: 4.3
2023 SCImago Journal Rankings: 1.342
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLAI, Y-
dc.contributor.authorXU, Z-
dc.contributor.authorYan, A-
dc.date.accessioned2020-01-21T11:51:06Z-
dc.date.available2020-01-21T11:51:06Z-
dc.date.issued2017-
dc.identifier.citationEnvironmental Microbiology, 2017, v. 19 n. 2, p. 598-610-
dc.identifier.issn1462-2912-
dc.identifier.urihttp://hdl.handle.net/10722/280277-
dc.description.abstractIndole is a widely distributed microbial secondary metabolite. It mediates a broad range of physiological processes in both its producing and surrounding species. Yet, indole biosynthesis during the anaerobiosis of bacteria remains largely uncharacterized. Here, we find that while indole production is promoted during fermentation and anaerobic respiration of fumarate and trimethylamine N‐oxide in E. coli, its biosynthesis is repressed during anaerobic respiration of nitrate especially during exponential growth. We show that expression of the indole biosynthetic operon tnaCAB is repressed under this condition by the two component systems NarXL and NarPQ in the global regulator FNR dependent manner. During stationary growth phase of nitrate respiration, indole biosynthesis is derepressed. However, cellular indole concentration remains low. We demonstrate that this is due to the rapid conversion of indole into mutagenic indole nitrosative derivatives under this condition. Consistent with this, a supplement of exogenous indole during nitrate respiration causes elevated mutation frequencies in E. coli cells lacking the detoxifying efflux genes mdtEF, and ectopic over‐expression of tnaAB genes decreases the fitness of E. coli to this physiological condition. Together, these results suggest that indole production is tuned to the bioenergetics activities of E. coli to facilitate its adaptation and fitness.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/WileyCDA/WileyTitle/productCd-EMI.html-
dc.relation.ispartofEnvironmental Microbiology-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Postprint This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectanaerobic growth-
dc.subjectEscherichia coli-
dc.subjectgene expression regulation-
dc.subjectgenetics-
dc.subjectmetabolism-
dc.titleA novel regulatory circuit to control indole biosynthesis protects Escherichia coli from nitrosative damages during the anaerobic respiration of nitrate-
dc.typeArticle-
dc.identifier.emailYan, A: ayan8@hku.hk-
dc.identifier.authorityYan, A=rp00823-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/1462-2920.13527-
dc.identifier.pmid27631134-
dc.identifier.scopuseid_2-s2.0-84997327881-
dc.identifier.hkuros308988-
dc.identifier.volume19-
dc.identifier.issue2-
dc.identifier.spage598-
dc.identifier.epage610-
dc.identifier.isiWOS:000394973000020-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1462-2912-

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