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Article: In vitro cytocompatibility and osteogenic potential of calcium silicate-based dental cements in a root canal-filling model

TitleIn vitro cytocompatibility and osteogenic potential of calcium silicate-based dental cements in a root canal-filling model
Authors
KeywordsCytocompatibility
periodontal ligament stem cell
root canal
calcium silicate
osteogenic
Issue Date2020
PublisherSAGE Publications (UK and US): Open Access Titles. The Journal's web site is located at http://www.sagepub.com/journals/Journal202169?siteId=sage-us&prodTypes=any&q=The%5CJournal+of+International+Medical+Research&fs=1
Citation
Journal of International Medical Research, 2020, v. 48 n. 4, article no. 300060519894801 How to Cite?
AbstractObjective To investigate the in vitro cytocompatibility and osteogenic potential of an experimental calcium silicate-based cement and the inflammatory response in human periodontal ligament stem cells (hPDLSCs). Methods Cellular responses, osteogenic-related gene expression, and the production of inflammatory cytokines including interleukin (IL)-6 and IL-8 were studied in hPDLSCs exposed to the experimental root canal-filling material C-Root, the commercial tricalcium silicate-based material BioRoot RCS, and the epoxy resin-based material AH Plus. Differences were analyzed using one-way ANOVA with Bonferroni-adjusted pairwise comparison. Results Exposure to BioRoot and C-Root caused time-dependent increases in cell proliferation. Significantly more mineralized nodules were formed in cells exposed to AH Plus and BioRoot compared with the negative control. Alkaline phosphatase (ALP) activity was significantly lower in AH Plus cells compared with negative control, BioRoot, and C-Root cells. ALP, osteocalcin (OCN), and runt-related transcription factor2 (RUNX2) mRNA expression levels were all significantly higher in C-Root compared with AH Plus cells at day 7. IL-6 and IL-8 levels differed significantly among the experimental groups, with the highest IL-8 levels in BioRoot cells at days 7 and 14. Conclusion The experimental root canal-filling material C-Root has similar in vitro cytocompatibility to BioRoot and better osteogenic potential than AH Plus.
Persistent Identifierhttp://hdl.handle.net/10722/281783
ISSN
2023 Impact Factor: 1.4
2023 SCImago Journal Rankings: 0.445
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorJing, Y-
dc.contributor.authorGONG, T-
dc.contributor.authorDuan, CG-
dc.contributor.authorWang, H-
dc.contributor.authorZhang, CF-
dc.contributor.authorNeelakantan, P-
dc.date.accessioned2020-03-27T04:22:29Z-
dc.date.available2020-03-27T04:22:29Z-
dc.date.issued2020-
dc.identifier.citationJournal of International Medical Research, 2020, v. 48 n. 4, article no. 300060519894801-
dc.identifier.issn0300-0605-
dc.identifier.urihttp://hdl.handle.net/10722/281783-
dc.description.abstractObjective To investigate the in vitro cytocompatibility and osteogenic potential of an experimental calcium silicate-based cement and the inflammatory response in human periodontal ligament stem cells (hPDLSCs). Methods Cellular responses, osteogenic-related gene expression, and the production of inflammatory cytokines including interleukin (IL)-6 and IL-8 were studied in hPDLSCs exposed to the experimental root canal-filling material C-Root, the commercial tricalcium silicate-based material BioRoot RCS, and the epoxy resin-based material AH Plus. Differences were analyzed using one-way ANOVA with Bonferroni-adjusted pairwise comparison. Results Exposure to BioRoot and C-Root caused time-dependent increases in cell proliferation. Significantly more mineralized nodules were formed in cells exposed to AH Plus and BioRoot compared with the negative control. Alkaline phosphatase (ALP) activity was significantly lower in AH Plus cells compared with negative control, BioRoot, and C-Root cells. ALP, osteocalcin (OCN), and runt-related transcription factor2 (RUNX2) mRNA expression levels were all significantly higher in C-Root compared with AH Plus cells at day 7. IL-6 and IL-8 levels differed significantly among the experimental groups, with the highest IL-8 levels in BioRoot cells at days 7 and 14. Conclusion The experimental root canal-filling material C-Root has similar in vitro cytocompatibility to BioRoot and better osteogenic potential than AH Plus.-
dc.languageeng-
dc.publisherSAGE Publications (UK and US): Open Access Titles. The Journal's web site is located at http://www.sagepub.com/journals/Journal202169?siteId=sage-us&prodTypes=any&q=The%5CJournal+of+International+Medical+Research&fs=1-
dc.relation.ispartofJournal of International Medical Research-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCytocompatibility-
dc.subjectperiodontal ligament stem cell-
dc.subjectroot canal-
dc.subjectcalcium silicate-
dc.subjectosteogenic-
dc.titleIn vitro cytocompatibility and osteogenic potential of calcium silicate-based dental cements in a root canal-filling model-
dc.typeArticle-
dc.identifier.emailZhang, CF: zhangcf@hku.hk-
dc.identifier.emailNeelakantan, P: prasanna@hku.hk-
dc.identifier.authorityZhang, CF=rp01408-
dc.identifier.authorityNeelakantan, P=rp02214-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1177/0300060519894801-
dc.identifier.pmid31884862-
dc.identifier.pmcidPMC7645323-
dc.identifier.scopuseid_2-s2.0-85094825198-
dc.identifier.hkuros309498-
dc.identifier.volume48-
dc.identifier.issue4-
dc.identifier.spagearticle no. 300060519894801-
dc.identifier.epagearticle no. 300060519894801-
dc.identifier.isiWOS:000507743100001-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl0300-0605-

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