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Article: Tsukamurella asaccharolytica sp. nov., Tsukamurella conjunctivitidis sp. nov. and Tsukamurella sputi sp. nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong

TitleTsukamurella asaccharolytica sp. nov., Tsukamurella conjunctivitidis sp. nov. and Tsukamurella sputi sp. nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong
Authors
KeywordsNovel
Tsukamurella
bacteraemia
conjunctivitis
respiratory infection
Issue Date2020
PublisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org
Citation
International Journal of Systematic and Evolutionary Microbiology, 2020, v. 70 n. 2, p. 995-1006 How to Cite?
AbstractThree bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong. The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species. 16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively. DNA–DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA–DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2–40.9 % Genome-to-Genome Distance Calculator and 86.3–88.9 % average nucleotide identity values]. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella . The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively. Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp. nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp. nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp. nov.
DescriptionLink to Free access
Persistent Identifierhttp://hdl.handle.net/10722/281893
ISSN
2020 Impact Factor: 2.747
2015 SCImago Journal Rankings: 1.088
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTeng, JLL-
dc.contributor.authorFONG, JYH-
dc.contributor.authorFok, KMN-
dc.contributor.authorLEE, HH-
dc.contributor.authorChiu, TH-
dc.contributor.authorTANG, Y-
dc.contributor.authorNgan, AHY-
dc.contributor.authorWong, SSY-
dc.contributor.authorQue, T-L-
dc.contributor.authorLau, SKP-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2020-04-03T07:23:16Z-
dc.date.available2020-04-03T07:23:16Z-
dc.date.issued2020-
dc.identifier.citationInternational Journal of Systematic and Evolutionary Microbiology, 2020, v. 70 n. 2, p. 995-1006-
dc.identifier.issn1466-5026-
dc.identifier.urihttp://hdl.handle.net/10722/281893-
dc.descriptionLink to Free access-
dc.description.abstractThree bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong. The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species. 16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively. DNA–DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA–DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2–40.9 % Genome-to-Genome Distance Calculator and 86.3–88.9 % average nucleotide identity values]. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella . The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively. Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp. nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp. nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp. nov.-
dc.languageeng-
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org-
dc.relation.ispartofInternational Journal of Systematic and Evolutionary Microbiology-
dc.rightsInternational Journal of Systematic and Evolutionary Microbiology. Copyright © Society for General Microbiology.-
dc.rightsThis is an author manuscript that has been accepted for publication in [Journal Title], copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in [Journal Title]. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at [Journal URL], and is freely available without a subscription.-
dc.subjectNovel-
dc.subjectTsukamurella-
dc.subjectbacteraemia-
dc.subjectconjunctivitis-
dc.subjectrespiratory infection-
dc.titleTsukamurella asaccharolytica sp. nov., Tsukamurella conjunctivitidis sp. nov. and Tsukamurella sputi sp. nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong-
dc.typeArticle-
dc.identifier.emailTeng, JLL: llteng@hku.hk-
dc.identifier.emailFok, KMN: kennymnf@hku.hk-
dc.identifier.emailNgan, AHY: nganhy@hku.hk-
dc.identifier.emailWong, SSY: samsonsy@hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityTeng, JLL=rp00277-
dc.identifier.authorityWong, SSY=rp00395-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1099/ijsem.0.003861-
dc.identifier.pmid31738158-
dc.identifier.scopuseid_2-s2.0-85081131310-
dc.identifier.hkuros309651-
dc.identifier.volume70-
dc.identifier.issue2-
dc.identifier.spage995-
dc.identifier.epage1006-
dc.identifier.isiWOS:000522888400042-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1466-5026-

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