File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Interpreting the Pharmacological Mechanisms of Huachansu Capsules on Hepatocellular Carcinoma Through Combining Network Pharmacology and Experimental Evaluation

TitleInterpreting the Pharmacological Mechanisms of Huachansu Capsules on Hepatocellular Carcinoma Through Combining Network Pharmacology and Experimental Evaluation
Authors
KeywordsHuachansu capsules
network pharmacology
hepatocellular carcinoma
molecular targets
KEGG pathway
Issue Date2020
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/pharmacology
Citation
Frontiers in Pharmacology, 2020, v. 11, p. article no. 414 How to Cite?
AbstractHepatocellular carcinoma (HCC) is one of the most fatal cancers across the world. Chinese medicine has been used as adjunctive or complementary therapy for the management of HCC. Huachansu belongs to a class of toxic steroids isolated from toad venom that has important anti-cancer property. This study was aimed to identify the bioactive constituents and molecular targets of Huachansu capsules (HCSCs) for treating HCC using network pharmacology analysis and experimental assays. The major bioactive components of HCSCs were determined using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A series of network pharmacology methods including target prediction, pathway identification, and network establishment were applied to identify the modes of action of HCSCs against HCC. Furthermore, a series of experiments, including MTT, clonogenic assay, 3-D transwell, wound healing assay, as well as flow cytometry, were conducted to verify the inhibitory ability of HCSCs on HCC in vitro. The results showed that 11 chemical components were identified from HCSCs. The network pharmacological analysis showed that there were 82 related anti-HCC targets and 14 potential pathways for these 11 components. Moreover, experimental assays confirmed the inhibitory effects of HCSCs against HCC in vitro. Taken together, our study revealed the synergistic effects of HCSCs on a systematic level, and suggested that HCSCs exhibited anti-HCC effects in a multi-component, multi-target, and multi-pathway manner.
Persistent Identifierhttp://hdl.handle.net/10722/282547
ISSN
2020 Impact Factor: 5.81
2015 SCImago Journal Rankings: 1.845
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHuang, J-
dc.contributor.authorCHEN, F-
dc.contributor.authorZhong, Z-
dc.contributor.authorTan, HY-
dc.contributor.authorWang, N-
dc.contributor.authorLiu, Y-
dc.contributor.authorFang, X-
dc.contributor.authorYang, T-
dc.contributor.authorFeng, Y-
dc.date.accessioned2020-05-15T05:29:31Z-
dc.date.available2020-05-15T05:29:31Z-
dc.date.issued2020-
dc.identifier.citationFrontiers in Pharmacology, 2020, v. 11, p. article no. 414-
dc.identifier.issn1663-9812-
dc.identifier.urihttp://hdl.handle.net/10722/282547-
dc.description.abstractHepatocellular carcinoma (HCC) is one of the most fatal cancers across the world. Chinese medicine has been used as adjunctive or complementary therapy for the management of HCC. Huachansu belongs to a class of toxic steroids isolated from toad venom that has important anti-cancer property. This study was aimed to identify the bioactive constituents and molecular targets of Huachansu capsules (HCSCs) for treating HCC using network pharmacology analysis and experimental assays. The major bioactive components of HCSCs were determined using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A series of network pharmacology methods including target prediction, pathway identification, and network establishment were applied to identify the modes of action of HCSCs against HCC. Furthermore, a series of experiments, including MTT, clonogenic assay, 3-D transwell, wound healing assay, as well as flow cytometry, were conducted to verify the inhibitory ability of HCSCs on HCC in vitro. The results showed that 11 chemical components were identified from HCSCs. The network pharmacological analysis showed that there were 82 related anti-HCC targets and 14 potential pathways for these 11 components. Moreover, experimental assays confirmed the inhibitory effects of HCSCs against HCC in vitro. Taken together, our study revealed the synergistic effects of HCSCs on a systematic level, and suggested that HCSCs exhibited anti-HCC effects in a multi-component, multi-target, and multi-pathway manner.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/pharmacology-
dc.relation.ispartofFrontiers in Pharmacology-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectHuachansu capsules-
dc.subjectnetwork pharmacology-
dc.subjecthepatocellular carcinoma-
dc.subjectmolecular targets-
dc.subjectKEGG pathway-
dc.titleInterpreting the Pharmacological Mechanisms of Huachansu Capsules on Hepatocellular Carcinoma Through Combining Network Pharmacology and Experimental Evaluation-
dc.typeArticle-
dc.identifier.emailZhong, Z: zfzhong@hku.hk-
dc.identifier.emailTan, HY: hyhtan@hku.hk-
dc.identifier.emailWang, N: ckwang@hku.hk-
dc.identifier.emailFeng, Y: yfeng@hku.hk-
dc.identifier.authorityWang, N=rp02075-
dc.identifier.authorityFeng, Y=rp00466-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fphar.2020.00414-
dc.identifier.scopuseid_2-s2.0-85083507803-
dc.identifier.hkuros309973-
dc.identifier.volume11-
dc.identifier.spagearticle no. 414-
dc.identifier.epagearticle no. 414-
dc.identifier.isiWOS:000528719300001-
dc.publisher.placeSwitzerland-
dc.identifier.issnl1663-9812-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats