Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1155/2019/5715180
- Scopus: eid_2-s2.0-85074240118
- PMID: 31687393
- WOS: WOS:000492952300005
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Evaluation of RealStar® Alpha Herpesvirus PCR Kit for Detection of HSV-1, HSV-2, and VZV in Clinical Specimens
Title | Evaluation of RealStar® Alpha Herpesvirus PCR Kit for Detection of HSV-1, HSV-2, and VZV in Clinical Specimens |
---|---|
Authors | |
Keywords | controlled study Herpes simplex virus 2 Herpesviridae Human alphaherpesvirus 1 multiplex real time polymerase chain reaction |
Issue Date | 2019 |
Publisher | Hindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.html |
Citation | BioMed Research International, 2019, v. 2019, p. article no. 5715180 How to Cite? |
Abstract | Several commercial PCR kits are available for detection of herpes simplex virus (HSV) and varicella zoster virus (VZV), but the test performance of one CE-marked in vitro diagnostic kit—RealStar® alpha Herpesvirus PCR Kit—has not been well studied. This study evaluated the performance of RealStar® alpha Herpesvirus PCR Kit 1.0 on the LightCycler® 480 Instrument II for detection and differentiation of HSV-1, HSV-2, and VZV in human clinical specimens. We evaluated the analytical sensitivity of the RealStar® and in-house multiplex real-time PCR assays using serial dilutions of nucleic acids extracted from clinical specimens. The analytical sensitivity of the RealStar® assay was 10, 32, and 100 copies/reaction for HSV-1, HSV-2, and VZV, respectively, which was slightly higher than that of the in-house multiplex real-time PCR assay. Reproducibility of the cycle threshold (Cp) values for each viral target was satisfactory with the intra- and interassay coefficient of variation values below 5% for both assays. One-hundred and fifty-three clinical specimens and 15 proficiency testing samples were used to evaluate the diagnostic performance of RealStar® alpha Herpesvirus PCR Kit against the in-house multiplex real-time PCR assay. The RealStar® assay showed 100% sensitivity and specificity when compared to the in-house assay. Cp values of the RealStar® and in-house assays showed excellent correlation. RealStar® alpha Herpesvirus PCR is a sensitive, specific, and reliable assay for the detection of HSV-1, HSV-2, and VZV, with less extensive verification requirements compared to a laboratory developed assay. |
Persistent Identifier | http://hdl.handle.net/10722/284103 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.656 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yip, CY | - |
dc.contributor.author | Sridhar, S | - |
dc.contributor.author | Leung, KH | - |
dc.contributor.author | Cheng, AKW | - |
dc.contributor.author | Chan, KH | - |
dc.contributor.author | Chan, JFW | - |
dc.contributor.author | Cheng, VCC | - |
dc.contributor.author | Yuen, KY | - |
dc.date.accessioned | 2020-07-20T05:56:07Z | - |
dc.date.available | 2020-07-20T05:56:07Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | BioMed Research International, 2019, v. 2019, p. article no. 5715180 | - |
dc.identifier.issn | 2314-6133 | - |
dc.identifier.uri | http://hdl.handle.net/10722/284103 | - |
dc.description.abstract | Several commercial PCR kits are available for detection of herpes simplex virus (HSV) and varicella zoster virus (VZV), but the test performance of one CE-marked in vitro diagnostic kit—RealStar® alpha Herpesvirus PCR Kit—has not been well studied. This study evaluated the performance of RealStar® alpha Herpesvirus PCR Kit 1.0 on the LightCycler® 480 Instrument II for detection and differentiation of HSV-1, HSV-2, and VZV in human clinical specimens. We evaluated the analytical sensitivity of the RealStar® and in-house multiplex real-time PCR assays using serial dilutions of nucleic acids extracted from clinical specimens. The analytical sensitivity of the RealStar® assay was 10, 32, and 100 copies/reaction for HSV-1, HSV-2, and VZV, respectively, which was slightly higher than that of the in-house multiplex real-time PCR assay. Reproducibility of the cycle threshold (Cp) values for each viral target was satisfactory with the intra- and interassay coefficient of variation values below 5% for both assays. One-hundred and fifty-three clinical specimens and 15 proficiency testing samples were used to evaluate the diagnostic performance of RealStar® alpha Herpesvirus PCR Kit against the in-house multiplex real-time PCR assay. The RealStar® assay showed 100% sensitivity and specificity when compared to the in-house assay. Cp values of the RealStar® and in-house assays showed excellent correlation. RealStar® alpha Herpesvirus PCR is a sensitive, specific, and reliable assay for the detection of HSV-1, HSV-2, and VZV, with less extensive verification requirements compared to a laboratory developed assay. | - |
dc.language | eng | - |
dc.publisher | Hindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.html | - |
dc.relation.ispartof | BioMed Research International | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | controlled study | - |
dc.subject | Herpes simplex virus 2 | - |
dc.subject | Herpesviridae | - |
dc.subject | Human alphaherpesvirus 1 | - |
dc.subject | multiplex real time polymerase chain reaction | - |
dc.title | Evaluation of RealStar® Alpha Herpesvirus PCR Kit for Detection of HSV-1, HSV-2, and VZV in Clinical Specimens | - |
dc.type | Article | - |
dc.identifier.email | Yip, CY: yipcyril@hku.hk | - |
dc.identifier.email | Sridhar, S: sid8998@hku.hk | - |
dc.identifier.email | Leung, KH: khl17@hku.hk | - |
dc.identifier.email | Chan, KH: chankh2@hkucc.hku.hk | - |
dc.identifier.email | Chan, JFW: jfwchan@hku.hk | - |
dc.identifier.email | Cheng, VCC: vcccheng@hkucc.hku.hk | - |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | - |
dc.identifier.authority | Yip, CY=rp01721 | - |
dc.identifier.authority | Sridhar, S=rp02249 | - |
dc.identifier.authority | Chan, KH=rp01921 | - |
dc.identifier.authority | Chan, JFW=rp01736 | - |
dc.identifier.authority | Yuen, KY=rp00366 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1155/2019/5715180 | - |
dc.identifier.pmid | 31687393 | - |
dc.identifier.pmcid | PMC6803750 | - |
dc.identifier.scopus | eid_2-s2.0-85074240118 | - |
dc.identifier.hkuros | 310968 | - |
dc.identifier.volume | 2019 | - |
dc.identifier.spage | article no. 5715180 | - |
dc.identifier.epage | article no. 5715180 | - |
dc.identifier.isi | WOS:000492952300005 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 2314-6133 | - |