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- Publisher Website: 10.1038/s41592-020-0883-z
- Scopus: eid_2-s2.0-85087009316
- PMID: 32601425
- WOS: WOS:000544223800004
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Article: Mid-infrared metabolic imaging with vibrational probes
Title | Mid-infrared metabolic imaging with vibrational probes |
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Authors | |
Issue Date | 2020 |
Publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/nmeth |
Citation | Nature Methods, 2020, v. 17, p. 844-851 How to Cite? |
Abstract | Understanding metabolism is indispensable in unraveling the mechanistic basis of many physiological and pathological processes. However, in situ metabolic imaging tools are still lacking. Here we introduce a framework for mid-infrared (MIR) metabolic imaging by coupling the emerging high-information-throughput MIR microscopy with specifically designed IR-active vibrational probes. We present three categories of small vibrational tags including azide bond, 13C-edited carbonyl bond and deuterium-labeled probes to interrogate various metabolic activities in cells, small organisms and mice. Two MIR imaging platforms are implemented including broadband Fourier transform infrared microscopy and discrete frequency infrared microscopy with a newly incorporated spectral region (2,000–2,300 cm−1). Our technique is uniquely suited to metabolic imaging with high information throughput. In particular, we performed single-cell metabolic profiling including heterogeneity characterization, and large-area metabolic imaging at tissue or organ level with rich spectral information. |
Persistent Identifier | http://hdl.handle.net/10722/284996 |
ISSN | 2021 Impact Factor: 47.990 2020 SCImago Journal Rankings: 19.469 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Shi, L | - |
dc.contributor.author | Liu, X | - |
dc.contributor.author | Shi, L | - |
dc.contributor.author | Stinson, HT | - |
dc.contributor.author | Rowlette, J | - |
dc.contributor.author | Kahl, LJ | - |
dc.contributor.author | Evans, CR | - |
dc.contributor.author | Zheng, C | - |
dc.contributor.author | Dietrich, LEP | - |
dc.contributor.author | Min, W | - |
dc.date.accessioned | 2020-08-07T09:05:23Z | - |
dc.date.available | 2020-08-07T09:05:23Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Nature Methods, 2020, v. 17, p. 844-851 | - |
dc.identifier.issn | 1548-7091 | - |
dc.identifier.uri | http://hdl.handle.net/10722/284996 | - |
dc.description.abstract | Understanding metabolism is indispensable in unraveling the mechanistic basis of many physiological and pathological processes. However, in situ metabolic imaging tools are still lacking. Here we introduce a framework for mid-infrared (MIR) metabolic imaging by coupling the emerging high-information-throughput MIR microscopy with specifically designed IR-active vibrational probes. We present three categories of small vibrational tags including azide bond, 13C-edited carbonyl bond and deuterium-labeled probes to interrogate various metabolic activities in cells, small organisms and mice. Two MIR imaging platforms are implemented including broadband Fourier transform infrared microscopy and discrete frequency infrared microscopy with a newly incorporated spectral region (2,000–2,300 cm−1). Our technique is uniquely suited to metabolic imaging with high information throughput. In particular, we performed single-cell metabolic profiling including heterogeneity characterization, and large-area metabolic imaging at tissue or organ level with rich spectral information. | - |
dc.language | eng | - |
dc.publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/nmeth | - |
dc.relation.ispartof | Nature Methods | - |
dc.rights | This is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: https://doi.org/[insert DOI] | - |
dc.title | Mid-infrared metabolic imaging with vibrational probes | - |
dc.type | Article | - |
dc.identifier.email | Zheng, C: cgzheng@hku.hk | - |
dc.identifier.authority | Zheng, C=rp02473 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1038/s41592-020-0883-z | - |
dc.identifier.pmid | 32601425 | - |
dc.identifier.pmcid | PMC7396315 | - |
dc.identifier.scopus | eid_2-s2.0-85087009316 | - |
dc.identifier.hkuros | 312513 | - |
dc.identifier.volume | 17 | - |
dc.identifier.spage | 844 | - |
dc.identifier.epage | 851 | - |
dc.identifier.isi | WOS:000544223800004 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 1548-7091 | - |