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Article: Repurposing the Native Type I-F CRISPR-Cas System in Pseudomonas aeruginosa for Genome Editing

TitleRepurposing the Native Type I-F CRISPR-Cas System in Pseudomonas aeruginosa for Genome Editing
Authors
Xu, ZLI, YYan, A
Issue Date2020
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home
Citation
STAR Protocols, 2020, v. 1 n. 1, p. article no. 100039 How to Cite?
DOI: http://dx.doi.org/10.1016/j.xpro.2020.100039
AbstractSUMMARY: Repurposing the broadly distributed native CRISPR-Cas systems in prokaryotes for genome editing is emerging as a new strategy for genetic manipulations. We recently reported the establishment of a single plasmid-mediated, one-step genome-editing technique in a multidrug-resistant genotype of the opportunistic pathogen Pseudomonas aeruginosa by harnessing its endogenous type I-F CRISPR-Cas system. The platform is readily applicable in additional type I-F CRISPR-containing clinical and environmental P. aeruginosa isolates. Herein, we provide the detailed protocol for the methodology. For complete details on the establishment and exploitation of this protocol, please refer to Xu et al. (2019).
Persistent Identifierhttp://hdl.handle.net/10722/284997
ISSN
2666-1667
2023 Impact Factor: 1.3
2023 SCImago Journal Rankings: 0.955
ISI Accession Number ID
WOS:001050034000003

 

DC FieldValueLanguage
dc.contributor.authorXu, Z-
dc.contributor.authorLI, Y-
dc.contributor.authorYan, A-
dc.date.accessioned2020-08-07T09:05:23Z-
dc.date.available2020-08-07T09:05:23Z-
dc.date.issued2020-
dc.identifier.citationSTAR Protocols, 2020, v. 1 n. 1, p. article no. 100039-
dc.identifier.issn2666-1667-
dc.identifier.urihttp://hdl.handle.net/10722/284997-
dc.description.abstractSUMMARY: Repurposing the broadly distributed native CRISPR-Cas systems in prokaryotes for genome editing is emerging as a new strategy for genetic manipulations. We recently reported the establishment of a single plasmid-mediated, one-step genome-editing technique in a multidrug-resistant genotype of the opportunistic pathogen Pseudomonas aeruginosa by harnessing its endogenous type I-F CRISPR-Cas system. The platform is readily applicable in additional type I-F CRISPR-containing clinical and environmental P. aeruginosa isolates. Herein, we provide the detailed protocol for the methodology. For complete details on the establishment and exploitation of this protocol, please refer to Xu et al. (2019).-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at https://www.cell.com/star-protocols/home-
dc.relation.ispartofSTAR Protocols-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleRepurposing the Native Type I-F CRISPR-Cas System in Pseudomonas aeruginosa for Genome Editing-
dc.typeArticle-
dc.identifier.emailXu, Z: zelingxu@connect.hku.hk-
dc.identifier.emailYan, A: ayan8@hku.hk-
dc.identifier.authorityYan, A=rp00823-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.xpro.2020.100039-
dc.identifier.scopuseid_2-s2.0-85087305292-
dc.identifier.hkuros312522-
dc.identifier.volume1-
dc.identifier.issue1-
dc.identifier.spagearticle no. 100039-
dc.identifier.epagearticle no. 100039-
dc.identifier.isiWOS:001050034000003-
dc.publisher.placeUnited States-
dc.identifier.issnl2666-1667-

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