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Article: Harnessing the type I CRISPR‐Cas systems for genome editing in prokaryotes

TitleHarnessing the type I CRISPR‐Cas systems for genome editing in prokaryotes
Authors
KeywordsCRISPR-cas System
CRISPR-associated Protein
Bacteriophages
Issue Date2020
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/WileyCDA/WileyTitle/productCd-EMI.html
Citation
Environmental Microbiology, 2020, Epub 2020-06-08 How to Cite?
AbstractGenetic analysis is crucial to the understanding, exploitation, and control of microorganisms. The advent of CRISPR‐Cas‐based genome‐editing techniques, particularly those mediated by the single‐effector (Cas9 and Cas12a) class 2 CRISPR‐Cas systems, has revolutionized the genetics in model eukaryotic organisms. However, their applications in prokaryotes are rather limited, largely owing to the exceptional diversity of DNA homeostasis in microorganisms and severe cytotoxicity of overexpressing these nuclease proteins in certain genotypes. Remarkably, CRISPR‐Cas systems belonging to different classes and types are continuously identified in prokaryotic genomes and serve as a deep reservoir for expansion of the CRISPR‐based genetic toolkits. ~90% of the CRISPR‐Cas systems identified so far belong to the class 1 system which hinges on multi‐protein effector complexes for DNA interference. Harnessing these widespread native CRISPR‐Cas systems for ‘built‐in’ genome editing represents an emerging and powerful genetic tool in prokaryotes, especially in the genetically recalcitrant non‐model species and strains. In this progress review, we introduce the general workflow of this emerging editing platform and summarize its establishment in a growing number of prokaryotes by harnessing the most widespread, diverse type I CRISPR‐Cas systems present in their genomes. We also discuss the various factors affecting the success and efficiency of this editing platform and the corresponding solutions.
Persistent Identifierhttp://hdl.handle.net/10722/285124
ISSN
2023 Impact Factor: 4.3
2023 SCImago Journal Rankings: 1.342
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXu, Z-
dc.contributor.authorLI, Y-
dc.contributor.authorLi, M-
dc.contributor.authorXiang, H-
dc.contributor.authorYan, A-
dc.date.accessioned2020-08-07T09:07:04Z-
dc.date.available2020-08-07T09:07:04Z-
dc.date.issued2020-
dc.identifier.citationEnvironmental Microbiology, 2020, Epub 2020-06-08-
dc.identifier.issn1462-2912-
dc.identifier.urihttp://hdl.handle.net/10722/285124-
dc.description.abstractGenetic analysis is crucial to the understanding, exploitation, and control of microorganisms. The advent of CRISPR‐Cas‐based genome‐editing techniques, particularly those mediated by the single‐effector (Cas9 and Cas12a) class 2 CRISPR‐Cas systems, has revolutionized the genetics in model eukaryotic organisms. However, their applications in prokaryotes are rather limited, largely owing to the exceptional diversity of DNA homeostasis in microorganisms and severe cytotoxicity of overexpressing these nuclease proteins in certain genotypes. Remarkably, CRISPR‐Cas systems belonging to different classes and types are continuously identified in prokaryotic genomes and serve as a deep reservoir for expansion of the CRISPR‐based genetic toolkits. ~90% of the CRISPR‐Cas systems identified so far belong to the class 1 system which hinges on multi‐protein effector complexes for DNA interference. Harnessing these widespread native CRISPR‐Cas systems for ‘built‐in’ genome editing represents an emerging and powerful genetic tool in prokaryotes, especially in the genetically recalcitrant non‐model species and strains. In this progress review, we introduce the general workflow of this emerging editing platform and summarize its establishment in a growing number of prokaryotes by harnessing the most widespread, diverse type I CRISPR‐Cas systems present in their genomes. We also discuss the various factors affecting the success and efficiency of this editing platform and the corresponding solutions.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/WileyCDA/WileyTitle/productCd-EMI.html-
dc.relation.ispartofEnvironmental Microbiology-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Postprint This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectCRISPR-cas System-
dc.subjectCRISPR-associated Protein-
dc.subjectBacteriophages-
dc.titleHarnessing the type I CRISPR‐Cas systems for genome editing in prokaryotes-
dc.typeArticle-
dc.identifier.emailXu, Z: zelingxu@connect.hku.hk-
dc.identifier.emailYan, A: ayan8@hku.hk-
dc.identifier.authorityYan, A=rp00823-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/1462-2920.15116-
dc.identifier.scopuseid_2-s2.0-85087306357-
dc.identifier.hkuros312523-
dc.identifier.volumeEpub 2020-06-08-
dc.identifier.isiWOS:000543749700001-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1462-2912-

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