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- Publisher Website: 10.1073/pnas.0404521102
- Scopus: eid_2-s2.0-14844349235
- PMID: 15738413
- WOS: WOS:000227533100052
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Article: Identification of viral genes essential for replication of murine γ-herpesvirus 68 using signature-tagged mutagenesis
Title | Identification of viral genes essential for replication of murine γ-herpesvirus 68 using signature-tagged mutagenesis |
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Authors | |
Keywords | Transposition Deoxyuridine-triphosphatase Bacterial artificial chromosome Herpesvirus Functional mapping |
Issue Date | 2005 |
Citation | Proceedings of the National Academy of Sciences of the United States of America, 2005, v. 102, n. 10, p. 3805-3810 How to Cite? |
Abstract | γ-Herpesviruses, Epstein-Barr virus, and Kaposi's sarcoma-associated herpesvirus are important human pathogens, because they are involved in tumor development. Murine γ-herpesvirus-68 (MHV-68 or γHV-68) has emerged as a small animal model system for the study of γ-herpesvirus pathogenesis and host-virus interactions. To identify the genes required for viral replication in vitro and in vivo, we generated 1,152 mutants using signature-tagged transposon mutagenesis on an infectious bacterial artificial chromosome of MHV-68. Almost every ORF was mutated by random insertion. For each ORF, a mutant with an insertion proximal to the N terminus of each ORF was examined for the ability to grow in fibroblasts. Our results indicate that 41 genes are essential for in vitro growth, whereas 26 are nonessential and 6 attenuated. Replication-competent mutants were pooled to infect mice, which led to the discovery of ORF 54 being important for MHV-68 to replicate in the lung. This genetic analysis of a tumor-associated herpesvirus at the whole genome level validates signature-tagged transposon mutagenesis screening as an effective genetic system to identify important virulent genes in vivo and define interactions with the host immune system. © 2005 by The National Academy of Sciences of the USA. |
Persistent Identifier | http://hdl.handle.net/10722/285587 |
ISSN | 2023 Impact Factor: 9.4 2023 SCImago Journal Rankings: 3.737 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Song, Moon Jung | - |
dc.contributor.author | Hwang, Seungmin | - |
dc.contributor.author | Wong, Wendy H. | - |
dc.contributor.author | Wu, Ting Ting | - |
dc.contributor.author | Lee, Sangmi | - |
dc.contributor.author | Liao, Hsiang I. | - |
dc.contributor.author | Sun, Ren | - |
dc.date.accessioned | 2020-08-18T04:56:08Z | - |
dc.date.available | 2020-08-18T04:56:08Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the United States of America, 2005, v. 102, n. 10, p. 3805-3810 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10722/285587 | - |
dc.description.abstract | γ-Herpesviruses, Epstein-Barr virus, and Kaposi's sarcoma-associated herpesvirus are important human pathogens, because they are involved in tumor development. Murine γ-herpesvirus-68 (MHV-68 or γHV-68) has emerged as a small animal model system for the study of γ-herpesvirus pathogenesis and host-virus interactions. To identify the genes required for viral replication in vitro and in vivo, we generated 1,152 mutants using signature-tagged transposon mutagenesis on an infectious bacterial artificial chromosome of MHV-68. Almost every ORF was mutated by random insertion. For each ORF, a mutant with an insertion proximal to the N terminus of each ORF was examined for the ability to grow in fibroblasts. Our results indicate that 41 genes are essential for in vitro growth, whereas 26 are nonessential and 6 attenuated. Replication-competent mutants were pooled to infect mice, which led to the discovery of ORF 54 being important for MHV-68 to replicate in the lung. This genetic analysis of a tumor-associated herpesvirus at the whole genome level validates signature-tagged transposon mutagenesis screening as an effective genetic system to identify important virulent genes in vivo and define interactions with the host immune system. © 2005 by The National Academy of Sciences of the USA. | - |
dc.language | eng | - |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America | - |
dc.subject | Transposition | - |
dc.subject | Deoxyuridine-triphosphatase | - |
dc.subject | Bacterial artificial chromosome | - |
dc.subject | Herpesvirus | - |
dc.subject | Functional mapping | - |
dc.title | Identification of viral genes essential for replication of murine γ-herpesvirus 68 using signature-tagged mutagenesis | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1073/pnas.0404521102 | - |
dc.identifier.pmid | 15738413 | - |
dc.identifier.pmcid | PMC553290 | - |
dc.identifier.scopus | eid_2-s2.0-14844349235 | - |
dc.identifier.volume | 102 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 3805 | - |
dc.identifier.epage | 3810 | - |
dc.identifier.isi | WOS:000227533100052 | - |
dc.identifier.issnl | 0027-8424 | - |