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Article: Systematic identification of cellular signals reactivating Kaposi sarcoma-associated herpesvirus

TitleSystematic identification of cellular signals reactivating Kaposi sarcoma-associated herpesvirus
Authors
Issue Date2007
Citation
PLoS Pathogens, 2007, v. 3, n. 3, article no. e44 How to Cite?
AbstractThe herpesvirus life cycle has two distinct phases: latency and lytic replication. The balance between these two phases is critical for viral pathogenesis. It is believed that cellular signals regulate the switch from latency to lytic replication. To systematically evaluate the cellular signals regulating this reactivation process in Kaposi sarcoma-associated herpesvirus, the effects of 26,000 full-length cDNA expression constructs on viral reactivation were individually assessed in primary effusion lymphoma-derived cells that harbor the latent virus. A group of diverse cellular signaling proteins were identified and validated in their effect of inducing viral lytic gene expression from the latent viral genome. The results suggest that multiple cellular signaling pathways can reactivate the virus in a genetically homogeneous cell population. Further analysis revealed that the Raf/MEK/ERK/Ets-1 pathway mediates Ras-induced reactivation. The same pathway also mediates spontaneous reactivation, which sets the first example to our knowledge of a specific cellular pathway being studied in the spontaneous reactivation process. Our study provides a functional genomic approach to systematically identify the cellular signals regulating the herpesvirus life cycle, thus facilitating better understanding of a fundamental issue in virology and identifying novel therapeutic targets. © 2007 Yu et al.
Persistent Identifierhttp://hdl.handle.net/10722/285598
ISSN
2023 Impact Factor: 5.5
2023 SCImago Journal Rankings: 2.223
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYu, Fuqu-
dc.contributor.authorHarada, Josephine N.-
dc.contributor.authorBrown, Helen J.-
dc.contributor.authorDeng, Hongyu-
dc.contributor.authorMoon, Jung Song-
dc.contributor.authorWu, Ting Ting-
dc.contributor.authorKato-Stankiewicz, Juran-
dc.contributor.authorNelson, Christian G.-
dc.contributor.authorVieira, Jeffrey-
dc.contributor.authorTamanoi, Fuyuhiko-
dc.contributor.authorChanda, Sumit K.-
dc.contributor.authorSun, Ren-
dc.date.accessioned2020-08-18T04:56:09Z-
dc.date.available2020-08-18T04:56:09Z-
dc.date.issued2007-
dc.identifier.citationPLoS Pathogens, 2007, v. 3, n. 3, article no. e44-
dc.identifier.issn1553-7366-
dc.identifier.urihttp://hdl.handle.net/10722/285598-
dc.description.abstractThe herpesvirus life cycle has two distinct phases: latency and lytic replication. The balance between these two phases is critical for viral pathogenesis. It is believed that cellular signals regulate the switch from latency to lytic replication. To systematically evaluate the cellular signals regulating this reactivation process in Kaposi sarcoma-associated herpesvirus, the effects of 26,000 full-length cDNA expression constructs on viral reactivation were individually assessed in primary effusion lymphoma-derived cells that harbor the latent virus. A group of diverse cellular signaling proteins were identified and validated in their effect of inducing viral lytic gene expression from the latent viral genome. The results suggest that multiple cellular signaling pathways can reactivate the virus in a genetically homogeneous cell population. Further analysis revealed that the Raf/MEK/ERK/Ets-1 pathway mediates Ras-induced reactivation. The same pathway also mediates spontaneous reactivation, which sets the first example to our knowledge of a specific cellular pathway being studied in the spontaneous reactivation process. Our study provides a functional genomic approach to systematically identify the cellular signals regulating the herpesvirus life cycle, thus facilitating better understanding of a fundamental issue in virology and identifying novel therapeutic targets. © 2007 Yu et al.-
dc.languageeng-
dc.relation.ispartofPLoS Pathogens-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleSystematic identification of cellular signals reactivating Kaposi sarcoma-associated herpesvirus-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.ppat.0030044-
dc.identifier.pmid17397260-
dc.identifier.pmcidPMC1839163-
dc.identifier.scopuseid_2-s2.0-34047204739-
dc.identifier.volume3-
dc.identifier.issue3-
dc.identifier.spagearticle no. e44-
dc.identifier.epagearticle no. e44-
dc.identifier.eissn1553-7374-
dc.identifier.isiWOS:000248495200019-
dc.identifier.issnl1553-7366-

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