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Article: Identification and functional characterization of the left origin of lytic replication of murine gammaherpesvirus 68

TitleIdentification and functional characterization of the left origin of lytic replication of murine gammaherpesvirus 68
Authors
KeywordsCCAAT boxes
NF-Y
oriLyt
KSHV
MHV-68
Issue Date2009
Citation
Virology, 2009, v. 387, n. 2, p. 285-295 How to Cite?
AbstractMurine gammaherpesvirus 68 (MHV-68) replicates robustly in cell culture, providing a model for studying viral genome replication during de novo infection of tumor-associated herpesviruses. We have previously identified a 1.25-kb origin of lytic replication (oriLyt) for MHV-68. To further investigate the molecular mechanism of viral genome replication, we first fine-mapped essential cis-elements from this oriLyt fragment using a transposon-mediated high-density mutagenesis method. The result provided information for us to identify a second oriLyt located towards the left end of MHV-68 genome using a de novo infection-replication assay. We further characterized this left oriLyt by scanning deletion analysis and site-directed mutations, and showed that several CCAAT motifs are essential for oriLyt function, whereas an AT-rich region enhances replication. However, GC-rich repeats are not important cis-element. Moreover, we identified a cellular transcription factor, NF-Y, which binds to CCAAT boxes in EMSA and associates with oriLyt in ChIP assay. Using a dominant negative expression plasmid, we demonstrated that NF-Y plays an important role in mediating MHV-68 genome replication during de novo infection. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/285651
ISSN
2023 Impact Factor: 2.8
2023 SCImago Journal Rankings: 0.838
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGong, Danyang-
dc.contributor.authorQi, Jing-
dc.contributor.authorArumugaswami, Vaithilingaraja-
dc.contributor.authorSun, Ren-
dc.contributor.authorDeng, Hongyu-
dc.date.accessioned2020-08-18T04:56:18Z-
dc.date.available2020-08-18T04:56:18Z-
dc.date.issued2009-
dc.identifier.citationVirology, 2009, v. 387, n. 2, p. 285-295-
dc.identifier.issn0042-6822-
dc.identifier.urihttp://hdl.handle.net/10722/285651-
dc.description.abstractMurine gammaherpesvirus 68 (MHV-68) replicates robustly in cell culture, providing a model for studying viral genome replication during de novo infection of tumor-associated herpesviruses. We have previously identified a 1.25-kb origin of lytic replication (oriLyt) for MHV-68. To further investigate the molecular mechanism of viral genome replication, we first fine-mapped essential cis-elements from this oriLyt fragment using a transposon-mediated high-density mutagenesis method. The result provided information for us to identify a second oriLyt located towards the left end of MHV-68 genome using a de novo infection-replication assay. We further characterized this left oriLyt by scanning deletion analysis and site-directed mutations, and showed that several CCAAT motifs are essential for oriLyt function, whereas an AT-rich region enhances replication. However, GC-rich repeats are not important cis-element. Moreover, we identified a cellular transcription factor, NF-Y, which binds to CCAAT boxes in EMSA and associates with oriLyt in ChIP assay. Using a dominant negative expression plasmid, we demonstrated that NF-Y plays an important role in mediating MHV-68 genome replication during de novo infection. © 2009 Elsevier Inc. All rights reserved.-
dc.languageeng-
dc.relation.ispartofVirology-
dc.subjectCCAAT boxes-
dc.subjectNF-Y-
dc.subjectoriLyt-
dc.subjectKSHV-
dc.subjectMHV-68-
dc.titleIdentification and functional characterization of the left origin of lytic replication of murine gammaherpesvirus 68-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.virol.2009.02.029-
dc.identifier.pmid19285330-
dc.identifier.pmcidPMC2715915-
dc.identifier.scopuseid_2-s2.0-64849098120-
dc.identifier.volume387-
dc.identifier.issue2-
dc.identifier.spage285-
dc.identifier.epage295-
dc.identifier.eissn1096-0341-
dc.identifier.isiWOS:000265663100006-
dc.identifier.issnl0042-6822-

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