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Article: Rapid mRNA-display selection of an IL-6 inhibitor using continuous-flow magnetic separation

TitleRapid mRNA-display selection of an IL-6 inhibitor using continuous-flow magnetic separation
Authors
KeywordsmRNA
selection methods
ligand design
directed evolution
antibodies
Issue Date2011
Citation
Angewandte Chemie - International Edition, 2011, v. 50, n. 36, p. 8295-8298 How to Cite?
AbstractEven flow: Microfluidics technology can rapidly generate affinity reagents that bind to protein targets using mRNA display. The continuous-flow magnetic separation (CFMS) method (see scheme; steps a-h) offers reproducible means to impose stringent selection conditions without the need for a specialized device. The method yields approximately a 30-fold greater partition efficiency over conventional methods when using interleukin-6 as a model target. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/285683
ISSN
2023 Impact Factor: 16.1
2023 SCImago Journal Rankings: 5.300
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorOlson, C. Anders-
dc.contributor.authorAdams, Jonathan D.-
dc.contributor.authorTakahashi, Terry T.-
dc.contributor.authorQi, Hangfei-
dc.contributor.authorHowell, Shannon M.-
dc.contributor.authorWu, Ting Ting-
dc.contributor.authorRoberts, Richard W.-
dc.contributor.authorSun, Ren-
dc.contributor.authorSoh, H. Tom-
dc.date.accessioned2020-08-18T04:56:22Z-
dc.date.available2020-08-18T04:56:22Z-
dc.date.issued2011-
dc.identifier.citationAngewandte Chemie - International Edition, 2011, v. 50, n. 36, p. 8295-8298-
dc.identifier.issn1433-7851-
dc.identifier.urihttp://hdl.handle.net/10722/285683-
dc.description.abstractEven flow: Microfluidics technology can rapidly generate affinity reagents that bind to protein targets using mRNA display. The continuous-flow magnetic separation (CFMS) method (see scheme; steps a-h) offers reproducible means to impose stringent selection conditions without the need for a specialized device. The method yields approximately a 30-fold greater partition efficiency over conventional methods when using interleukin-6 as a model target. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.-
dc.languageeng-
dc.relation.ispartofAngewandte Chemie - International Edition-
dc.subjectmRNA-
dc.subjectselection methods-
dc.subjectligand design-
dc.subjectdirected evolution-
dc.subjectantibodies-
dc.titleRapid mRNA-display selection of an IL-6 inhibitor using continuous-flow magnetic separation-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/anie.201101149-
dc.identifier.pmid21761516-
dc.identifier.pmcidPMC3747967-
dc.identifier.scopuseid_2-s2.0-80052079213-
dc.identifier.volume50-
dc.identifier.issue36-
dc.identifier.spage8295-
dc.identifier.epage8298-
dc.identifier.eissn1521-3773-
dc.identifier.isiWOS:000295259700013-
dc.identifier.f100013361052-
dc.identifier.issnl1433-7851-

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