File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Fluorescent imaging of single nanoparticles and viruses on a smart phone

TitleFluorescent imaging of single nanoparticles and viruses on a smart phone
Authors
Keywordssingle virus imaging
fluorescence imaging
cell phone microscopy
single nanoparticle
Issue Date2013
Citation
ACS Nano, 2013, v. 7, n. 10, p. 9147-9155 How to Cite?
AbstractOptical imaging of nanoscale objects, whether it is based on scattering or fluorescence, is a challenging task due to reduced detection signal-to-noise ratio and contrast at subwavelength dimensions. Here, we report a field-portable fluorescence microscopy platform installed on a smart phone for imaging of individual nanoparticles as well as viruses using a lightweight and compact opto-mechanical attachment to the existing camera module of the cell phone. This hand-held fluorescent imaging device utilizes (i) a compact 450 nm laser diode that creates oblique excitation on the sample plane with an incidence angle of ∼75, (ii) a long-pass thin-film interference filter to reject the scattered excitation light, (iii) an external lens creating 2× optical magnification, and (iv) a translation stage for focus adjustment. We tested the imaging performance of this smart-phone-enabled microscopy platform by detecting isolated 100 nm fluorescent particles as well as individual human cytomegaloviruses that are fluorescently labeled. The size of each detected nano-object on the cell phone platform was validated using scanning electron microscopy images of the same samples. This field-portable fluorescence microscopy attachment to the cell phone, weighing only ∼186 g, could be used for specific and sensitive imaging of subwavelength objects including various bacteria and viruses and, therefore, could provide a valuable platform for the practice of nanotechnology in field settings and for conducting viral load measurements and other biomedical tests even in remote and resource-limited environments. © 2013 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/285715
ISSN
2023 Impact Factor: 15.8
2023 SCImago Journal Rankings: 4.593
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWei, Qingshan-
dc.contributor.authorQi, Hangfei-
dc.contributor.authorLuo, Wei-
dc.contributor.authorTseng, Derek-
dc.contributor.authorKi, So Jung-
dc.contributor.authorWan, Zhe-
dc.contributor.authorGöröcs, Zoltán-
dc.contributor.authorBentolila, Laurent A.-
dc.contributor.authorWu, Ting Ting-
dc.contributor.authorSun, Ren-
dc.contributor.authorOzcan, Aydogan-
dc.date.accessioned2020-08-18T04:56:27Z-
dc.date.available2020-08-18T04:56:27Z-
dc.date.issued2013-
dc.identifier.citationACS Nano, 2013, v. 7, n. 10, p. 9147-9155-
dc.identifier.issn1936-0851-
dc.identifier.urihttp://hdl.handle.net/10722/285715-
dc.description.abstractOptical imaging of nanoscale objects, whether it is based on scattering or fluorescence, is a challenging task due to reduced detection signal-to-noise ratio and contrast at subwavelength dimensions. Here, we report a field-portable fluorescence microscopy platform installed on a smart phone for imaging of individual nanoparticles as well as viruses using a lightweight and compact opto-mechanical attachment to the existing camera module of the cell phone. This hand-held fluorescent imaging device utilizes (i) a compact 450 nm laser diode that creates oblique excitation on the sample plane with an incidence angle of ∼75, (ii) a long-pass thin-film interference filter to reject the scattered excitation light, (iii) an external lens creating 2× optical magnification, and (iv) a translation stage for focus adjustment. We tested the imaging performance of this smart-phone-enabled microscopy platform by detecting isolated 100 nm fluorescent particles as well as individual human cytomegaloviruses that are fluorescently labeled. The size of each detected nano-object on the cell phone platform was validated using scanning electron microscopy images of the same samples. This field-portable fluorescence microscopy attachment to the cell phone, weighing only ∼186 g, could be used for specific and sensitive imaging of subwavelength objects including various bacteria and viruses and, therefore, could provide a valuable platform for the practice of nanotechnology in field settings and for conducting viral load measurements and other biomedical tests even in remote and resource-limited environments. © 2013 American Chemical Society.-
dc.languageeng-
dc.relation.ispartofACS Nano-
dc.subjectsingle virus imaging-
dc.subjectfluorescence imaging-
dc.subjectcell phone microscopy-
dc.subjectsingle nanoparticle-
dc.titleFluorescent imaging of single nanoparticles and viruses on a smart phone-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1021/nn4037706-
dc.identifier.pmid24016065-
dc.identifier.pmcidPMC3951925-
dc.identifier.scopuseid_2-s2.0-84886997177-
dc.identifier.volume7-
dc.identifier.issue10-
dc.identifier.spage9147-
dc.identifier.epage9155-
dc.identifier.eissn1936-086X-
dc.identifier.isiWOS:000326209100088-
dc.identifier.issnl1936-0851-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats