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Article: Functional anatomy of the human microprocessor

TitleFunctional anatomy of the human microprocessor
Authors
Issue Date2015
Citation
Cell, 2015, v. 161, n. 6, p. 1374-1387 How to Cite?
Abstract© 2015 Elsevier Inc. MicroRNA (miRNA) maturation is initiated by Microprocessor composed of RNase III DROSHA and its cofactor DGCR8, whose fidelity is critical for generation of functional miRNAs. To understand how Microprocessor recognizes pri-miRNAs, we here reconstitute human Microprocessor with purified recombinant proteins. We find that Microprocessor is an ∼ 364 kDa heterotrimeric complex of one DROSHA and two DGCR8 molecules. Together with a 23-amino acid peptide from DGCR8, DROSHA constitutes a minimal functional core. DROSHA serves as a "ruler" by measuring 11 bp from the basal ssRNA-dsRNA junction. DGCR8 interacts with the stem and apical elements through its dsRNA-binding domains and RNA-binding heme domain, respectively, allowing efficient and accurate processing. DROSHA and DGCR8, respectively, recognize the basal UG and apical UGU motifs, which ensure proper orientation of the complex. These findings clarify controversies over the action mechanism of DROSHA and allow us to build a general model for pri-miRNA processing.
Persistent Identifierhttp://hdl.handle.net/10722/285756
ISSN
2023 Impact Factor: 45.5
2023 SCImago Journal Rankings: 24.342
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNguyen, Tuan Anh-
dc.contributor.authorJo, Myung Hyun-
dc.contributor.authorChoi, Yeon Gil-
dc.contributor.authorPark, Joha-
dc.contributor.authorKwon, S. Chul-
dc.contributor.authorHohng, Sungchul-
dc.contributor.authorKim, V. Narry-
dc.contributor.authorWoo, Jae Sung-
dc.date.accessioned2020-08-18T04:56:33Z-
dc.date.available2020-08-18T04:56:33Z-
dc.date.issued2015-
dc.identifier.citationCell, 2015, v. 161, n. 6, p. 1374-1387-
dc.identifier.issn0092-8674-
dc.identifier.urihttp://hdl.handle.net/10722/285756-
dc.description.abstract© 2015 Elsevier Inc. MicroRNA (miRNA) maturation is initiated by Microprocessor composed of RNase III DROSHA and its cofactor DGCR8, whose fidelity is critical for generation of functional miRNAs. To understand how Microprocessor recognizes pri-miRNAs, we here reconstitute human Microprocessor with purified recombinant proteins. We find that Microprocessor is an ∼ 364 kDa heterotrimeric complex of one DROSHA and two DGCR8 molecules. Together with a 23-amino acid peptide from DGCR8, DROSHA constitutes a minimal functional core. DROSHA serves as a "ruler" by measuring 11 bp from the basal ssRNA-dsRNA junction. DGCR8 interacts with the stem and apical elements through its dsRNA-binding domains and RNA-binding heme domain, respectively, allowing efficient and accurate processing. DROSHA and DGCR8, respectively, recognize the basal UG and apical UGU motifs, which ensure proper orientation of the complex. These findings clarify controversies over the action mechanism of DROSHA and allow us to build a general model for pri-miRNA processing.-
dc.languageeng-
dc.relation.ispartofCell-
dc.titleFunctional anatomy of the human microprocessor-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.cell.2015.05.010-
dc.identifier.pmid26027739-
dc.identifier.scopuseid_2-s2.0-84930478808-
dc.identifier.volume161-
dc.identifier.issue6-
dc.identifier.spage1374-
dc.identifier.epage1387-
dc.identifier.eissn1097-4172-
dc.identifier.isiWOS:000355935000016-
dc.identifier.f1000725527749-
dc.identifier.issnl0092-8674-

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