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- Publisher Website: 10.1128/JVI.78.17.9123-9131.2004
- Scopus: eid_2-s2.0-4143097024
- PMID: 15308708
- WOS: WOS:000223386600017
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Article: Identification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68
Title | Identification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68 |
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Authors | |
Issue Date | 2004 |
Citation | Journal of Virology, 2004, v. 78, n. 17, p. 9123-9131 How to Cite? |
Abstract | Human gammaherpesviruses are associated with lymphomas and other malignancies. Murine gammaherpesvirus 68 (MHV-68) infection of mice has emerged as a model for understanding gammaherpesvirus pathogenesis in vivo. In contrast to human gammaherpesviruses, MHV-68 replicates in permissive cell lines in a robust manner, presenting an efficient model to study the basic mechanisms for DNA replication and recombination processes. In addition, MHV-68 also infects a broad range of cells of different tissue types and from different host species, and the viral genome persists as an episome in infected cells. These features make MHV-68 an attractive system on which to build gene delivery vectors. We have therefore undertaken a study to identify the cis elements required for MHV-68 genome replication and packaging. Here we report that an 8.4-kb MHV-68 genomic fragment between ORF66 and ORF73 conferred on the plasmid the ability to replicate; replication required the presence of either de novo viral infection or viral reactivation from latency. We further mapped the origin of lytic replication (oriLyt) to a 1.25-kb region. Moreover, we demonstrated that the terminal repeat of the viral genome is sufficient for packaging of the replicated oriLyt plasmid into mature viral particles. Functional identification of the MHV-68 oriLyt and packaging signal has laid a foundation for investigating the mechanisms controlling gammaherpesvirus DNA replication during the viral lytic phase and will also serve as a base on which to design gene delivery vectors. |
Persistent Identifier | http://hdl.handle.net/10722/285919 |
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.378 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Deng, Hongyu | - |
dc.contributor.author | Chu, Julia T. | - |
dc.contributor.author | Park, No Hee | - |
dc.contributor.author | Sun, Ren | - |
dc.date.accessioned | 2020-08-18T04:56:59Z | - |
dc.date.available | 2020-08-18T04:56:59Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Journal of Virology, 2004, v. 78, n. 17, p. 9123-9131 | - |
dc.identifier.issn | 0022-538X | - |
dc.identifier.uri | http://hdl.handle.net/10722/285919 | - |
dc.description.abstract | Human gammaherpesviruses are associated with lymphomas and other malignancies. Murine gammaherpesvirus 68 (MHV-68) infection of mice has emerged as a model for understanding gammaherpesvirus pathogenesis in vivo. In contrast to human gammaherpesviruses, MHV-68 replicates in permissive cell lines in a robust manner, presenting an efficient model to study the basic mechanisms for DNA replication and recombination processes. In addition, MHV-68 also infects a broad range of cells of different tissue types and from different host species, and the viral genome persists as an episome in infected cells. These features make MHV-68 an attractive system on which to build gene delivery vectors. We have therefore undertaken a study to identify the cis elements required for MHV-68 genome replication and packaging. Here we report that an 8.4-kb MHV-68 genomic fragment between ORF66 and ORF73 conferred on the plasmid the ability to replicate; replication required the presence of either de novo viral infection or viral reactivation from latency. We further mapped the origin of lytic replication (oriLyt) to a 1.25-kb region. Moreover, we demonstrated that the terminal repeat of the viral genome is sufficient for packaging of the replicated oriLyt plasmid into mature viral particles. Functional identification of the MHV-68 oriLyt and packaging signal has laid a foundation for investigating the mechanisms controlling gammaherpesvirus DNA replication during the viral lytic phase and will also serve as a base on which to design gene delivery vectors. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Virology | - |
dc.title | Identification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68 | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1128/JVI.78.17.9123-9131.2004 | - |
dc.identifier.pmid | 15308708 | - |
dc.identifier.pmcid | PMC506910 | - |
dc.identifier.scopus | eid_2-s2.0-4143097024 | - |
dc.identifier.volume | 78 | - |
dc.identifier.issue | 17 | - |
dc.identifier.spage | 9123 | - |
dc.identifier.epage | 9131 | - |
dc.identifier.isi | WOS:000223386600017 | - |
dc.identifier.issnl | 0022-538X | - |