File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation

TitleA Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation
Authors
Keywordselectroporation
membrane resealing
electrotransfection
live cell staining
cell adhesion
Issue Date2020
PublisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/micromachines
Citation
Micromachines, 2020, v. 11 n. 8, p. article no. 767 How to Cite?
AbstractA novel electroporation system was developed to introduce transient membrane pores to cells in a spatially and temporally controlled manner, allowing us to achieve fast electrotransfection and live cell staining as well as to systematically interrogate the dynamics of the cell membrane. Specifically, using this platform, we showed that both reversible and irreversible electroporation could be induced in the cell population, with nano-sized membrane pores in the former case being able to self-reseal in ~10 min. In addition, green fluorescent protein(GFP)-vinculin plasmid and 543 phalloidin have been delivered successively into fibroblast cells, which enables us to monitor the distinct roles of vinculin and F-actin in cell adhesion and migration as well as their possible interplay during these processes. Compared to conventional bulk electroporation and staining methods, the new system offers advantages such as low-voltage operation, cellular level manipulation and testing, fast and adjustable transfection/staining and real-time monitoring; the new system therefore could be useful in different biophysical studies in the future.
Persistent Identifierhttp://hdl.handle.net/10722/286228
ISSN
2023 Impact Factor: 3.0
2023 SCImago Journal Rankings: 0.549
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZHANG, Y-
dc.contributor.authorYan, Z-
dc.contributor.authorXIA, X-
dc.contributor.authorLin, Y-
dc.date.accessioned2020-08-31T07:00:58Z-
dc.date.available2020-08-31T07:00:58Z-
dc.date.issued2020-
dc.identifier.citationMicromachines, 2020, v. 11 n. 8, p. article no. 767-
dc.identifier.issn2072-666X-
dc.identifier.urihttp://hdl.handle.net/10722/286228-
dc.description.abstractA novel electroporation system was developed to introduce transient membrane pores to cells in a spatially and temporally controlled manner, allowing us to achieve fast electrotransfection and live cell staining as well as to systematically interrogate the dynamics of the cell membrane. Specifically, using this platform, we showed that both reversible and irreversible electroporation could be induced in the cell population, with nano-sized membrane pores in the former case being able to self-reseal in ~10 min. In addition, green fluorescent protein(GFP)-vinculin plasmid and 543 phalloidin have been delivered successively into fibroblast cells, which enables us to monitor the distinct roles of vinculin and F-actin in cell adhesion and migration as well as their possible interplay during these processes. Compared to conventional bulk electroporation and staining methods, the new system offers advantages such as low-voltage operation, cellular level manipulation and testing, fast and adjustable transfection/staining and real-time monitoring; the new system therefore could be useful in different biophysical studies in the future.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/micromachines-
dc.relation.ispartofMicromachines-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectelectroporation-
dc.subjectmembrane resealing-
dc.subjectelectrotransfection-
dc.subjectlive cell staining-
dc.subjectcell adhesion-
dc.titleA Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation-
dc.typeArticle-
dc.identifier.emailLin, Y: ylin@hkucc.hku.hk-
dc.identifier.authorityLin, Y=rp00080-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/mi11080767-
dc.identifier.pmid32796554-
dc.identifier.pmcidPMC7466103-
dc.identifier.scopuseid_2-s2.0-85090026150-
dc.identifier.hkuros313410-
dc.identifier.volume11-
dc.identifier.issue8-
dc.identifier.spagearticle no. 767-
dc.identifier.epagearticle no. 767-
dc.identifier.isiWOS:000579606000001-
dc.publisher.placeSwitzerland-
dc.identifier.issnl2072-666X-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats