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- Publisher Website: 10.1039/C9TB01520E
- Scopus: eid_2-s2.0-85078687497
- PMID: 31829384
- WOS: WOS:000510754400022
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Article: Multifunctional fibrous scaffolds for bone regeneration with enhanced vascularization
Title | Multifunctional fibrous scaffolds for bone regeneration with enhanced vascularization |
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Authors | |
Keywords | Bone Calcium phosphate Cell culture Endothelial cells Mammals |
Issue Date | 2020 |
Publisher | Royal Society of Chemistry. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/tb#!recentarticles&all |
Citation | Journal of Materials Chemistry B, 2020, v. 8 n. 4, p. 636-647 How to Cite? |
Abstract | Due to the structural similarity to the extracellular matrix of human tissue and the ultra-high surface area-to-volume ratio, three dimensional electrospun fibrous structures have been increasingly used as tissue engineering scaffolds. Given that successful bone regeneration requires both good osteogenesis and vascularization, producing scaffolds that have both osteogenic and angiogenic potential is highly desirable. In this investigation, tricomponent fibrous scaffolds simultaneously incorporated with recombinant human vein endothelial growth factor (rhVEGF), recombinant human bone morphogenetic protein-2 (rhBMP-2) and bioactive calcium phosphate (Ca-P) nanoparticles are produced through a novel multi-source multi-power electrospinning method, and sequential growth factor release with a quick rhVEGF release and a steady rhBMP-2 release is achieved. The enhanced human umbilical vein endothelial cell (HUVEC) migration and tube formation, and up-regulated human bone marrow derived mesenchymal stem cell (hBMSC) osteogenic differentiation and mineralization demonstrate that tricomponent scaffolds have balanced angiogenic–osteogenic properties in vitro. 8 weeks after the scaffold implantation into the cranial defects of mice, obvious new bone regeneration and newly formed capillaries are observed in tricomponent scaffolds, suggesting that the tricomponent scaffolds enhance osteogenesis in vivo with required vascularization, which shows the great potential of the tricomponent scaffolds in bone tissue regeneration. |
Persistent Identifier | http://hdl.handle.net/10722/286232 |
ISSN | 2023 Impact Factor: 6.1 2023 SCImago Journal Rankings: 1.216 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wang, C | - |
dc.contributor.author | Lu, WW | - |
dc.contributor.author | Wang, M | - |
dc.date.accessioned | 2020-08-31T07:01:01Z | - |
dc.date.available | 2020-08-31T07:01:01Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Journal of Materials Chemistry B, 2020, v. 8 n. 4, p. 636-647 | - |
dc.identifier.issn | 2050-750X | - |
dc.identifier.uri | http://hdl.handle.net/10722/286232 | - |
dc.description.abstract | Due to the structural similarity to the extracellular matrix of human tissue and the ultra-high surface area-to-volume ratio, three dimensional electrospun fibrous structures have been increasingly used as tissue engineering scaffolds. Given that successful bone regeneration requires both good osteogenesis and vascularization, producing scaffolds that have both osteogenic and angiogenic potential is highly desirable. In this investigation, tricomponent fibrous scaffolds simultaneously incorporated with recombinant human vein endothelial growth factor (rhVEGF), recombinant human bone morphogenetic protein-2 (rhBMP-2) and bioactive calcium phosphate (Ca-P) nanoparticles are produced through a novel multi-source multi-power electrospinning method, and sequential growth factor release with a quick rhVEGF release and a steady rhBMP-2 release is achieved. The enhanced human umbilical vein endothelial cell (HUVEC) migration and tube formation, and up-regulated human bone marrow derived mesenchymal stem cell (hBMSC) osteogenic differentiation and mineralization demonstrate that tricomponent scaffolds have balanced angiogenic–osteogenic properties in vitro. 8 weeks after the scaffold implantation into the cranial defects of mice, obvious new bone regeneration and newly formed capillaries are observed in tricomponent scaffolds, suggesting that the tricomponent scaffolds enhance osteogenesis in vivo with required vascularization, which shows the great potential of the tricomponent scaffolds in bone tissue regeneration. | - |
dc.language | eng | - |
dc.publisher | Royal Society of Chemistry. The Journal's web site is located at http://pubs.rsc.org/en/journals/journalissues/tb#!recentarticles&all | - |
dc.relation.ispartof | Journal of Materials Chemistry B | - |
dc.subject | Bone | - |
dc.subject | Calcium phosphate | - |
dc.subject | Cell culture | - |
dc.subject | Endothelial cells | - |
dc.subject | Mammals | - |
dc.title | Multifunctional fibrous scaffolds for bone regeneration with enhanced vascularization | - |
dc.type | Article | - |
dc.identifier.email | Lu, WW: wwlu@hku.hk | - |
dc.identifier.email | Wang, M: memwang@hku.hk | - |
dc.identifier.authority | Lu, WW=rp00411 | - |
dc.identifier.authority | Wang, M=rp00185 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1039/C9TB01520E | - |
dc.identifier.pmid | 31829384 | - |
dc.identifier.scopus | eid_2-s2.0-85078687497 | - |
dc.identifier.hkuros | 313804 | - |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 636 | - |
dc.identifier.epage | 647 | - |
dc.identifier.isi | WOS:000510754400022 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 2050-750X | - |