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Article: Bcl-2 Overexpression and Hypoxia Synergistically Enhance Angiogenic Properties of Dental Pulp Stem Cells
Title | Bcl-2 Overexpression and Hypoxia Synergistically Enhance Angiogenic Properties of Dental Pulp Stem Cells |
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Authors | |
Keywords | Bcl-2 gene modification angiogenesis post-implantation cell survival vascularization |
Issue Date | 2020 |
Publisher | Molecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms |
Citation | International Journal of Molecular Sciences, 2020, v. 21 n. 17, p. article no. 6159 How to Cite? |
Abstract | Post-implantation cell survival and angio-/vasculogenesis are critical for the success of cell-based regenerative strategies. The current study aimed to overexpress B-cell lymphoma 2 (Bcl-2) gene in dental pulp stem cells (DPSCs) and examine the anti-apoptotic and angio-/vasculogenic effects both in-vitro and in-vivo. DPSCs were transduced with Bcl-2-green fluorescent protein (GFP) lentiviral particles and examined for cell proliferation and apoptosis. The cells were cultured under normoxic or hypoxic (0.5 mM CoCl2) conditions and examined for the expression of angiogenic factors and effects on endothelial cell proliferation, migration and vessel morphogenesis. Cells with or without hypoxic preconditioning were used in in-vivo Matrigel plug assay to study the post-implantation cell survival and angio-/vasculogenesis. Bcl-2-overexpressing-DPSCs showed significantly lower apoptosis than that of null-GFP-DPSCs under serum-free conditions. Under hypoxia, Bcl-2-overexpressing-DPSCs expressed significantly higher levels of vascular endothelial growth factor compared to that under normoxia and null-GFP-DPSCs. Consequently, Bcl-2-overexpressing-DPSCs significantly enhanced endothelial cell proliferation, migration and vascular tube formation on Matrigel. Immunohistological assessment of in-vivo transplanted Matrigel plugs showed significantly higher cell survival and vasculature in hypoxic preconditioned Bcl-2-overexpressing-DPSC group compared to null-GFP-DPSC group. In conclusion, Bcl-2 overexpression and hypoxic-preconditioning could be synergistically used to enhance post-implantation cell survival and angio-/vasculogenic properties of DPSCs. |
Persistent Identifier | http://hdl.handle.net/10722/286620 |
ISSN | 2023 Impact Factor: 4.9 2023 SCImago Journal Rankings: 1.179 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Dissanayaka, WL | - |
dc.contributor.author | HAN, Y | - |
dc.contributor.author | ZHANG, L | - |
dc.contributor.author | Zou, T | - |
dc.contributor.author | Zhang, C | - |
dc.date.accessioned | 2020-09-04T13:28:09Z | - |
dc.date.available | 2020-09-04T13:28:09Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | International Journal of Molecular Sciences, 2020, v. 21 n. 17, p. article no. 6159 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/10722/286620 | - |
dc.description.abstract | Post-implantation cell survival and angio-/vasculogenesis are critical for the success of cell-based regenerative strategies. The current study aimed to overexpress B-cell lymphoma 2 (Bcl-2) gene in dental pulp stem cells (DPSCs) and examine the anti-apoptotic and angio-/vasculogenic effects both in-vitro and in-vivo. DPSCs were transduced with Bcl-2-green fluorescent protein (GFP) lentiviral particles and examined for cell proliferation and apoptosis. The cells were cultured under normoxic or hypoxic (0.5 mM CoCl2) conditions and examined for the expression of angiogenic factors and effects on endothelial cell proliferation, migration and vessel morphogenesis. Cells with or without hypoxic preconditioning were used in in-vivo Matrigel plug assay to study the post-implantation cell survival and angio-/vasculogenesis. Bcl-2-overexpressing-DPSCs showed significantly lower apoptosis than that of null-GFP-DPSCs under serum-free conditions. Under hypoxia, Bcl-2-overexpressing-DPSCs expressed significantly higher levels of vascular endothelial growth factor compared to that under normoxia and null-GFP-DPSCs. Consequently, Bcl-2-overexpressing-DPSCs significantly enhanced endothelial cell proliferation, migration and vascular tube formation on Matrigel. Immunohistological assessment of in-vivo transplanted Matrigel plugs showed significantly higher cell survival and vasculature in hypoxic preconditioned Bcl-2-overexpressing-DPSC group compared to null-GFP-DPSC group. In conclusion, Bcl-2 overexpression and hypoxic-preconditioning could be synergistically used to enhance post-implantation cell survival and angio-/vasculogenic properties of DPSCs. | - |
dc.language | eng | - |
dc.publisher | Molecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.org/ijms | - |
dc.relation.ispartof | International Journal of Molecular Sciences | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Bcl-2 | - |
dc.subject | gene modification | - |
dc.subject | angiogenesis | - |
dc.subject | post-implantation cell survival | - |
dc.subject | vascularization | - |
dc.title | Bcl-2 Overexpression and Hypoxia Synergistically Enhance Angiogenic Properties of Dental Pulp Stem Cells | - |
dc.type | Article | - |
dc.identifier.email | Dissanayaka, WL: warunad@hku.hk | - |
dc.identifier.email | Zou, T: zouting6@hku.hk | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.authority | Dissanayaka, WL=rp02216 | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3390/ijms21176159 | - |
dc.identifier.pmid | 32859045 | - |
dc.identifier.pmcid | PMC7503706 | - |
dc.identifier.scopus | eid_2-s2.0-85089848818 | - |
dc.identifier.hkuros | 314087 | - |
dc.identifier.volume | 21 | - |
dc.identifier.issue | 17 | - |
dc.identifier.spage | article no. 6159 | - |
dc.identifier.epage | article no. 6159 | - |
dc.identifier.isi | WOS:000571176400001 | - |
dc.publisher.place | Switzerland | - |
dc.identifier.issnl | 1422-0067 | - |