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Article: A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets

TitleA Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets
Authors
Zhou, PChan, BKCWan, YKYUEN, CTLChoi, GCGLI, XTong, CSWZhong, SSWSun, JBao, YMak, SYLChow, MZYKhaw, JVLeung, SYZheng, ZCheung, LWTTan, KWong, KHChan, HYEWong, ASL
Keywordscombinatorial genetics
CombiGEM
CRISPR
combination therapy
high-throughput screening
Issue Date2020
PublisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports
Citation
Cell Reports, 2020, v. 32 n. 6, p. article no. 108020 How to Cite?
DOI: http://dx.doi.org/10.1016/j.celrep.2020.108020
AbstractWe present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying not only pairwise but also three-way synergistic therapeutic target combinations and successfully validate double- and triple-combination regimens for suppression of cancer cell growth and protection against Parkinson's disease-associated toxicity. This system overcomes the practical challenges of experimenting on a large number of high-order genetic and drug combinations and can be applied to uncover the rare synergistic interactions between druggable targets.
Persistent Identifierhttp://hdl.handle.net/10722/287249
ISSN
2211-1247
2021 Impact Factor: 9.995
2020 SCImago Journal Rankings: 6.264
ISI Accession Number ID
WOS:000560042600017

 

DC FieldValueLanguage
dc.contributor.authorZhou, P-
dc.contributor.authorChan, BKC-
dc.contributor.authorWan, YK-
dc.contributor.authorYUEN, CTL-
dc.contributor.authorChoi, GCG-
dc.contributor.authorLI, X-
dc.contributor.authorTong, CSW-
dc.contributor.authorZhong, SSW-
dc.contributor.authorSun, J-
dc.contributor.authorBao, Y-
dc.contributor.authorMak, SYL-
dc.contributor.authorChow, MZY-
dc.contributor.authorKhaw, JV-
dc.contributor.authorLeung, SY-
dc.contributor.authorZheng, Z-
dc.contributor.authorCheung, LWT-
dc.contributor.authorTan, K-
dc.contributor.authorWong, KH-
dc.contributor.authorChan, HYE-
dc.contributor.authorWong, ASL-
dc.date.accessioned2020-09-22T02:58:07Z-
dc.date.available2020-09-22T02:58:07Z-
dc.date.issued2020-
dc.identifier.citationCell Reports, 2020, v. 32 n. 6, p. article no. 108020-
dc.identifier.issn2211-1247-
dc.identifier.urihttp://hdl.handle.net/10722/287249-
dc.description.abstractWe present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying not only pairwise but also three-way synergistic therapeutic target combinations and successfully validate double- and triple-combination regimens for suppression of cancer cell growth and protection against Parkinson's disease-associated toxicity. This system overcomes the practical challenges of experimenting on a large number of high-order genetic and drug combinations and can be applied to uncover the rare synergistic interactions between druggable targets.-
dc.languageeng-
dc.publisherElsevier (Cell Press): OAJ. The Journal's web site is located at http://cell.com/cell-reports-
dc.relation.ispartofCell Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectcombinatorial genetics-
dc.subjectCombiGEM-
dc.subjectCRISPR-
dc.subjectcombination therapy-
dc.subjecthigh-throughput screening-
dc.titleA Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets-
dc.typeArticle-
dc.identifier.emailZhou, P: zhoupeng@hku.hk-
dc.identifier.emailChan, BKC: beckyckc@hku.hk-
dc.identifier.emailChoi, GCG: gigichoi@hku.hk-
dc.identifier.emailTong, CSW: cswtong@hku.hk-
dc.identifier.emailZhong, SSW: u3536772@connect.hku.hk-
dc.identifier.emailSun, J: sunjr@HKUCC-COM.hku.hk-
dc.identifier.emailLeung, SY: suetyi@hku.hk-
dc.identifier.emailCheung, LWT: lydiacwt@hku.hk-
dc.identifier.emailWong, ASL: aslw@hku.hk-
dc.identifier.authorityLeung, SY=rp00359-
dc.identifier.authorityCheung, LWT=rp02137-
dc.identifier.authorityWong, ASL=rp02139-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.celrep.2020.108020-
dc.identifier.pmid32783942-
dc.identifier.scopuseid_2-s2.0-85089183838-
dc.identifier.hkuros314202-
dc.identifier.volume32-
dc.identifier.issue6-
dc.identifier.spagearticle no. 108020-
dc.identifier.epagearticle no. 108020-
dc.identifier.isiWOS:000560042600017-
dc.publisher.placeUnited States-
dc.identifier.issnl2211-1247-

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