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Conference Paper: Growth Factors and Small-molecule Compounds in Derivation of Endothelial Lineages from Dental Stem Cells

TitleGrowth Factors and Small-molecule Compounds in Derivation of Endothelial Lineages from Dental Stem Cells
Authors
KeywordsDental stem cells
endothelial cells
growth factors
reprogramming
small molecules
Issue Date2020
PublisherElsevier Inc. The Journal's web site is located at http://www.jendodon.com
Citation
Pulp Biology and Regeneration Group Satellite Meeting: Basic and Translational Research in Pulp Biology – Developing Technologies for Regenerating Vital Dental Tissues, Portland, OR, 23-25 June 2019. In Journal of Endodontics, v. 46 n. 9, suppl., p. S63-S70 How to Cite?
AbstractIntroduction: Incorporating fully assembled microvascular networks into bioengineered dental pulp constructs can significantly enhance functional blood flow and tissue survival upon transplantation. Endothelial cells (ECs), cellular building blocks of vascular tissue, play an essential role in the process of prevascularization. However, obtaining sufficient ECs from a suitable source for translational application is challenging. Dental stem cells (DSCs), which exhibit a robust proliferative ability and immunocompatibility because of their autologous origin, could be a promising alternative cell source for the derivation of endothelial lineages. Under specific culture conditions, DSCs differentiate into osteo/odontogenic, adipogenic, chondrogenic, and neurogenic cell lineages. Methods: Recently, a new approach has been developed to directly reprogram cells using chemical cocktails and growth factors. Compared with the traditional reprogramming approach based on the forced expression of exogenous transcription factors, the chemical strategy avoids the risk associated with lentiviral transduction while offering a more viable methodology to drive cell lineage switch. The aim of this review was to unveil the concept of the use of small-molecule compounds and growth factors modulating key signaling pathways to derive ECs from DSCs. Results: In addition, our preliminary study showed that stem cells from the apical papilla could be induced into EC-like cells using small-molecule compounds and growth factors. These EC-like cells expressed endothelial specific genes (CD31 and VEGFR2) and proteins (CD31, VEGF receptor 2, and vascular endothelial cadherin) as well as gave rise to vessel-like tubular structures in vitro. Conclusions: Our preliminary results suggest that chemical reprogramming might offer a novel way to generate EC-like cells from dental stem cells.
Persistent Identifierhttp://hdl.handle.net/10722/288016
ISSN
2023 Impact Factor: 3.5
2023 SCImago Journal Rankings: 1.356
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYI, B-
dc.contributor.authorDissanayaka, WL-
dc.contributor.authorZhang, C-
dc.date.accessioned2020-10-05T12:06:37Z-
dc.date.available2020-10-05T12:06:37Z-
dc.date.issued2020-
dc.identifier.citationPulp Biology and Regeneration Group Satellite Meeting: Basic and Translational Research in Pulp Biology – Developing Technologies for Regenerating Vital Dental Tissues, Portland, OR, 23-25 June 2019. In Journal of Endodontics, v. 46 n. 9, suppl., p. S63-S70-
dc.identifier.issn0099-2399-
dc.identifier.urihttp://hdl.handle.net/10722/288016-
dc.description.abstractIntroduction: Incorporating fully assembled microvascular networks into bioengineered dental pulp constructs can significantly enhance functional blood flow and tissue survival upon transplantation. Endothelial cells (ECs), cellular building blocks of vascular tissue, play an essential role in the process of prevascularization. However, obtaining sufficient ECs from a suitable source for translational application is challenging. Dental stem cells (DSCs), which exhibit a robust proliferative ability and immunocompatibility because of their autologous origin, could be a promising alternative cell source for the derivation of endothelial lineages. Under specific culture conditions, DSCs differentiate into osteo/odontogenic, adipogenic, chondrogenic, and neurogenic cell lineages. Methods: Recently, a new approach has been developed to directly reprogram cells using chemical cocktails and growth factors. Compared with the traditional reprogramming approach based on the forced expression of exogenous transcription factors, the chemical strategy avoids the risk associated with lentiviral transduction while offering a more viable methodology to drive cell lineage switch. The aim of this review was to unveil the concept of the use of small-molecule compounds and growth factors modulating key signaling pathways to derive ECs from DSCs. Results: In addition, our preliminary study showed that stem cells from the apical papilla could be induced into EC-like cells using small-molecule compounds and growth factors. These EC-like cells expressed endothelial specific genes (CD31 and VEGFR2) and proteins (CD31, VEGF receptor 2, and vascular endothelial cadherin) as well as gave rise to vessel-like tubular structures in vitro. Conclusions: Our preliminary results suggest that chemical reprogramming might offer a novel way to generate EC-like cells from dental stem cells.-
dc.languageeng-
dc.publisherElsevier Inc. The Journal's web site is located at http://www.jendodon.com-
dc.relation.ispartofJournal of Endodontics-
dc.subjectDental stem cells-
dc.subjectendothelial cells-
dc.subjectgrowth factors-
dc.subjectreprogramming-
dc.subjectsmall molecules-
dc.titleGrowth Factors and Small-molecule Compounds in Derivation of Endothelial Lineages from Dental Stem Cells-
dc.typeConference_Paper-
dc.identifier.emailDissanayaka, WL: warunad@hku.hk-
dc.identifier.emailZhang, C: zhangcf@hku.hk-
dc.identifier.authorityDissanayaka, WL=rp02216-
dc.identifier.authorityZhang, C=rp01408-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.joen.2020.06.024-
dc.identifier.pmid32950197-
dc.identifier.scopuseid_2-s2.0-85090993246-
dc.identifier.hkuros314942-
dc.identifier.volume46-
dc.identifier.issue9, suppl.-
dc.identifier.spageS63-
dc.identifier.epageS70-
dc.identifier.isiWOS:000573413200010-
dc.publisher.placeUnited States-
dc.identifier.issnl0099-2399-

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