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- Publisher Website: 10.1007/978-1-4939-8885-3_5
- Scopus: eid_2-s2.0-85056947717
- PMID: 30465196
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Article: Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors
Title | Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors |
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Authors | |
Keywords | scRNA-seq Innate lymphoid cells Cellular heterogeneity Immune cell development PD-1 |
Issue Date | 2019 |
Publisher | Humana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651 |
Citation | Methods in Molecular Biology, 2019, v. 1884, p. 73-86 How to Cite? |
Abstract | Single-cell RNA sequencing (scRNA-seq) is a powerful tool to study immune cells, which enables an unbiased way to discover novel cell populations, biological meaningful cellular heterogeneity, and cell lineage development trajectories. Advances in scRNA-seq technologies and computational data analysis have driven a revolution in our understanding of the immune system in health and disease. Technically, the key step for scRNA-seq analysis is making a high-quality cDNA library for sequencing. Here, we describe a plate-based protocol to prepare single-cell cDNA library of bone marrow innate lymphoid precursors for next generation sequencing-based transcriptome analysis. |
Description | Cover title - Cancer Immunosurveillance: methods and protocols |
Persistent Identifier | http://hdl.handle.net/10722/288407 |
ISBN | |
ISSN | 2023 SCImago Journal Rankings: 0.399 |
DC Field | Value | Language |
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dc.contributor.author | Yu, Y | - |
dc.contributor.author | Liu, P | - |
dc.date.accessioned | 2020-10-05T12:12:26Z | - |
dc.date.available | 2020-10-05T12:12:26Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Methods in Molecular Biology, 2019, v. 1884, p. 73-86 | - |
dc.identifier.isbn | 9781493988853 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | http://hdl.handle.net/10722/288407 | - |
dc.description | Cover title - Cancer Immunosurveillance: methods and protocols | - |
dc.description.abstract | Single-cell RNA sequencing (scRNA-seq) is a powerful tool to study immune cells, which enables an unbiased way to discover novel cell populations, biological meaningful cellular heterogeneity, and cell lineage development trajectories. Advances in scRNA-seq technologies and computational data analysis have driven a revolution in our understanding of the immune system in health and disease. Technically, the key step for scRNA-seq analysis is making a high-quality cDNA library for sequencing. Here, we describe a plate-based protocol to prepare single-cell cDNA library of bone marrow innate lymphoid precursors for next generation sequencing-based transcriptome analysis. | - |
dc.language | eng | - |
dc.publisher | Humana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651 | - |
dc.relation.ispartof | Methods in Molecular Biology | - |
dc.rights | This is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: https://doi.org/[insert DOI] | - |
dc.subject | scRNA-seq | - |
dc.subject | Innate lymphoid cells | - |
dc.subject | Cellular heterogeneity | - |
dc.subject | Immune cell development | - |
dc.subject | PD-1 | - |
dc.title | Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors | - |
dc.type | Article | - |
dc.identifier.email | Liu, P: pliu88@hku.hk | - |
dc.identifier.authority | Liu, P=rp02328 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/978-1-4939-8885-3_5 | - |
dc.identifier.pmid | 30465196 | - |
dc.identifier.scopus | eid_2-s2.0-85056947717 | - |
dc.identifier.hkuros | 315694 | - |
dc.identifier.volume | 1884 | - |
dc.identifier.spage | 73 | - |
dc.identifier.epage | 86 | - |
dc.identifier.eissn | 1940-6029 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1064-3745 | - |