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Article: Establishing Simultaneous T Cell Receptor Excision Circles (TREC) and K-Deleting Recombination Excision Circles (KREC) Quantification Assays and Laboratory Reference Intervals in Healthy Individuals of Different Age Groups in Hong Kong

TitleEstablishing Simultaneous T Cell Receptor Excision Circles (TREC) and K-Deleting Recombination Excision Circles (KREC) Quantification Assays and Laboratory Reference Intervals in Healthy Individuals of Different Age Groups in Hong Kong
Authors
KeywordsT cell receptor excision circles
K-deleting recombination excision circles
primary immunodeficiency
immune reconstitution
reference interval
Issue Date2020
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/immunology
Citation
Frontiers in Immunology, 2020, v. 11, p. article no. 1411 How to Cite?
AbstractThe clinical experience gathered throughout the years has raised awareness of primary immunodeficiency diseases (PIDD). T cell receptor excision circles (TREC) and kappa-deleting recombination excision circles (KREC) assays for thymic and bone marrow outputs measurement have been widely implemented in newborn screening (NBS) programs for Severe Combined Immunodeficiency. The potential applications of combined TREC and KREC assay in PIDD diagnosis and immune reconstitution monitoring in non-neonatal patients have been suggested. Given that ethnicity, gender, and age can contribute to variations in immunity, defining the reference intervals of TREC and KREC levels in the local population is crucial for setting up cut-offs for PIDD diagnosis. In this retrospective study, 479 healthy Chinese sibling donors (240 males and 239 females; age range: 1 month−74 years) from Hong Kong were tested for TREC and KREC levels using a simultaneous quantitative real-time PCR assay. Age-specific 5th–95th percentile reference intervals of TREC and KREC levels (expressed in copies per μL blood and copies per 106 cells) were established in both pediatric and adult age groups. Significant inverse correlations between age and both TREC and KREC levels were observed in the pediatric age group. A significant higher KREC level was observed in females than males after 9–12 years of age but not for TREC. Low TREC or KREC levels were detected in patients diagnosed with mild or severe PIDD. This assay with the established local reference intervals would allow accurate diagnosis of PIDD, and potentially monitoring immune reconstitution following haematopoietic stem cell transplantation or highly active anti-retroviral therapy in the future.
Persistent Identifierhttp://hdl.handle.net/10722/289466
ISSN
2019 Impact Factor: 5.085
2015 SCImago Journal Rankings: 2.810
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKwok, JSY-
dc.contributor.authorCheung, SKF-
dc.contributor.authorHo, JCY-
dc.contributor.authorTang, IWH-
dc.contributor.authorChu, PWK-
dc.contributor.authorLeung, EYS-
dc.contributor.authorLee, PPW-
dc.contributor.authorCheuk, KLD-
dc.contributor.authorLee, V-
dc.contributor.authorLau, YL-
dc.contributor.authorIp, P-
dc.date.accessioned2020-10-22T08:13:04Z-
dc.date.available2020-10-22T08:13:04Z-
dc.date.issued2020-
dc.identifier.citationFrontiers in Immunology, 2020, v. 11, p. article no. 1411-
dc.identifier.issn1664-3224-
dc.identifier.urihttp://hdl.handle.net/10722/289466-
dc.description.abstractThe clinical experience gathered throughout the years has raised awareness of primary immunodeficiency diseases (PIDD). T cell receptor excision circles (TREC) and kappa-deleting recombination excision circles (KREC) assays for thymic and bone marrow outputs measurement have been widely implemented in newborn screening (NBS) programs for Severe Combined Immunodeficiency. The potential applications of combined TREC and KREC assay in PIDD diagnosis and immune reconstitution monitoring in non-neonatal patients have been suggested. Given that ethnicity, gender, and age can contribute to variations in immunity, defining the reference intervals of TREC and KREC levels in the local population is crucial for setting up cut-offs for PIDD diagnosis. In this retrospective study, 479 healthy Chinese sibling donors (240 males and 239 females; age range: 1 month−74 years) from Hong Kong were tested for TREC and KREC levels using a simultaneous quantitative real-time PCR assay. Age-specific 5th–95th percentile reference intervals of TREC and KREC levels (expressed in copies per μL blood and copies per 106 cells) were established in both pediatric and adult age groups. Significant inverse correlations between age and both TREC and KREC levels were observed in the pediatric age group. A significant higher KREC level was observed in females than males after 9–12 years of age but not for TREC. Low TREC or KREC levels were detected in patients diagnosed with mild or severe PIDD. This assay with the established local reference intervals would allow accurate diagnosis of PIDD, and potentially monitoring immune reconstitution following haematopoietic stem cell transplantation or highly active anti-retroviral therapy in the future.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/immunology-
dc.relation.ispartofFrontiers in Immunology-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectT cell receptor excision circles-
dc.subjectK-deleting recombination excision circles-
dc.subjectprimary immunodeficiency-
dc.subjectimmune reconstitution-
dc.subjectreference interval-
dc.titleEstablishing Simultaneous T Cell Receptor Excision Circles (TREC) and K-Deleting Recombination Excision Circles (KREC) Quantification Assays and Laboratory Reference Intervals in Healthy Individuals of Different Age Groups in Hong Kong-
dc.typeArticle-
dc.identifier.emailKwok, JSY: ksy464@hku.hk-
dc.identifier.emailLee, PPW: ppwlee@hku.hk-
dc.identifier.emailCheuk, KLD: klcheuk@hkucc.hku.hk-
dc.identifier.emailLau, YL: lauylung@hku.hk-
dc.identifier.emailIp, P: patricip@hku.hk-
dc.identifier.authorityLee, PPW=rp00462-
dc.identifier.authorityLau, YL=rp00361-
dc.identifier.authorityIp, P=rp01337-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fimmu.2020.01411-
dc.identifier.pmid32765500-
dc.identifier.pmcidPMC7378446-
dc.identifier.scopuseid_2-s2.0-85088781508-
dc.identifier.hkuros316034-
dc.identifier.volume11-
dc.identifier.spagearticle no. 1411-
dc.identifier.epagearticle no. 1411-
dc.identifier.isiWOS:000556769200001-
dc.publisher.placeSwitzerland-
dc.identifier.issnl1664-3224-

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