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Article: Molecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia

TitleMolecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia
Authors
KeywordsMiddle East Respiratory Syndrome Coronavirus
Coronavirus Infections
Hajj
Issue Date2020
PublisherOxford University Press. The Journal's web site is located at http://www.clinchem.org
Citation
Clinical Chemistry, 2020, v. 66 n. 4, p. 549-555 How to Cite?
AbstractBackground: A novel coronavirus of zoonotic origin (2019-nCoV) has recently been identified in patients with acute respiratory disease. This virus is genetically similar to SARS coronavirus and bat SARS-like coronaviruses. The outbreak was initially detected in Wuhan, a major city of China, but has subsequently been detected in other provinces of China. Travel-associated cases have also been reported in a few other countries. Outbreaks in health care workers indicate human-to-human transmission. Molecular tests for rapid detection of this virus are urgently needed for early identification of infected patients. Methods: We developed two 1-step quantitative real-time reverse-transcription PCR assays to detect two different regions (ORF1b and N) of the viral genome. The primer and probe sets were designed to react with this novel coronavirus and its closely related viruses, such as SARS coronavirus. These assays were evaluated using a panel of positive and negative controls. In addition, respiratory specimens from two 2019-nCoV-infected patients were tested. Results: Using RNA extracted from cells infected by SARS coronavirus as a positive control, these assays were shown to have a dynamic range of at least seven orders of magnitude (2x10-4-2000 TCID50/reaction). Using DNA plasmids as positive standards, the detection limits of these assays were found to be below 10 copies per reaction. All negative control samples were negative in the assays. Samples from two 2019-nCoV-infected patients were positive in the tests. Conclusions: The established assays can achieve a rapid detection of 2019n-CoV in human samples, thereby allowing early identification of patients.
Persistent Identifierhttp://hdl.handle.net/10722/289548
ISSN
2023 Impact Factor: 7.1
2023 SCImago Journal Rankings: 1.460
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChu, DKW-
dc.contributor.authorPan, Y-
dc.contributor.authorCheng, SMS-
dc.contributor.authorHui, KPY-
dc.contributor.authorKrishnan, P-
dc.contributor.authorLiu, Y-
dc.contributor.authorNg, DYM-
dc.contributor.authorWan, CKC-
dc.contributor.authorYang, P-
dc.contributor.authorWang, Q-
dc.contributor.authorPeiris, M-
dc.contributor.authorPoon, LLM-
dc.date.accessioned2020-10-22T08:14:11Z-
dc.date.available2020-10-22T08:14:11Z-
dc.date.issued2020-
dc.identifier.citationClinical Chemistry, 2020, v. 66 n. 4, p. 549-555-
dc.identifier.issn0009-9147-
dc.identifier.urihttp://hdl.handle.net/10722/289548-
dc.description.abstractBackground: A novel coronavirus of zoonotic origin (2019-nCoV) has recently been identified in patients with acute respiratory disease. This virus is genetically similar to SARS coronavirus and bat SARS-like coronaviruses. The outbreak was initially detected in Wuhan, a major city of China, but has subsequently been detected in other provinces of China. Travel-associated cases have also been reported in a few other countries. Outbreaks in health care workers indicate human-to-human transmission. Molecular tests for rapid detection of this virus are urgently needed for early identification of infected patients. Methods: We developed two 1-step quantitative real-time reverse-transcription PCR assays to detect two different regions (ORF1b and N) of the viral genome. The primer and probe sets were designed to react with this novel coronavirus and its closely related viruses, such as SARS coronavirus. These assays were evaluated using a panel of positive and negative controls. In addition, respiratory specimens from two 2019-nCoV-infected patients were tested. Results: Using RNA extracted from cells infected by SARS coronavirus as a positive control, these assays were shown to have a dynamic range of at least seven orders of magnitude (2x10-4-2000 TCID50/reaction). Using DNA plasmids as positive standards, the detection limits of these assays were found to be below 10 copies per reaction. All negative control samples were negative in the assays. Samples from two 2019-nCoV-infected patients were positive in the tests. Conclusions: The established assays can achieve a rapid detection of 2019n-CoV in human samples, thereby allowing early identification of patients.-
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://www.clinchem.org-
dc.relation.ispartofClinical Chemistry-
dc.rightsPre-print: Journal Title] ©: [year] [owner as specified on the article] Published by Oxford University Press [on behalf of xxxxxx]. All rights reserved. Pre-print (Once an article is published, preprint notice should be amended to): This is an electronic version of an article published in [include the complete citation information for the final version of the Article as published in the print edition of the Journal.] Post-print: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in [insert journal title] following peer review. The definitive publisher-authenticated version [insert complete citation information here] is available online at: xxxxxxx [insert URL that the author will receive upon publication here]. -
dc.subjectMiddle East Respiratory Syndrome Coronavirus-
dc.subjectCoronavirus Infections-
dc.subjectHajj-
dc.titleMolecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia-
dc.typeArticle-
dc.identifier.emailChu, DKW: dkwchu@hku.hk-
dc.identifier.emailCheng, SMS: samuelms@hku.hk-
dc.identifier.emailHui, KPY: kenrie@hku.hk-
dc.identifier.emailKrishnan, P: pavithra@hku.hk-
dc.identifier.emailLiu, Y: gigilyz@hku.hk-
dc.identifier.emailNg, DYM: daisy34@hku.hk-
dc.identifier.emailWan, CKC: carriekc@hku.hk-
dc.identifier.emailPeiris, M: malik@hkucc.hku.hk-
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hk-
dc.identifier.authorityChu, DKW=rp02512-
dc.identifier.authorityHui, KPY=rp02149-
dc.identifier.authorityPeiris, M=rp00410-
dc.identifier.authorityPoon, LLM=rp00484-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/clinchem/hvaa029-
dc.identifier.pmid32031583-
dc.identifier.pmcidPMC7108203-
dc.identifier.scopuseid_2-s2.0-85080137262-
dc.identifier.hkuros317035-
dc.identifier.volume66-
dc.identifier.issue4-
dc.identifier.spage549-
dc.identifier.epage555-
dc.identifier.isiWOS:000522728500010-
dc.publisher.placeUnited States-
dc.identifier.issnl0009-9147-

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