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Book Chapter: FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures

TitleFRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures
Authors
KeywordsDNA origami
DNA–protein interaction
Aptamers
Molecular logic gate
Issue Date2021
PublisherHumana.
Citation
FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures. In Ryadnov, MG (Ed.), Polypeptide Materials: Methods and Protocols, p. 69-80. New York, NY: Humana, 2021 How to Cite?
AbstractDNA origami is a powerful technique, which allows virtually limitless 2D or 3D nanostructure designs to be constructed from DNA strands. Such nanostructures can even include programmable nanorobots, which are able to respond to the environment in predetermined ways. DNA aptamers hold particular promise as interfaces, which can enable proteins, peptides, and other non-nucleic acid biomolecules to trigger conformational changes in DNA nanostructures for diagnostic, biosensing, or therapeutic applications. Here, we provide the methodology for FRET-mediated observation of aptamer-triggered conformational change in a DNA origami box nanostructure. The method described can, in principle, be adapted to a wide variety of experimental circumstances where the DNA nanostructure conformational change is mediated by molecular or environmental cues.
Persistent Identifierhttp://hdl.handle.net/10722/289587
ISBN
ISSN
2023 SCImago Journal Rankings: 0.399
Series/Report no.Methods in Molecular Biology ; 2208

 

DC FieldValueLanguage
dc.contributor.authorShiu, SC-
dc.contributor.authorSakai, Y-
dc.contributor.authorTanner, JA-
dc.contributor.authorHeddle, JG-
dc.date.accessioned2020-10-22T08:14:43Z-
dc.date.available2020-10-22T08:14:43Z-
dc.date.issued2021-
dc.identifier.citationFRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures. In Ryadnov, MG (Ed.), Polypeptide Materials: Methods and Protocols, p. 69-80. New York, NY: Humana, 2021-
dc.identifier.isbn9781071609279-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/289587-
dc.description.abstractDNA origami is a powerful technique, which allows virtually limitless 2D or 3D nanostructure designs to be constructed from DNA strands. Such nanostructures can even include programmable nanorobots, which are able to respond to the environment in predetermined ways. DNA aptamers hold particular promise as interfaces, which can enable proteins, peptides, and other non-nucleic acid biomolecules to trigger conformational changes in DNA nanostructures for diagnostic, biosensing, or therapeutic applications. Here, we provide the methodology for FRET-mediated observation of aptamer-triggered conformational change in a DNA origami box nanostructure. The method described can, in principle, be adapted to a wide variety of experimental circumstances where the DNA nanostructure conformational change is mediated by molecular or environmental cues.-
dc.languageeng-
dc.publisherHumana.-
dc.relation.ispartofPolypeptide Materials: Methods and Protocols-
dc.relation.ispartofseriesMethods in Molecular Biology ; 2208-
dc.subjectDNA origami-
dc.subjectDNA–protein interaction-
dc.subjectAptamers-
dc.subjectMolecular logic gate-
dc.titleFRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures-
dc.typeBook_Chapter-
dc.identifier.emailTanner, JA: jatanner@hkucc.hku.hk-
dc.identifier.authorityTanner, JA=rp00495-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-0716-0928-6_5-
dc.identifier.pmid32856256-
dc.identifier.scopuseid_2-s2.0-85090001181-
dc.identifier.hkuros316965-
dc.identifier.hkuros328163-
dc.identifier.spage69-
dc.identifier.epage80-
dc.publisher.placeNew York, NY-
dc.identifier.issnl1064-3745-

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