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Article: Polyethylene glycol-mediated blocking and monolayer morphology of an electrochemical aptasensor for malaria biomarker detection in human serum

TitlePolyethylene glycol-mediated blocking and monolayer morphology of an electrochemical aptasensor for malaria biomarker detection in human serum
Authors
KeywordsMalaria detection
Electrochemical aptasensor
Human serum
Polyethylene glycol blocking
Blocking optimization
Issue Date2020
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bioelechem
Citation
Bioelectrochemistry, 2020, v. 136, p. article no. 107589 How to Cite?
AbstractBetter approaches are critically needed for in situ point-of-care diagnostic biosensors that enable primary care physicians, or even individual patients, to directly analyze biological fluids without complicated sample pretreatments. Additional purification steps consume time, consume reagents, often require other equipment, and can introduce false-negative results. Biosensors have been modified with blocking molecules to reduce biofouling; however, the effectiveness relies on their chemical composition and morphology. Here, we used a polyethylene glycol film to suppress unspecific binding from human serum on an electrochemical malaria aptasensor. A detailed study of the variation of the chemical and morphological composition of the aptamer/polyethylene glycol mixed monolayer as a function of incubation time was conducted. Higher resistance to matrix biofouling was found for polyethylene glycol than for hydrophobic alkanethiol films. The best sensor performance was observed for intermediate polyethylene glycol immobilization times. With prolonged incubation, phase separation of aptamer, and polyethylene glycol molecules locally increased the aptamer density and thereby diminished the analyte binding capability. Remarkably, polyethylene glycols do not affect the aptasensor sensitivity but enhance the complex matrix tolerance, the dynamic range, and the limit of detection. Careful tuning of the blocking molecule immobilization is crucial to achieving high aptasensor performance and biofouling resistance.
Persistent Identifierhttp://hdl.handle.net/10722/289591
ISSN
2023 Impact Factor: 4.8
2023 SCImago Journal Rankings: 0.705
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFigueroa-Miranda, G-
dc.contributor.authorWu, C-
dc.contributor.authorZhang, Y-
dc.contributor.authorNörbel, L-
dc.contributor.authorLO, Y-
dc.contributor.authorTanner, JA-
dc.contributor.authorElling, L-
dc.contributor.authorOffenhäusser, A-
dc.contributor.authorMayer, D-
dc.date.accessioned2020-10-22T08:14:47Z-
dc.date.available2020-10-22T08:14:47Z-
dc.date.issued2020-
dc.identifier.citationBioelectrochemistry, 2020, v. 136, p. article no. 107589-
dc.identifier.issn1567-5394-
dc.identifier.urihttp://hdl.handle.net/10722/289591-
dc.description.abstractBetter approaches are critically needed for in situ point-of-care diagnostic biosensors that enable primary care physicians, or even individual patients, to directly analyze biological fluids without complicated sample pretreatments. Additional purification steps consume time, consume reagents, often require other equipment, and can introduce false-negative results. Biosensors have been modified with blocking molecules to reduce biofouling; however, the effectiveness relies on their chemical composition and morphology. Here, we used a polyethylene glycol film to suppress unspecific binding from human serum on an electrochemical malaria aptasensor. A detailed study of the variation of the chemical and morphological composition of the aptamer/polyethylene glycol mixed monolayer as a function of incubation time was conducted. Higher resistance to matrix biofouling was found for polyethylene glycol than for hydrophobic alkanethiol films. The best sensor performance was observed for intermediate polyethylene glycol immobilization times. With prolonged incubation, phase separation of aptamer, and polyethylene glycol molecules locally increased the aptamer density and thereby diminished the analyte binding capability. Remarkably, polyethylene glycols do not affect the aptasensor sensitivity but enhance the complex matrix tolerance, the dynamic range, and the limit of detection. Careful tuning of the blocking molecule immobilization is crucial to achieving high aptasensor performance and biofouling resistance.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bioelechem-
dc.relation.ispartofBioelectrochemistry-
dc.subjectMalaria detection-
dc.subjectElectrochemical aptasensor-
dc.subjectHuman serum-
dc.subjectPolyethylene glycol blocking-
dc.subjectBlocking optimization-
dc.titlePolyethylene glycol-mediated blocking and monolayer morphology of an electrochemical aptasensor for malaria biomarker detection in human serum-
dc.typeArticle-
dc.identifier.emailTanner, JA: jatanner@hkucc.hku.hk-
dc.identifier.authorityTanner, JA=rp00495-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bioelechem.2020.107589-
dc.identifier.pmid32679336-
dc.identifier.scopuseid_2-s2.0-85087924638-
dc.identifier.hkuros316971-
dc.identifier.volume136-
dc.identifier.spagearticle no. 107589-
dc.identifier.epagearticle no. 107589-
dc.identifier.isiWOS:000579732600013-
dc.publisher.placeNetherlands-
dc.identifier.issnl1567-5394-

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