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Article: Lysozyme-Assisted Photothermal Eradication of Methicillin-Resistant Staphylococcus aureus Infection and Accelerated Tissue Repair with Natural Melanosome Nanostructures

TitleLysozyme-Assisted Photothermal Eradication of Methicillin-Resistant Staphylococcus aureus Infection and Accelerated Tissue Repair with Natural Melanosome Nanostructures
Authors
Keywordshuman hair
biodegradable
antibiotic-resistant bacterial infections
collagen alpha chain proteins
tissue repair
Issue Date2019
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/ancac3/index.html
Citation
ACS Nano, 2019, v. 13 n. 10, p. 11153-11167 How to Cite?
AbstractPatients often face the challenge of antibiotic-resistant bacterial infections and lengthy tissue reconstruction after surgery. Herein, human hair-melanosome derivatives (HHMs), comprising keratins and melanins, are developed using a simple “low-temperature alkali heat” method for potentially personalized therapy. The mulberry-shaped HHMs have an average width of ∼270 nm and an average length of ∼700 nm, and the negatively charged HHMs can absorb positively charged Lysozyme (Lyso) to form the HHMs-Lyso composites through electrostatic interaction. These naturally derived biodegradable nanostructures act as exogenous killers to eliminate methicillin-resistant Staphylococcus aureus (MRSA) infection with a high antibacterial efficacy (97.19 ± 2.39%) by synergistic action of photothermy and “Lyso-assisted anti-infection” in vivo. Additionally, HHMs also serve as endogenous regulators of collagen alpha chain proteins through the “protein digestion and absorption” signaling pathway to promote tissue reconstruction, which was confirmed by quantitative proteomic analysis in vivo. Notably, the 13 upregulated collagen alpha chain proteins in the extracellular matrix (ECM) after HHMs treatment demonstrated that keratin from HHMs in collagen-dependent regulatory processes serves as a notable contributor to augmented wound closure. The current paradigm of natural material–tissue interaction regulates the cell–ECM interaction by targeting cell signaling pathways to accelerate tissue repair. This work may provide insight into the protein-level pathways and the potential mechanisms involved in tissue repair.
Persistent Identifierhttp://hdl.handle.net/10722/289808
ISSN
2019 Impact Factor: 14.588
2015 SCImago Journal Rankings: 7.120
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLI, J-
dc.contributor.authorLIU, X-
dc.contributor.authorZHOU, Z-
dc.contributor.authorTAN, L-
dc.contributor.authorWANG, X-
dc.contributor.authorZHENG, Y-
dc.contributor.authorHAN, Y-
dc.contributor.authorCHEN, DF-
dc.contributor.authorYeung, KWK-
dc.contributor.authorCUI, Z-
dc.contributor.authorYANG, X-
dc.contributor.authorLIANG, Y-
dc.contributor.authorLI, Z-
dc.contributor.authorZHU, S-
dc.contributor.authorWU, S-
dc.date.accessioned2020-10-22T08:17:46Z-
dc.date.available2020-10-22T08:17:46Z-
dc.date.issued2019-
dc.identifier.citationACS Nano, 2019, v. 13 n. 10, p. 11153-11167-
dc.identifier.issn1936-0851-
dc.identifier.urihttp://hdl.handle.net/10722/289808-
dc.description.abstractPatients often face the challenge of antibiotic-resistant bacterial infections and lengthy tissue reconstruction after surgery. Herein, human hair-melanosome derivatives (HHMs), comprising keratins and melanins, are developed using a simple “low-temperature alkali heat” method for potentially personalized therapy. The mulberry-shaped HHMs have an average width of ∼270 nm and an average length of ∼700 nm, and the negatively charged HHMs can absorb positively charged Lysozyme (Lyso) to form the HHMs-Lyso composites through electrostatic interaction. These naturally derived biodegradable nanostructures act as exogenous killers to eliminate methicillin-resistant Staphylococcus aureus (MRSA) infection with a high antibacterial efficacy (97.19 ± 2.39%) by synergistic action of photothermy and “Lyso-assisted anti-infection” in vivo. Additionally, HHMs also serve as endogenous regulators of collagen alpha chain proteins through the “protein digestion and absorption” signaling pathway to promote tissue reconstruction, which was confirmed by quantitative proteomic analysis in vivo. Notably, the 13 upregulated collagen alpha chain proteins in the extracellular matrix (ECM) after HHMs treatment demonstrated that keratin from HHMs in collagen-dependent regulatory processes serves as a notable contributor to augmented wound closure. The current paradigm of natural material–tissue interaction regulates the cell–ECM interaction by targeting cell signaling pathways to accelerate tissue repair. This work may provide insight into the protein-level pathways and the potential mechanisms involved in tissue repair.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/ancac3/index.html-
dc.relation.ispartofACS Nano-
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].-
dc.subjecthuman hair-
dc.subjectbiodegradable-
dc.subjectantibiotic-resistant bacterial infections-
dc.subjectcollagen alpha chain proteins-
dc.subjecttissue repair-
dc.titleLysozyme-Assisted Photothermal Eradication of Methicillin-Resistant Staphylococcus aureus Infection and Accelerated Tissue Repair with Natural Melanosome Nanostructures-
dc.typeArticle-
dc.identifier.emailYeung, KWK: wkkyeung@hku.hk-
dc.identifier.authorityYeung, KWK=rp00309-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acsnano.9b03982-
dc.identifier.pmid31425647-
dc.identifier.scopuseid_2-s2.0-85072317832-
dc.identifier.hkuros317562-
dc.identifier.volume13-
dc.identifier.issue10-
dc.identifier.spage11153-
dc.identifier.epage11167-
dc.identifier.isiWOS:000492801600027-
dc.publisher.placeUnited States-
dc.identifier.issnl1936-0851-

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