File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1021/acsnano.9b03982
- Scopus: eid_2-s2.0-85072317832
- PMID: 31425647
- WOS: WOS:000492801600027
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Lysozyme-Assisted Photothermal Eradication of Methicillin-Resistant Staphylococcus aureus Infection and Accelerated Tissue Repair with Natural Melanosome Nanostructures
Title | Lysozyme-Assisted Photothermal Eradication of Methicillin-Resistant Staphylococcus aureus Infection and Accelerated Tissue Repair with Natural Melanosome Nanostructures |
---|---|
Authors | |
Keywords | human hair biodegradable antibiotic-resistant bacterial infections collagen alpha chain proteins tissue repair |
Issue Date | 2019 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/ancac3/index.html |
Citation | ACS Nano, 2019, v. 13 n. 10, p. 11153-11167 How to Cite? |
Abstract | Patients often face the challenge of antibiotic-resistant bacterial infections and lengthy tissue reconstruction after surgery. Herein, human hair-melanosome derivatives (HHMs), comprising keratins and melanins, are developed using a simple “low-temperature alkali heat” method for potentially personalized therapy. The mulberry-shaped HHMs have an average width of ∼270 nm and an average length of ∼700 nm, and the negatively charged HHMs can absorb positively charged Lysozyme (Lyso) to form the HHMs-Lyso composites through electrostatic interaction. These naturally derived biodegradable nanostructures act as exogenous killers to eliminate methicillin-resistant Staphylococcus aureus (MRSA) infection with a high antibacterial efficacy (97.19 ± 2.39%) by synergistic action of photothermy and “Lyso-assisted anti-infection” in vivo. Additionally, HHMs also serve as endogenous regulators of collagen alpha chain proteins through the “protein digestion and absorption” signaling pathway to promote tissue reconstruction, which was confirmed by quantitative proteomic analysis in vivo. Notably, the 13 upregulated collagen alpha chain proteins in the extracellular matrix (ECM) after HHMs treatment demonstrated that keratin from HHMs in collagen-dependent regulatory processes serves as a notable contributor to augmented wound closure. The current paradigm of natural material–tissue interaction regulates the cell–ECM interaction by targeting cell signaling pathways to accelerate tissue repair. This work may provide insight into the protein-level pathways and the potential mechanisms involved in tissue repair. |
Persistent Identifier | http://hdl.handle.net/10722/289808 |
ISSN | 2021 Impact Factor: 18.027 2020 SCImago Journal Rankings: 5.554 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | LI, J | - |
dc.contributor.author | LIU, X | - |
dc.contributor.author | ZHOU, Z | - |
dc.contributor.author | TAN, L | - |
dc.contributor.author | WANG, X | - |
dc.contributor.author | ZHENG, Y | - |
dc.contributor.author | HAN, Y | - |
dc.contributor.author | CHEN, DF | - |
dc.contributor.author | Yeung, KWK | - |
dc.contributor.author | CUI, Z | - |
dc.contributor.author | YANG, X | - |
dc.contributor.author | LIANG, Y | - |
dc.contributor.author | LI, Z | - |
dc.contributor.author | ZHU, S | - |
dc.contributor.author | WU, S | - |
dc.date.accessioned | 2020-10-22T08:17:46Z | - |
dc.date.available | 2020-10-22T08:17:46Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | ACS Nano, 2019, v. 13 n. 10, p. 11153-11167 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | http://hdl.handle.net/10722/289808 | - |
dc.description.abstract | Patients often face the challenge of antibiotic-resistant bacterial infections and lengthy tissue reconstruction after surgery. Herein, human hair-melanosome derivatives (HHMs), comprising keratins and melanins, are developed using a simple “low-temperature alkali heat” method for potentially personalized therapy. The mulberry-shaped HHMs have an average width of ∼270 nm and an average length of ∼700 nm, and the negatively charged HHMs can absorb positively charged Lysozyme (Lyso) to form the HHMs-Lyso composites through electrostatic interaction. These naturally derived biodegradable nanostructures act as exogenous killers to eliminate methicillin-resistant Staphylococcus aureus (MRSA) infection with a high antibacterial efficacy (97.19 ± 2.39%) by synergistic action of photothermy and “Lyso-assisted anti-infection” in vivo. Additionally, HHMs also serve as endogenous regulators of collagen alpha chain proteins through the “protein digestion and absorption” signaling pathway to promote tissue reconstruction, which was confirmed by quantitative proteomic analysis in vivo. Notably, the 13 upregulated collagen alpha chain proteins in the extracellular matrix (ECM) after HHMs treatment demonstrated that keratin from HHMs in collagen-dependent regulatory processes serves as a notable contributor to augmented wound closure. The current paradigm of natural material–tissue interaction regulates the cell–ECM interaction by targeting cell signaling pathways to accelerate tissue repair. This work may provide insight into the protein-level pathways and the potential mechanisms involved in tissue repair. | - |
dc.language | eng | - |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/ancac3/index.html | - |
dc.relation.ispartof | ACS Nano | - |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html]. | - |
dc.subject | human hair | - |
dc.subject | biodegradable | - |
dc.subject | antibiotic-resistant bacterial infections | - |
dc.subject | collagen alpha chain proteins | - |
dc.subject | tissue repair | - |
dc.title | Lysozyme-Assisted Photothermal Eradication of Methicillin-Resistant Staphylococcus aureus Infection and Accelerated Tissue Repair with Natural Melanosome Nanostructures | - |
dc.type | Article | - |
dc.identifier.email | Yeung, KWK: wkkyeung@hku.hk | - |
dc.identifier.authority | Yeung, KWK=rp00309 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1021/acsnano.9b03982 | - |
dc.identifier.pmid | 31425647 | - |
dc.identifier.scopus | eid_2-s2.0-85072317832 | - |
dc.identifier.hkuros | 317562 | - |
dc.identifier.volume | 13 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 11153 | - |
dc.identifier.epage | 11167 | - |
dc.identifier.isi | WOS:000492801600027 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1936-0851 | - |