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Article: Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

TitleDetection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Authors
KeywordsEuropean Union
human
information center
nonhuman
public health
Issue Date2020
PublisherEuropean Centre for Disease Prevention and Control. The Journal's web site is located at http://www.eurosurveillance.org/Public/AboutUs/AboutUs.aspx
Citation
Eurosurveillance, 2020, v. 25 n. 3, p. article no. 2000045 How to Cite?
AbstractBackground: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Methods: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. Results: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive– Global (EVAg), a European Union infrastructure project. Conclusion: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.
Persistent Identifierhttp://hdl.handle.net/10722/290018
ISSN
2021 Impact Factor: 21.286
2020 SCImago Journal Rankings: 2.766
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCorman, VN-
dc.contributor.authorLandt, O-
dc.contributor.authorKaiser, M-
dc.contributor.authorMolenkamp, R-
dc.contributor.authorMeijer, A-
dc.contributor.authorChu, DKW-
dc.contributor.authorBleicker, T-
dc.contributor.authorBrünink, S-
dc.contributor.authorSchneider, J-
dc.contributor.authorSchmidt, ML-
dc.contributor.authorMulders, DGJC-
dc.contributor.authorHaagmans, B-
dc.contributor.authorvan der Veer, B-
dc.contributor.authorvan den Brink, S-
dc.contributor.authorWijsman, L-
dc.contributor.authorGoderski, G-
dc.contributor.authorRomette, JL-
dc.contributor.authorEllis, J-
dc.contributor.authorZambon, M-
dc.contributor.authorPeiris, M-
dc.contributor.authorGoossens, H-
dc.contributor.authorReusken, C-
dc.contributor.authorKoopmans, MPG-
dc.contributor.authorDrosten, C-
dc.date.accessioned2020-10-22T08:20:49Z-
dc.date.available2020-10-22T08:20:49Z-
dc.date.issued2020-
dc.identifier.citationEurosurveillance, 2020, v. 25 n. 3, p. article no. 2000045-
dc.identifier.issn1025-496X-
dc.identifier.urihttp://hdl.handle.net/10722/290018-
dc.description.abstractBackground: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Methods: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. Results: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive– Global (EVAg), a European Union infrastructure project. Conclusion: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.-
dc.languageeng-
dc.publisherEuropean Centre for Disease Prevention and Control. The Journal's web site is located at http://www.eurosurveillance.org/Public/AboutUs/AboutUs.aspx-
dc.relation.ispartofEurosurveillance-
dc.rightsEurosurveillance. Copyright © European Centre for Disease Prevention and Control.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectEuropean Union-
dc.subjecthuman-
dc.subjectinformation center-
dc.subjectnonhuman-
dc.subjectpublic health-
dc.titleDetection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR-
dc.typeArticle-
dc.identifier.emailChu, DKW: dkwchu@hku.hk-
dc.identifier.emailPeiris, M: malik@hkucc.hku.hk-
dc.identifier.authorityChu, DKW=rp02512-
dc.identifier.authorityPeiris, M=rp00410-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.2807/1560-7917.ES.2020.25.3.2000045-
dc.identifier.pmid31992387-
dc.identifier.pmcidPMC6988269-
dc.identifier.scopuseid_2-s2.0-85078687750-
dc.identifier.hkuros317005-
dc.identifier.volume25-
dc.identifier.issue3-
dc.identifier.spagearticle no. 2000045-
dc.identifier.epagearticle no. 2000045-
dc.identifier.isiWOS:000509360400005-
dc.publisher.placeSweden-
dc.identifier.f1000737261495-
dc.identifier.issnl1025-496X-

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