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Article: Ultrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood

TitleUltrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood
Authors
Issue Date2020
PublisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html
Citation
Nature Communications, 2020, v. 11, p. article no. 6134 How to Cite?
AbstractDevelopment of plasmonic biosensors combining reliability and ease of use is still a challenge. Gold nanoparticle arrays made by block copolymer micelle nanolithography (BCMN) stand out for their scalability, cost-effectiveness and tunable plasmonic properties, making them ideal substrates for fluorescence enhancement. Here, we describe a plasmon-enhanced fluorescence immunosensor for the specific and ultrasensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH)—a malaria marker—in whole blood. Analyte recognition is realized by oriented antibodies immobilized in a close-packed configuration via the photochemical immobilization technique (PIT), with a top bioreceptor of nucleic acid aptamers recognizing a different surface of PfLDH in a sandwich conformation. The combination of BCMN and PIT enabled maximum control over the nanoparticle size and lattice constant as well as the distance of the fluorophore from the sensing surface. The device achieved a limit of detection smaller than 1 pg/mL (<30 fM) with very high specificity without any sample pretreatment. This limit of detection is several orders of magnitude lower than that found in malaria rapid diagnostic tests or even commercial ELISA kits. Thanks to its overall dimensions, ease of use and high-throughput analysis, the device can be used as a substrate in automated multi-well plate readers and improve the efficiency of conventional fluorescence immunoassays.
Persistent Identifierhttp://hdl.handle.net/10722/290509
ISSN
2023 Impact Factor: 14.7
2023 SCImago Journal Rankings: 4.887
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAntonio, M-
dc.contributor.authorBarolomeo, DV-
dc.contributor.authorLenyk, B-
dc.contributor.authorGentile, F-
dc.contributor.authorTanner, JA-
dc.contributor.authorOffenhausser, A-
dc.contributor.authorMayer, D-
dc.contributor.authorVelotta, R-
dc.date.accessioned2020-11-02T05:43:15Z-
dc.date.available2020-11-02T05:43:15Z-
dc.date.issued2020-
dc.identifier.citationNature Communications, 2020, v. 11, p. article no. 6134-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/10722/290509-
dc.description.abstractDevelopment of plasmonic biosensors combining reliability and ease of use is still a challenge. Gold nanoparticle arrays made by block copolymer micelle nanolithography (BCMN) stand out for their scalability, cost-effectiveness and tunable plasmonic properties, making them ideal substrates for fluorescence enhancement. Here, we describe a plasmon-enhanced fluorescence immunosensor for the specific and ultrasensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH)—a malaria marker—in whole blood. Analyte recognition is realized by oriented antibodies immobilized in a close-packed configuration via the photochemical immobilization technique (PIT), with a top bioreceptor of nucleic acid aptamers recognizing a different surface of PfLDH in a sandwich conformation. The combination of BCMN and PIT enabled maximum control over the nanoparticle size and lattice constant as well as the distance of the fluorophore from the sensing surface. The device achieved a limit of detection smaller than 1 pg/mL (<30 fM) with very high specificity without any sample pretreatment. This limit of detection is several orders of magnitude lower than that found in malaria rapid diagnostic tests or even commercial ELISA kits. Thanks to its overall dimensions, ease of use and high-throughput analysis, the device can be used as a substrate in automated multi-well plate readers and improve the efficiency of conventional fluorescence immunoassays.-
dc.languageeng-
dc.publisherNature Research: Fully open access journals. The Journal's web site is located at http://www.nature.com/ncomms/index.html-
dc.relation.ispartofNature Communications-
dc.rightsNature Communications. Copyright © Nature Research: Fully open access journals.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleUltrasensitive antibody-aptamer plasmonic biosensor for malaria biomarker detection in whole blood-
dc.typeArticle-
dc.identifier.emailTanner, JA: jatanner@hkucc.hku.hk-
dc.identifier.authorityTanner, JA=rp00495-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41467-020-19755-0-
dc.identifier.pmid33262332-
dc.identifier.pmcidPMC7708447-
dc.identifier.scopuseid_2-s2.0-85096960209-
dc.identifier.hkuros317648-
dc.identifier.volume11-
dc.identifier.spagearticle no. 6134-
dc.identifier.epagearticle no. 6134-
dc.identifier.isiWOS:000598899800001-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl2041-1723-

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