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- Publisher Website: 10.1016/j.ejphar.2019.172731
- Scopus: eid_2-s2.0-85073223949
- PMID: 31610186
- WOS: WOS:000500178300016
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Article: Flavonoids reduces lipopolysaccharide-induced release of inflammatory mediators in human bronchial epithelial cells: Structure-activity relationship
Title | Flavonoids reduces lipopolysaccharide-induced release of inflammatory mediators in human bronchial epithelial cells: Structure-activity relationship |
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Authors | |
Keywords | Bronchial epithelial cells Cyclic adenosine monophosphate Flavonoids Inflammation Lipopolysaccharide |
Issue Date | 2019 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar |
Citation | European Journal of Pharmacology, 2019, v. 865, p. article no. 172731 How to Cite? |
Abstract | Flavonoids are polyphenolic compounds that are widely present in food and Chinese medicine. The aim of the present study was to identify the flavonoids with anti-inflammatory effects in the airway; and to determine the role of anti-oxidant and cyclic adenosine monophosphate (cAMP) in the anti-inflammatory effect. Human bronchial epithelial BEAS-2B cells were exposed to bacterial endotoxin lipopolysaccharide (LPS) in the absence or presence of different flavonoids, which are categorized according to their chemical structures in seven subclasses [anthocyanidins, chalcones, flavanes, flavanones, flavones, flavonols, isoflavones]. Among the 17 flavonoids tested, only apigenin (flavones), luteolin (flavones), daidzein (isoflavones) and genistein (isoflavones) reduced LPS-induced release of inflammatory cytokines/chemokines interleukin (IL)-6, IL-8 and monocyte chemoattractant protein-1 in BEAS-2B cells. Quercetin caused further increase in LPS-induced IL-6 and IL-8 levels. It alone significantly increased nuclear factor-kappa B (NF-κB) p65 activity and the cellular oxidative stress marker malondialdehyde (MDA) level in BEAS-2B cells. By contrast, apigenin and genistein reduced LPS-induced increases in nuclear NF-κB activity and MDA level. Apigenin and genistein, but not quercetin, increased the cAMP level in BEAS-2B cells, and the cell-permeable cAMP analogue, 8-Br-cAMP, inhibited LPS-induced increase of IL-8 level. These findings suggest that the presence of C5-OH, C7-OH, C2=C3 and C4=O functional groups in the flavonoids is associated with greater anti-inflammatory effect, while that of C3-OH or glycosylation group at the A-ring greatly decreased the anti-inflammatory effect. The anti-inflammatory effect of these flavonoids may be related to their anti-oxidant properties, and partly to their ability in increasing cAMP level. |
Persistent Identifier | http://hdl.handle.net/10722/290933 |
ISSN | 2023 Impact Factor: 4.2 2023 SCImago Journal Rankings: 1.055 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | ZHANG, P | - |
dc.contributor.author | Mak, JCW | - |
dc.contributor.author | Man, RYK | - |
dc.contributor.author | Leung, SWS | - |
dc.date.accessioned | 2020-11-02T05:49:09Z | - |
dc.date.available | 2020-11-02T05:49:09Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | European Journal of Pharmacology, 2019, v. 865, p. article no. 172731 | - |
dc.identifier.issn | 0014-2999 | - |
dc.identifier.uri | http://hdl.handle.net/10722/290933 | - |
dc.description.abstract | Flavonoids are polyphenolic compounds that are widely present in food and Chinese medicine. The aim of the present study was to identify the flavonoids with anti-inflammatory effects in the airway; and to determine the role of anti-oxidant and cyclic adenosine monophosphate (cAMP) in the anti-inflammatory effect. Human bronchial epithelial BEAS-2B cells were exposed to bacterial endotoxin lipopolysaccharide (LPS) in the absence or presence of different flavonoids, which are categorized according to their chemical structures in seven subclasses [anthocyanidins, chalcones, flavanes, flavanones, flavones, flavonols, isoflavones]. Among the 17 flavonoids tested, only apigenin (flavones), luteolin (flavones), daidzein (isoflavones) and genistein (isoflavones) reduced LPS-induced release of inflammatory cytokines/chemokines interleukin (IL)-6, IL-8 and monocyte chemoattractant protein-1 in BEAS-2B cells. Quercetin caused further increase in LPS-induced IL-6 and IL-8 levels. It alone significantly increased nuclear factor-kappa B (NF-κB) p65 activity and the cellular oxidative stress marker malondialdehyde (MDA) level in BEAS-2B cells. By contrast, apigenin and genistein reduced LPS-induced increases in nuclear NF-κB activity and MDA level. Apigenin and genistein, but not quercetin, increased the cAMP level in BEAS-2B cells, and the cell-permeable cAMP analogue, 8-Br-cAMP, inhibited LPS-induced increase of IL-8 level. These findings suggest that the presence of C5-OH, C7-OH, C2=C3 and C4=O functional groups in the flavonoids is associated with greater anti-inflammatory effect, while that of C3-OH or glycosylation group at the A-ring greatly decreased the anti-inflammatory effect. The anti-inflammatory effect of these flavonoids may be related to their anti-oxidant properties, and partly to their ability in increasing cAMP level. | - |
dc.language | eng | - |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar | - |
dc.relation.ispartof | European Journal of Pharmacology | - |
dc.subject | Bronchial epithelial cells | - |
dc.subject | Cyclic adenosine monophosphate | - |
dc.subject | Flavonoids | - |
dc.subject | Inflammation | - |
dc.subject | Lipopolysaccharide | - |
dc.title | Flavonoids reduces lipopolysaccharide-induced release of inflammatory mediators in human bronchial epithelial cells: Structure-activity relationship | - |
dc.type | Article | - |
dc.identifier.email | Mak, JCW: judithmak@hku.hk | - |
dc.identifier.email | Leung, SWS: swsleung@hku.hk | - |
dc.identifier.authority | Mak, JCW=rp00352 | - |
dc.identifier.authority | Man, RYK=rp00236 | - |
dc.identifier.authority | Leung, SWS=rp00235 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.ejphar.2019.172731 | - |
dc.identifier.pmid | 31610186 | - |
dc.identifier.scopus | eid_2-s2.0-85073223949 | - |
dc.identifier.hkuros | 317945 | - |
dc.identifier.volume | 865 | - |
dc.identifier.spage | article no. 172731 | - |
dc.identifier.epage | article no. 172731 | - |
dc.identifier.isi | WOS:000500178300016 | - |
dc.publisher.place | Netherlands | - |
dc.identifier.issnl | 0014-2999 | - |