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Article: Costimulation of CD28- T Lymphocytes by 4-1BB Ligand

TitleCostimulation of CD28<sup>-</sup> T Lymphocytes by 4-1BB Ligand
Authors
Issue Date1997
Citation
Journal of Immunology, 1997, v. 158, n. 2, p. 551-559 How to Cite?
AbstractThe interaction of the T cell surface protein CD28 with its ligand, B7-1 or B7-2, provides a critical costimulatory signal for T cell activation. T cells from CD28- mice are deficient in a variety of responses, including those to lectins and allogeneic spleen cells. However, some immune responses do occur in CD28- mice, suggesting the existence of alternate costimulatory pathways. In this work, we show that T cells purified from CD28- mice respond to B lymphomas expressing 4-1BB ligand (4-1BBL), a member of the TNF gene family. This response is inhibited by a soluble form of 4-1BB, the T cell surface receptor for 4-1BBL. Thus, 4-1BBL/4-1BB interaction provides costimulatory signals to T cells independent of signaling through the CD28 receptor. We find that 4-1BBL is inducible on splenic B cells by CD40 ligand/CD40 interaction or by culturing of splenic dendritic cells, treatments that also induce B7 family molecules. CD28- T cells fail to respond in an MLR to resting allogeneic spleen cells. However, treatment of spleen cells with CD40 ligand renders them competent in activation of CD28- T cells. In contrast to results using B lymphomas as APC, soluble 4-1BB fails to inhibit the T cell response to activated spleen cells. This failure of soluble 4-1BB to block an MLR between CD28+ or CD28- T cells and allogeneic spleen cells is in contrast to a previous report with CD28+ cells.
Persistent Identifierhttp://hdl.handle.net/10722/291432
ISSN
2023 Impact Factor: 3.6
2023 SCImago Journal Rankings: 1.558
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDeBenedette, Mark A.-
dc.contributor.authorShahinian, Arda-
dc.contributor.authorMak, Tak W.-
dc.contributor.authorWatts, Tania H.-
dc.date.accessioned2020-11-17T14:54:21Z-
dc.date.available2020-11-17T14:54:21Z-
dc.date.issued1997-
dc.identifier.citationJournal of Immunology, 1997, v. 158, n. 2, p. 551-559-
dc.identifier.issn0022-1767-
dc.identifier.urihttp://hdl.handle.net/10722/291432-
dc.description.abstractThe interaction of the T cell surface protein CD28 with its ligand, B7-1 or B7-2, provides a critical costimulatory signal for T cell activation. T cells from CD28- mice are deficient in a variety of responses, including those to lectins and allogeneic spleen cells. However, some immune responses do occur in CD28- mice, suggesting the existence of alternate costimulatory pathways. In this work, we show that T cells purified from CD28- mice respond to B lymphomas expressing 4-1BB ligand (4-1BBL), a member of the TNF gene family. This response is inhibited by a soluble form of 4-1BB, the T cell surface receptor for 4-1BBL. Thus, 4-1BBL/4-1BB interaction provides costimulatory signals to T cells independent of signaling through the CD28 receptor. We find that 4-1BBL is inducible on splenic B cells by CD40 ligand/CD40 interaction or by culturing of splenic dendritic cells, treatments that also induce B7 family molecules. CD28- T cells fail to respond in an MLR to resting allogeneic spleen cells. However, treatment of spleen cells with CD40 ligand renders them competent in activation of CD28- T cells. In contrast to results using B lymphomas as APC, soluble 4-1BB fails to inhibit the T cell response to activated spleen cells. This failure of soluble 4-1BB to block an MLR between CD28+ or CD28- T cells and allogeneic spleen cells is in contrast to a previous report with CD28+ cells.-
dc.languageeng-
dc.relation.ispartofJournal of Immunology-
dc.titleCostimulation of CD28<sup>-</sup> T Lymphocytes by 4-1BB Ligand-
dc.typeArticle-
dc.identifier.pmid8992967-
dc.identifier.scopuseid_2-s2.0-0031567896-
dc.identifier.volume158-
dc.identifier.issue2-
dc.identifier.spage551-
dc.identifier.epage559-
dc.identifier.isiWOS:A1997WC63800003-
dc.identifier.issnl0022-1767-

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