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- Publisher Website: 10.1074/jbc.M210296200
- Scopus: eid_2-s2.0-0037518224
- PMID: 12519725
- WOS: WOS:000181777500093
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Article: BID-D59A is a potent inducer of apoptosis in primary embryonic fibroblasts
Title | BID-D59A is a potent inducer of apoptosis in primary embryonic fibroblasts |
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Authors | |
Issue Date | 2003 |
Citation | Journal of Biological Chemistry, 2003, v. 278, n. 12, p. 10707-10715 How to Cite? |
Abstract | The proapoptotic activity of BID seems to solely depend upon its cleavage to truncated tBID. Here we demonstrate that expression of a caspase-8 non-cleavable (nc) BID-D59A mutant or expression of wild type (wt) BID induces apoptosis in Bid -/-, caspase-8 -/-, and wt primary MEFs. Western blot analysis indicated that no cleavage products appeared in cells expressing ncBID. ncBID was as effective as wtBID in inducing cytochrome c release, caspase activation, and apoptosis, ncBID and wtBID (nc/wtBID) were much less effective than tBID in localizing to mitochondria and in inducing cytochrome c release, but only slightly less effective in inducing apoptosis. Studies with Apaf-1- and caspase-9-deficient primary MEFs indicated that both proteins were essential for nc/wtBID and for tBID-induced apoptosis. Most importantly, expression of non-apoptotic levels of either ncBID or wtBID in Bid -/- MEFs induced a similar and significant enhancement in apoptosis in response to a variety of death signals, which was accompanied by enhanced localization of BID to mitochondria and cytochrome c release. Thus, these results implicate full-length BID as an active player in the mitochondria during apoptosis. |
Persistent Identifier | http://hdl.handle.net/10722/291633 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID | |
Errata |
DC Field | Value | Language |
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dc.contributor.author | Sarig, Rachel | - |
dc.contributor.author | Zaltsman, Yehudit | - |
dc.contributor.author | Marcellus, Richard C. | - |
dc.contributor.author | Flavell, Richard | - |
dc.contributor.author | Mak, Tak W. | - |
dc.contributor.author | Gross, Atan | - |
dc.date.accessioned | 2020-11-17T14:54:47Z | - |
dc.date.available | 2020-11-17T14:54:47Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Journal of Biological Chemistry, 2003, v. 278, n. 12, p. 10707-10715 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10722/291633 | - |
dc.description.abstract | The proapoptotic activity of BID seems to solely depend upon its cleavage to truncated tBID. Here we demonstrate that expression of a caspase-8 non-cleavable (nc) BID-D59A mutant or expression of wild type (wt) BID induces apoptosis in Bid -/-, caspase-8 -/-, and wt primary MEFs. Western blot analysis indicated that no cleavage products appeared in cells expressing ncBID. ncBID was as effective as wtBID in inducing cytochrome c release, caspase activation, and apoptosis, ncBID and wtBID (nc/wtBID) were much less effective than tBID in localizing to mitochondria and in inducing cytochrome c release, but only slightly less effective in inducing apoptosis. Studies with Apaf-1- and caspase-9-deficient primary MEFs indicated that both proteins were essential for nc/wtBID and for tBID-induced apoptosis. Most importantly, expression of non-apoptotic levels of either ncBID or wtBID in Bid -/- MEFs induced a similar and significant enhancement in apoptosis in response to a variety of death signals, which was accompanied by enhanced localization of BID to mitochondria and cytochrome c release. Thus, these results implicate full-length BID as an active player in the mitochondria during apoptosis. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Biological Chemistry | - |
dc.title | BID-D59A is a potent inducer of apoptosis in primary embryonic fibroblasts | - |
dc.type | Article | - |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1074/jbc.M210296200 | - |
dc.identifier.pmid | 12519725 | - |
dc.identifier.scopus | eid_2-s2.0-0037518224 | - |
dc.identifier.volume | 278 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 10707 | - |
dc.identifier.epage | 10715 | - |
dc.identifier.isi | WOS:000181777500093 | - |
dc.relation.erratum | doi:10.1074/jbc.A112.210296 | - |
dc.relation.erratum | eid:eid_2-s2.0-84867421446 | - |
dc.relation.erratum | doi:10.1074/jbc.A113.210296 | - |
dc.relation.erratum | eid:eid_2-s2.0-84876583251 | - |
dc.identifier.issnl | 0021-9258 | - |